Identification of Heterodera glycines (Tylenchida; Heteroderidae) Using qPCR

Ko, Hyoung Rai; Kang, Heonil; Eun-Hyoung, Park，; Kim, Eun Hwa; Lee, Jae Kook

doi:10.5423/ppj.oa.04.2019.0086

Cited by 3 publications

(1 citation statement)

References 25 publications

(36 reference statements)

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“…Due to the absence of SCN in Taiwan, genotyping becomes the practical method to evaluate SCN resistance for the soybean varieties developed in Taiwan. While there were some publications claimed the presence of SCN in Taiwan [29][30][31][32], these literatures seemed to misunderstand an old literature [33] probably due to the language inconvenience. In the Hung's publication (Supplementary File 1), a table on the page 95 and the corresponding notes on the page 96 summarized several nematodes found in different fields of Pingtung County in Taiwan, which were based on the morphological characteristics.…”

Section: Discussionmentioning

confidence: 99%

Copy Number Quantification for the Soybean Cyst Nematode Resistance Locus rhg1 in the Soybean Varieties of Taiwan

et al. 2021

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Disease resistance is one of the most successful strategies in crop protection. For example, the implementation of PI 88788 type resistance, which contains high copy numbers of Resistance to Heterodera glycines 1 (rhg1) loci, into the commercial soybean varieties of the United States has significantly reduced the yield losses caused by soybean cyst nematode (SCN, H. glycines). Vegetable soybean, or edamame, has become a major exporting agricultural product in Taiwan with an annual revenue over $80 million USD since 2017. Several local varieties have been developed to fulfill the market needs such as the traits of flavor and sweetness. However, it remains unclear if the historical breeding programs ever incorporated rhg1 resistance into the varieties of Taiwan. This study applied the TaqMan qPCR method to measure the fluorescent signals specific to the rhg1 locus on the chromosome 18 of soybean, and the ratio of VIC and FAM signals were analyzed to predict the rhg1 copy number in the 21 soybean varieties of Taiwan. The results indicated the copy number and the single nucleotide polymorphisms of the 21 soybean varieties were identical to the susceptible soybean variety ‘Williams 82’. As importation of soybean will be continuously needed to fulfill the market and because SCN is absent in the soybean fields of Taiwan, lacking rhg1 resistance in the local soybean varieties may put the edamame industry at risk and early implementation of SCN resistance in the breeding program, alongside the application of quarantine regulations, will be the key to maintain the SCN-free status and to sustain the edamame industry in Taiwan.

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Section: Discussionmentioning

confidence: 99%

Copy Number Quantification for the Soybean Cyst Nematode Resistance Locus rhg1 in the Soybean Varieties of Taiwan

et al. 2021

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Soybean Cyst Nematode of Soybean: A Diagnostic Guide

Mahecha-Garnica

Schumacher

et al. 2022

Plant Health Progress

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The soybean cyst nematode (SCN; Heterodera glycines Ichinohe, 1952) is one of the most economically important pathogens of soybean. The species impacts soybean production worldwide and causes significant yield loss even in the absence of visible aboveground symptoms. This diagnostic guide describes the host range, signs, symptoms, and geographic distribution of SCN, as well as includes information regarding pathogen identification, storage, and pathogenicity tests for this nematode.

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Top Ten Most Important U.S.-Regulated and Emerging Plant-Parasitic Nematodes

et al. 2022

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Plant-parasitic nematodes (PPNs) are important pests that cause an estimated ten billion dollars of crop loss each year in the United States and over 100 billion dollars globally. The Animal and Plant Health Inspection Service (APHIS) within the U.S. Department of Agriculture maintains and updates the U.S. Regulated Plant Pest list. Currently, the number of PPNs regulated by APHIS includes more than 60 different species. This review focuses on the top ten most economically important regulated and emerging plant-parasitic nematodes and summarizes the diagnostics of morphological and some molecular features for distinguishing them. These ten major previously described nematode species are associated with various economically important crops from around the world. This review also includes their current distribution in the U.S. and a brief historical background and updated systematic position of these species. The species included in this review include three PPNs considered by the U.S. Department of Agriculture as invasive invertebrates Globodera pallida, Globodera rostochiensis, and Heterodera glycines; four regulated PPNs, namely Bursaphelenchus xylophilus, Meloidogyne fallax, Ditylenchus dipsaci, and Pratylenchus fallax; and the three emerging PPNs Meloidogyne chitwoodi, Meloidogyne enterolobii, and Litylenchus crenatae mccannii.

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Identification of Heterodera glycines (Tylenchida; Heteroderidae) Using qPCR

Cited by 3 publications

References 25 publications

Copy Number Quantification for the Soybean Cyst Nematode Resistance Locus rhg1 in the Soybean Varieties of Taiwan

Copy Number Quantification for the Soybean Cyst Nematode Resistance Locus rhg1 in the Soybean Varieties of Taiwan

Soybean Cyst Nematode of Soybean: A Diagnostic Guide

Top Ten Most Important U.S.-Regulated and Emerging Plant-Parasitic Nematodes

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