Identification of hemolytic activity in <i>Prevotella intermedia</i>

Beem, J E; Nesbitt, W E; Leung, K.-P.

doi:10.1111/j.1399-302x.1998.tb00719.x

Cited by 36 publications

(23 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The redox potential of hemin, required as a prosthetic group of cytochrome b, allows it to mediate electron transfer with generation of cellular energy (31,46). These bacteria, by virtue of their hemolytic activity, are capable of liberating the hemoglobin or hemin from erythrocytes, thereby acquiring the essential nutrient for their metabolism (44,47,48). It has been assumed that the high hemolytic and hemagglutinating activities of P. intermedia not only play a significant role in the growth of P. intermedia itself but also support the growth of other black-pigmented anaerobes, such as P. gingivalis, in subgingival sites (45).…”

Section: Discussionmentioning

confidence: 99%

In Vitro Effects of Polyphosphate against Prevotella intermedia in Planktonic Phase and Biofilm

Jang

Kim

Noh

et al. 2016

Antimicrob Agents Chemother

View full text Add to dashboard Cite

c Polyphosphate (polyP) has gained a wide interest in the food industry due to its potential as a decontaminating agent. In this study, we examined the effect of sodium tripolyphosphate (polyP3; Na 5 P 3 O 10 ) against planktonic and biofilm cells of Prevotella intermedia, a major oral pathogen. The MIC of polyP3 against P. intermedia ATCC 49046 determined by agar dilution method was 0.075%, while 0.05% polyP3 was bactericidal against P. intermedia in time-kill analysis performed using liquid medium. A crystal violet binding assay for the assessment of biofilm formation by P. intermedia showed that sub-MICs of polyP3 significantly decreased biofilm formation. Under the scanning electron microscope, decreased numbers of P. intermedia cells forming the biofilms were observed when the bacterial cells were incubated with 0.025% or higher concentrations of polyP3. Assessment of biofilm viability with LIVE/DEAD staining and viable cell count methods showed that 0.05% or higher concentrations of polyP3 significantly decreased the viability of the preformed biofilms in a concentration-dependent manner. The zone sizes of alpha-hemolysis formed on horse blood agar produced by P. intermedia were decreased in the presence of polyP3. The expression of the genes encoding hemolysins and the genes of the hemin uptake (hmu) locus was downregulated by polyP3. Collectively, our results show that polyP is an effective antimicrobial agent against P. intermedia in biofilms as well as planktonic phase, interfering with the process of hemin acquisition by the bacterium. P revotella intermedia is a black-pigmented anaerobic Gramnegative bacterium which has long been known to be associated with oral diseases, such as chronic periodontitis (1-3), aggressive periodontitis (4-6), puberty-associated gingivitis, acute necrotizing ulcerative gingivitis (7,8), periapical periodontitis (9, 10), and noma (an acute gangrenous disease) (11, 12). Besides being involved in oral diseases, P. intermedia has also been reported to be associated with various systemic diseases, such as cystic fibrosis, chronic bronchitis (13-15), and atherosclerosis (16). Difficulty in controlling the bacterium has been attributed to resistance of P. intermedia to many antibiotics, including penicillins, cephalosporins, and tetracyclines (17, 18). Moreover, P. intermedia cells form a biofilm in which the bacterial cells become more resistant to antibiotics (19). Because biofilm can serve as a reservoir of antibiotic resistance (20), it is of clinical significance to develop alternative antimicrobial approaches for controlling antibiotic-resistant P. intermedia.Inorganic polyphosphate (polyP) is a chain of few or many hundreds of phosphate (P i ) residues linked by high-energy phosphoanhydride (21). Intracellular polyP found in bacteria is considered a virulence factor since it performs various functions, such as serving as an ATP source and substitute, a regulator of the intracellular levels of metal ions, a channel for DNA entry, and a regulator that contributes to bacter...

show abstract

Section: Discussionmentioning

confidence: 99%

In Vitro Effects of Polyphosphate against Prevotella intermedia in Planktonic Phase and Biofilm

Jang

Kim

Noh

et al. 2016

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…Human hemoglobin (hHgb) is found in crevicular £uid of disease sites [9] and conceivably could be a source of iron for P. intermedia. Further, many P. intermedia strains also possess hemolytic activity that can liberate Hgb from erythrocytes [10]. P. intermedia strain 17, which possesses strong hemagglutinating and hemolytic activity [5,6,10], was used to determine if hHgb will support the growth of P. intermedia in vitro in the absence of other sources of iron and if so, to determine if iron uptake from hemoglobin involved initial binding of hHgb to P. intermedia.…”

