Identification of hexokinase family members in pear (Pyrus × bretschneideri) and functional exploration of PbHXK1 in modulating sugar content and plant growth

Genome-Wide Identification and Characterization of Hexokinase Genes in Moso Bamboo (Phyllostachys edulis)

“…To date, except for type D HXKs, which are mitochondrial proteins, have only been identified in the moss Physcomitrella patens (Nilsson et al, 2011), the other three types of HXKs are widely distributed in higher plants. Type A HXKs harboring a chloroplast signal at N-terminus commonly localized at chloroplasts and have been found in moss Physcomitrella patens and higher plants such as Arabidopsis thaliana, Nicotina tabacum, Oryza sativa, Solanum lycopersicum, Vitis vinifera, Camellia sinensis, Brassica napus, pear (Pyrus × bretschneideri), and Spinacia oleracea (Olsson et al, 2003;Cho et al, 2006;Kandel-Kfir et al, 2006;Karve et al, 2008Karve et al, , 2010Nilsson et al, 2011;Wang et al, 2018;Zhao et al, 2019). Type B HXKs, accounting for most members of HXK family in plants, have a highly hydrophobic helix and associates with mitochondria.…”

“…Using biochemical, genetic, or bioinformatics methods, plant HXK gene families have been identified in many species (Karve et al, 2010;Aguilera-Alvarado and Sanchez-Nieto, 2017), including Arabidopsis thaliana (Karve et al, 2008), Physcomitrella patens (Olsson et al, 2003), Lycopersicon esculentum (Damari-Weissler et al, 2006;Granot, 2007), Solanum lycopersicum (Kandel-Kfir et al, 2006), Nicotiana tabacum (Giese et al, 2005), Oryza sativa (Cho et al, 2006), Solanum tuberosum (Jon et al, 2002), Vitis vinifera (Fei et al, 2013), Zea mays (Zhang et al, 2014), Manihot esculenta Grantz (Geng et al, 2017) Camellia sinensis (Li et al, 2017), Brassica napus (Wang et al, 2018), and pear (Pyrus × bretschneideri) (Zhao et al, 2019). On the basis of N-terminal amino acid sequences, the hexokinases are classified into two main types (type A and type B), and two rare types (type C and type D) (Aguilera-Alvarado and Sanchez-Nieto, 2017).…”

“…In tomato, overexpression of AtHXK1 resulted in reduced photosynthesis, slower growth, and induction of senescence, which is indicative of its function in photosynthetic tissues (Dai et al, 1999). Overexpression of pear PbHXK1 in tomato modulated the sugar content and affected plant growth (Zhao et al, 2019). In Nicotiana benthamiana, virus-induced gene silencing of NbHXK1 caused cell death, implying that HXK1 plays a role in the process of cell death (Kim et al, 2006).…”

PdGNC confers drought tolerance by mediating stomatal closure resulting from NO and H2O2 production via the direct regulation of PdHXK1 expression in Populus

“…Hexokinase1 ( HXK1 ) is an evolutionarily conserved glucose sensor in plants, and has dual functions of glucose metabolism as well as glucose sensing and signaling (Granot & Kelly, 2019). HXK1 modulated stomatal movement and coped with abiotic stress by integrating metabolite and hormone signals (van Dingenen et al ., 2019; Zhao et al ., 2019; Zheng et al ., 2019). Previous studies have shown that sucrose triggered guard cell‐specific NO and H 2 O 2 production via hexokinase and ABA in tomato (Kelly et al ., 2013; Li et al ., 2018).…”

A genomic approach to the evolution, diversification and domestication of the genus Citrus

“…Re-sequencing data has also proved useful for detection of small insertions and deletions (indels), that have been used among other goals to identify Citrus chloroplast haplotypes and their phylogenetic relationships (Carbonell-Caballero et al, 2015;Maddi et al, 2018), as well as molecular markers for cultivar identification in mandarin hybrids (Noda et al, 2020). Currently, numerous analyses in Citrus have also been performed to detect mobile element insertions (Caruso et al, 2019;Liu et al, 2019). The relevance of these variants has been proved in other crops, where they have been linked to major agronomical traits (Zhang et al, 2015;Zhou et al, 2019;Alonge et al, 2020).…”