Identification of gonadal soma-derived factor involvement in Monopterus albus (protogynous rice field eel) sex change

Zhu, Yefei; Wang, Chunlei; Chen, Xiaowu; Guan, Guijun

doi:10.1007/s11033-016-3997-8

Cited by 36 publications

(20 citation statements)

References 43 publications

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“…Although we do not know the function of Gsdf in salmon testis, it is likely that this protein has one or more roles as described in other fish species. One possible role may be associated with testis maturation, since we observed that gsdf expression was higher in immature compared to mature testis, similar to what has been observed in medaka, Japanese flounder, wrasse (Halichoeres trimaculatus) and rice field eel (Monopterus albus) [43,48,54,55]. The fact that we detected a 3.5fold lower number of gsdf reads in testis tissue devoid of germ cells compared to intact testis tissue may suggest a role for gsdf in the communication between germ cells and testicular somatic cells in Atlantic salmon.…”

Section: Gsdfsupporting

confidence: 86%

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

et al. 2020

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Background: Sustainability challenges are currently hampering an increase in salmon production. Using sterile salmon can solve problems with precocious puberty and genetic introgression from farmed escapees to wild populations. Recently sterile salmon was produced by knocking out the germ cell-specific dead end (dnd). Several approaches may be applied to inhibit Dnd function, including gene knockout, knockdown or immunization. Since it is challenging to develop a successful treatment against a gene product already existing in the body, alternative targets are being explored. Germ cells are surrounded by, and dependent on, gonadal somatic cells. Targeting genes essential for the survival of gonadal somatic cells may be good alternative targets for sterility treatments. Our aim was to identify and characterize novel germ cell and gonadal somatic factors in Atlantic salmon. Results: We have for the first time analysed RNA-sequencing data from germ cell-free (GCF)/dnd knockout and wild type (WT) salmon testis and searched for genes preferentially expressed in either germ cells or gonadal somatic cells. To exclude genes with extra-gonadal expression, our dataset was merged with available multi-tissue transcriptome data. We identified 389 gonad specific genes, of which 194 were preferentially expressed within germ cells, and 11 were confined to gonadal somatic cells. Interestingly, 5 of the 11 gonadal somatic transcripts represented genes encoding secreted TGF-β factors; gsdf, inha, nodal and two bmp6-like genes, all representative vaccine targets. Of these, gsdf and inha had the highest transcript levels. Expression of gsdf and inha was further confirmed to be gonad specific, and their spatial expression was restricted to granulosa and Sertoli cells of the ovary and testis, respectively. Finally, we show that inha expression increases with puberty in both ovary and testis tissue, while gsdf expression does not change or decreases during puberty in ovary and testis tissue, respectively. Conclusions: This study contributes with transcriptome data on salmon testis tissue with and without germ cells. We provide a list of novel and known germ cell-and gonad somatic specific transcripts, and show that the expression of two highly active gonadal somatic secreted TGF-β factors, gsdf and inha, are located within granulosa and Sertoli cells.

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Section: Gsdfsupporting

confidence: 86%

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

et al. 2020

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“…In O. luzonensis and sablefish, sex‐specific gsdf variants are present in genetic males and females (Myosho et al, ; Rondeau et al, ), and in Nile tilapia, chromosomally XX fish that are homozygous gsdf mutants reverse their sex to develop as males (Jiang et al, ). In addition, in two fish (three‐spot wrasse and rice field eel) that are sequential protogynous hermaphrodites, gsdf expression is up‐regulated as ovaries transform to testes (Horiguchi et al, ; Zhu et al, ). Although gsdf is not the primary genetic sex determinant in O. latipes (Japanese medaka), forced expression of gsdf on an autosome causes XX females to become males (Zhang et al, ) and gsdf knockout causes XY O. latipes to become phenotypic females, demonstrating that gsdf is strongly male determining in this species (Imai et al, ; Zhang et al, ).…”

Section: Discussionmentioning

confidence: 99%

Gonadal soma controls ovarian follicle proliferation through Gsdf in zebrafish

Yan

Desvignes

BreMiller

et al. 2017

Developmental Dynamics

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Background Aberrant signaling between germ cells and somatic cells can lead to reproductive disease and depends on diffusible signals, including TGFB-family proteins. The TGFB-family protein Gsdf (gonadal soma derived factor) controls sex determination in some fish and is a candidate for mediating germ cell/soma signaling. Results Zebrafish expressed gsdf in somatic cells of bipotential gonads and expression continued in ovarian granulosa cells and testicular Sertoli cells. Homozygous gsdf knockout mutants delayed leaving the bipotential gonad state, but then became a male or a female. Mutant females ovulated a few oocytes, then became sterile, accumulating immature follicles. Female mutants stored excess lipid and down-regulated aromatase, gata4, insulin receptor, estrogen receptor, and genes for lipid metabolism, vitellogenin, and steroid biosynthesis. Mutant females contained less estrogen and more androgen than wild types. Mutant males were fertile. Genomic analysis suggests that Gsdf, Bmp15, and Gdf9, originated as paralogs in vertebrate genome duplication events. Conclusions In zebrafish, gsdf regulates ovarian follicle maturation and expression of genes for steroid biosynthesis, obesity, diabetes, and female fertility, leading to ovarian and extra-ovarian phenotypes that mimic human polycystic ovarian syndrome (PCOS), suggesting a role for a related TGFB signaling molecule in the etiology of PCOS.

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“…Dmrt1 is generally considered to take charge of testicular differentiation cascade and could down-regulate cyp19a1a in vertebrates (Guiguen, Fostier, Piferrer, & Chang, 2010). Zhu, Wang, Chen, and Guan (2016) found that dmrt1 was absent in the ovary but present in Sertoli and gonia cells in testis and ovotestis of Monopterus albus, implying that it may be a essential regulatory factor for the transition of ovary to testis. The preliminary effects of sf-1, dax1 and amh on sex-inversion regulation have been demonstrated as well.…”

Section: Reproduction-related Genesmentioning

confidence: 99%

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Yang¹,

Lin²,

Li³

et al. 2019

Turk. J. Fish. Aquat. Sci.

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Amphiprion ocellaris is suggested to be an excellent research model for understanding socially controlled sex change of sequential hermaphrodite teleosts. However, there is a lack of knowledge regarding the genes involved in reproduction regulation in sex transition process. In order to enrich genetic data and sequence resources, Illumina HiSeq transcriptome sequencing was conducted for fully mature A. ocellaris using a multiple tissues mixing strategy. A total of 109,408,556 raw reads were obtained and 81,395 unigenes were generated by Trinity de novo assembly. Of which, 60,457 unigenes were annotated in at least one of the queried NR, COG, Swiss-Prot, KEGG and NT databases. After screening from reproduction-related Gene Ontology (GO) terms and pathways, plentiful candidate genes involved in reproductive process were identified, such as gnrh1, fsh, mtnr1a, avt, dmrt1, sox9a, amh, dax1, sf-1, foxl2 and cyp19a1. These candidates could make good starting points for profound mechanical analysis on the reproductive regulation during sex change. Moreover, 18,037 potential simple sequence repeats (SSRs) were detected in 14,212 unigenes for marker development. This transcript dataset will greatly increase available resources for gene mining and function analysis, and could contribute valuable information to in-depth studies on reproductive biology, genetics and genomics in the future.

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Identification of gonadal soma-derived factor involvement in Monopterus albus (protogynous rice field eel) sex change

Cited by 36 publications

References 43 publications

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

Gonadal soma controls ovarian follicle proliferation through Gsdf in zebrafish

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