Section: Introductionmentioning

confidence: 99%

The binding and utilization of hemoglobin by Prevotella intermedia

Leung

1998

FEMS Microbiology Letters

View full text Add to dashboard Cite

Prevotella intermedia, a putative periodontopathic microorganism, requires iron for growth. Hemoglobin can be a major source of iron for bacterial growth in vivo since it is present in the crevicular fluid collected from periodontitis sites. Experiments studying the growth of P. intermedia in iron-depleted Todd-Hewitt broth supplemented with human hemoglobin showed that the bacteria were able to utilize human hemoglobin as a source of iron. The uptake of iron from hemoglobin by P. intermedia appears to be initiated by the binding of hemoglobin to the bacteria as shown by direct binding studies using IPS Ilabeled human hemoglobin. Scatchard analysis of saturation binding data revealed that IPS I-labeled human hemoglobin had a dissociation constant (K d ) of 2.53U103V M for the receptor on P. intermedia. Binding of labeled hemoglobin to P. intermedia was competitively inhibited by unlabeled human hemoglobin showing that the binding was specific. The ability of bovine hemoglobin, but not hemin or non-hemoglobin heme-containing compounds, to inhibit binding competitively suggested that the globin moiety of the hemoglobin molecule is recognized by the hemoglobin binding receptors. z 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

show abstract

“…per ml in an anaerobic chamber (Vacuum Atmospheres, Hawthorne, Calif.) with an atmosphere of 5% CO 2 , 10% H 2 , and 85% N 2 as described previously (1,6). Escherichia coli MC1061 was grown in Luria-Bertani medium consisting of Bacto tryptone (10 g/liter), Bacto yeast extract (5 g/liter), and NaCl (10 g/liter) under aerobic conditions (6).…”

mentioning

confidence: 99%

Prevotella intermedia Stimulates Expansion of Vβ-Specific CD4 ⁺ T Cells

Leung

Torres

2000

Infect Immun

Self Cite

View full text Add to dashboard Cite

Recent evidence suggests that certain periodontal pathogens preferentially stimulate T cells expressing specific variable regions on the ␤ chain (V␤) of the T-cell receptor, which may indicate the presence of a superantigen. Superantigens are microbial proteins that activate large numbers of CD4 ؉ T cells in a V␤-specific manner. The purpose of this study was to determine whether Prevotella intermedia, a putative periodontal pathogen, activates populations of specific V␤ on CD4 ؉ T cells. Among the bacterial strains tested, P. intermedia strain 17, a clinical isolate, induced the strongest proliferative response in peripheral blood mononuclear cells. Antibodies raised against whole cells of this organism blocked the proliferative activity. P. intermedia-induced proliferation was T-cell specific and required the presence of antigen-presenting cells. Flow cytometric analysis showed that CD4 ؉ T-cell subsets expressing V␤8, V␤12, and V␤17 expanded in response to P. intermedia strain 17. The ability of P. intermedia to stimulate CD4؉ -T-cell proliferation was further supported by the production profiles of key T-cell cytokines, gamma interferon and interleukin-2. The data collectively suggest that certain strains of P. intermedia can activate V␤-specific T cells in a manner similar to that of other known microbial superantigens.

show abstract

Identification of hemolytic activity in Prevotella intermedia

Cited by 36 publications

References 44 publications

In Vitro Effects of Polyphosphate against Prevotella intermedia in Planktonic Phase and Biofilm

In Vitro Effects of Polyphosphate against Prevotella intermedia in Planktonic Phase and Biofilm

The binding and utilization of hemoglobin by Prevotella intermedia

Prevotella intermedia Stimulates Expansion of Vβ-Specific CD4 ⁺ T Cells

Contact Info

Product

Resources

About

Identification of hemolytic activity in Prevotella intermedia

Cited by 36 publications

References 44 publications

In Vitro Effects of Polyphosphate against Prevotella intermedia in Planktonic Phase and Biofilm

In Vitro Effects of Polyphosphate against Prevotella intermedia in Planktonic Phase and Biofilm

The binding and utilization of hemoglobin by Prevotella intermedia

Prevotella intermedia Stimulates Expansion of Vβ-Specific CD4 + T Cells

Contact Info

Product

Resources

About

Prevotella intermedia Stimulates Expansion of Vβ-Specific CD4 ⁺ T Cells