Identification of gut‐derived metabolites of maslinic acid, a bioactive compound from Olea europaea L

Abstract

Maslinic acid undergoes phase I reactions resulting in a majority of monohydroxylated metabolites without the presence of phase II derivatives. The high concentration of maslinic acid achieved in the intestine suggests that it could exert a beneficial effect in the prevention of colon cancer.

Reduction of Preneoplastic Lesions Induced by 1,2-Dimethylhydrazine in Rat Colon by Maslinic Acid, a Pentacyclic Triterpene from Olea europaea L.

“…Maslinic acid was provided by Dr. Parra from the University of Granada (Granada, Spain). The triterpene was obtained as a pure (>95%) white powder after extraction of the olive pomace with ethyl acetate and further purification by two-step flash chromatography [14]. Betulinic acid, which was used as internal standard (I.S.…”

“…Rats had free access to both water and food (2014 Teklad Global 14%, Harlan, Barcelona, Spain). Maslinic acid was not detected in the commercial diet analyzed following an extraction method previously described [14]. Animal manipulation was performed in the morning to avoid the effects of circadian rhythms.…”

“…The concentration of maslinic acid in colon content was determined by liquid extraction prior to LC-APCI-MS analysis as previously described [14]. The quantification was carried out in the groups that were induced preneoplastic lesions and received maslinic acid at 0, 5, 10 and 25 mg/kg.…”

“…Among them, it its chemopreventive activity in the human colon adenocarcinoma cell lines HT29 and Caco-2 cells [11,12,13] is worth mentioning. Moreover, it was also demonstrated that after the oral administration of maslinic acid, high concentrations were found in the colon due to its low bioavailability [14]. Taking together these results, they suggest that the distal part of the intestine could be a target organ where maslinic acid can exert its beneficial effects.…”

Use of olive pomace extracts in broiler chicken diets: effects on growth performance and gut health

“…In this context, bioactive compounds of olive pomace such as oleuropein and hydroxytyrosol are good candidates to modulate the composition of gut microbiota and enhance gut integrity (Sarica and Ürmez, 2016). It is known that triterpenes offered through the diet are only partially absorbed in the upper digestive tract (Yin et al, 2012) and are also present along the GIT tract (Lozano-Mena et al, 2016).…”