Identification of hemolytic activity and hemolytic genes of Methicillin-resistant Staphylococcus aureus isolated from Chinese children

Wang, Limin; Yang, Xin; Qian, Suyun; Liu, Yingchao; Yao, Kaihu; Dong, Fang; Song, Wenqi

doi:10.1097/cm9.0000000000000571

Cited by 7 publications

(5 citation statements)

References 5 publications

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“…According to Wang et al, S . aureus exhibits various hemolytic activities that can damage host cell membranes [ 51 ]. In our study, the positive control was S. aureus ATCC 6538 correspondingly growing on TSA.…”

Section: Methodsmentioning

confidence: 99%

Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (Apis mellifera L.)—An In Vitro Study

Leska

Nowak

Rosicka‐Kaczmarek

et al. 2023

Animals

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Lactic acid bacteria (LAB) are widely used probiotics and offer promising prospects for increasing the viability of honeybees. Thus, the probiotic potential of 10 LAB strains was determined, which in our previous studies showed the most potent protective abilities. In the current study, we investigated various properties of probiotic candidates. The tested LAB strains varied in susceptibility to tested antibiotics. Isolates showed high viability in sugar syrups and gastrointestinal conditions. None of the LAB strains exhibited β-hemolytic activity, mutual antagonism, mucin degradation, hydrogen peroxide production capacity, or bile salt hydrolase (BSH) activity. Additionally, the cytotoxicity of LAB cell-free supernatants (CFS) was assessed, as well as the effect of CFS from P. pentosaceus 14/1 on the cytotoxicity of coumaphos and chlorpyrifos in the Caco-2 cell line. The viability of Caco-2 cells reached up to 89.81% in the presence of the highest concentration of CFS. Furthermore, LAB metabolites decreased the cytotoxicity of insecticides (up to 19.32%) thus demonstrating cytoprotective activity. All tested LAB strains produced lactic, acetic, and malonic acids. This research allowed the selection of the most effective LAB strains, in terms of probiosis, for future in vivo studies aimed at developing an ecologically protective biopreparation for honeybees.

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Section: Methodsmentioning

confidence: 99%

Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (Apis mellifera L.)—An In Vitro Study

Leska

Nowak

Rosicka‐Kaczmarek

et al. 2023

Animals

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“…This enzyme is poisonous to various cells; leukocytes, erythrocytes, macrophages, and fibroblasts. This toxin most possibly undermined the plasma membrane and led to regularity in plasma membrane liquidity [33,34].…”

Section: Staphylococcal Toxinsmentioning

confidence: 99%

Staphylococcus Aureus Toxins and its Pathogenesis: A Review

Hashem Shehab,

MH Al-Shimmary,

Thanoon Ahmed

2023

ANJS

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Staphylococcus aureusis a grave community-acquired and nosocomial pathogen related to elevated levels of morbidity and mortality and produces a high number of toxins and other virulence agents. So, our review focuses on how S. aureusbegins, colonizes, and causes the infection and uponthe main determinants involved. This bacterium toxins included different types like (Alpha, Beta, Gamma, and Delta) Hemolysins, Leukotoxins, Staphylococcal Enterotoxins (SEs), Exfoliate Toxins (ETs) ,Toxic-Shock Syndrome Toxin (TSST)and Toxin-antitoxin (TA) systems.. Studying the determinants of staphylococcal virulence and determining its structure, function, and epidemiology will facilitate the development of strategies to eliminate its resistance to antibiotics, especially in the absence ofan anti-S. aureusvaccine.

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“…We recently reported on the hemolytic patterns (including hemolytic activity and hemolytic genes) of methicillin-resistant S. aureus (MRSA) isolates from Beijing Children' hospital (BCH) (Wang et al 2020). Interestingly, most clinical Clonal Complex 59 (CC59) isolates from children were α hemolysis negative.…”

Section: Introductionmentioning

confidence: 99%

“…So far, researchers have believed that S.aureus virulence is mainly associated with the production of Hlα and α hemolysis is a characteristic phenotype of S. aureus. However, we recently discovered α hemolysis negative CC59 isolates from Chinese children expressing β hemolysis instead (Wang et al 2020). In this study, we further compared the hemolytic activity of different clones of MRSA isolates from BCH, trying to nd the possible reason for α hemolytic deletion in CC59 isolates.…”

mentioning

confidence: 99%

The α-hemolysis negative CC59 clone of Methicillin-resistant Staphylococcus aureus isolates from Beijing，China

Wang

Sun

Qian

et al. 2022

Preprint

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Staphylococcus aureus is one of the most famous bacterial pathogens, it is a leading causative agent in skin and invasive infections. We analyzed the hemolytic patterns of MRSA isolates from Beijing Children’ hospital and found that most CC59 isolates were α hemolysis negative. In this study, we tried to find the possible reason for α hemolytic deletion in CC59 isolates. Hemolysis patterns of different MRSA types were showed. We have knocked out hlα gene of R23 isolate to form R23Δhlα isolate. A differential expression of hlα gene was examined among isolates with different hemolysis phenotypes using RT-PCR. We have examined the secretion of Hlα of R23, USA300 and R23Δhlα by Western blot. A high-copy-number plasmid containing the hlα gene was utilized to restore expression of α hemolysin.During the four periods (2009-2011, 2012-2013, 2016, 2017), 290 MRSA isolates were collected in Beijing Children’s Hospital (BCH). Most of the ST59 isolates (94.38%), and nine of the ST338 isolates (100%) showed negative α hemolysis phenotype. Both of them belong to CC30 clone. Transcriptional levels of hlα were equal among isolates showing α, δ hemolysis and β, δ hemolysis. R23 did not show α hemolysis phenotype but it produced Hlα. High-copy-number plasmid containing the hlα gene could restore α hemolysis phenotype of R23Δhlα isolate.This study elucidated that the negative α hemolysis in CC59 isolates was not related to Hlα protein production. However, over-production of Hlα could restore the α hemolytic activity. β hemolysin worked well in CC59 clone.

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Identification of hemolytic activity and hemolytic genes of Methicillin-resistant Staphylococcus aureus isolated from Chinese children

Cited by 7 publications

References 5 publications

Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (Apis mellifera L.)—An In Vitro Study

Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (Apis mellifera L.)—An In Vitro Study

Staphylococcus Aureus Toxins and its Pathogenesis: A Review

The α-hemolysis negative CC59 clone of Methicillin-resistant Staphylococcus aureus isolates from Beijing，China

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