Identification of glycosyltransferases involved in cell wall synthesis of wheat endosperm

Suliman, Muhtadi; Chateigner‐Boutin, Anne‐Laure; Francin-Allami, Mathilde; Partier, Anne; Bouchet, Brigitte; Salse, Jérôme; Pont, Caroline; Marion, Jessica; Rogniaux, Hélène; Tessier, Dominique; Guillon, Fabienne; Larré, Colette

doi:10.1016/j.jprot.2012.10.021

Cited by 21 publications

(29 citation statements)

References 70 publications

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“…Chromatographic separation was conducted on a reverse-phase capillary column (AcclaimPepmap C18 2 μm, 100 Å, 75 μm id × 15 cm in length, Thermo-Fisher Scientific) at a flow rate of 300 nL/min, as previously described [26]. The MS data acquisitions were performed using the Xcalibur 2.1 software.…”

Section: Methodsmentioning

confidence: 99%

Understanding the Remodelling of Cell Walls during Brachypodium distachyon Grain Development through a Sub-Cellular Quantitative Proteomic Approach

et al. 2016

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Brachypodium distachyon is a suitable plant model for studying temperate cereal crops, such as wheat, barley or rice, and helpful in the study of the grain cell wall. Indeed, the most abundant hemicelluloses that are in the B. distachyon cell wall of grain are (1-3)(1-4)-β-glucans and arabinoxylans, in a ratio similar to those of cereals such as barley or oat. Conversely, these cell walls contain few pectins and xyloglucans. Cell walls play an important role in grain physiology. The modifications of cell wall polysaccharides that occur during grain development and filling are key in the determination of the size and weight of the cereal grains. The mechanisms required for cell wall assembly and remodelling are poorly understood, especially in cereals. To provide a better understanding of these processes, we purified the cell wall at three developmental stages of the B. distachyon grain. The proteins were then extracted, and a quantitative and comparative LC-MS/MS analysis was performed to investigate the protein profile changes during grain development. Over 466 cell wall proteins (CWPs) were identified and classified according to their predicted functions. This work highlights the different proteome profiles that we could relate to the main phases of grain development and to the reorganization of cell wall polysaccharides that occurs during these different developmental stages. These results provide a good springboard to pursue functional validation to better understand the role of CWPs in the assembly and remodelling of the grain cell wall of cereals.

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Section: Methodsmentioning

confidence: 99%

Understanding the Remodelling of Cell Walls during Brachypodium distachyon Grain Development through a Sub-Cellular Quantitative Proteomic Approach

et al. 2016

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“…It is the most highly expressed CSLF gene in most tissues of barley (Hordeum vulgare), wheat (Triticum aestivum), Brachypodium distachyon, and rice (Oryza sativa), including developing seedling leaf, coleoptiles, and endosperm (Burton et al, 2008;Kimpara et al, 2008;Doblin et al, 2009;Nemeth et al, 2010;Pellny et al, 2012;Vega-Sánchez et al, 2012;Suliman et al, 2013;Trafford et al, 2013;Schreiber et al, 2014). When CSLF6 expression is reduced either by knockdown or knockout via mutation or T-DNA insertion (Tonooka et al, 2009;Nemeth et al, 2010;Taketa et al, 2012;Vega-Sánchez et al, 2012;Hu et al, 2014), a significant reduction in MLG is observed in both vegetative and floral tissues, indicating its gene product is responsible for the synthesis of the majority of MLG in grasses.…”

Section: Introductionmentioning

confidence: 99%

Determining the Subcellular Location of Synthesis and Assembly of the Cell Wall Polysaccharide (1,3; 1,4)-β-d-Glucan in Grasses

Wilson

Lampugnani

et al. 2015

The Plant Cell

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The current dogma for cell wall polysaccharide biosynthesis is that cellulose (and callose) is synthesized at the plasma membrane (PM), whereas matrix phase polysaccharides are assembled in the Golgi apparatus. We provide evidence that (1,3;1,4)-b-D-glucan (mixed-linkage glucan [MLG]) does not conform to this paradigm. We show in various grass (Poaceae) species that MLG-specific antibody labeling is present in the wall but absent over Golgi, suggesting it is assembled at the PM. Antibodies to the MLG synthases, cellulose synthase-like F6 (CSLF6) and CSLH1, located CSLF6 to the endoplasmic reticulum, Golgi, secretory vesicles, and the PM and CSLH1 to the same locations apart from the PM. This pattern was recreated upon expression of VENUS-tagged barley (Hordeum vulgare) CSLF6 and CSLH1 in Nicotiana benthamiana leaves and, consistent with our biochemical analyses of native grass tissues, shown to be catalytically active with CSLF6 and CSLH1 in PM-enriched and PM-depleted membrane fractions, respectively. These data support a PM location for the synthesis of MLG by CSLF6, the predominant enzymatically active isoform. A model is proposed to guide future experimental approaches to dissect the molecular mechanism(s) of MLG assembly.

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“…Maize (Galuszka et al, 2005) Aleurone Arabinoxylan Wheat (Beaugrand et al, 2005;Beaugrand, Reis, et al, 2004;Guillon et al, 2004;Lovegrove et al, 2013;Philippe et al, 2006;Philippe et al, 2007;Robert et al, 2011;Suliman et al, 2013); oat, rye ; barley Xylan Wheat (Lovegrove et al, 2013) Xyloglucan Barley (13),(14)--D-glucan Wheat Philippe et al, 2006;Robert et al, 2011;; barley Callose Wheat (Philippe et al, 2006); barley (Wilson et al, 2012) Mannan Barley Ferulic acid Wheat (Philippe et al, 2007;Robert et al, 2011) p-Coumaric acid Wheat Tranquet et al, 2009) Xylanase Wheat (Beaugrand, Cronier, et al, 2004;Beaugrand et al, 2005;Beaugrand, Reis, et al, 2004) Glycosyltransferase Wheat (Suliman et al, 2013) Oxalate oxidase Wheat ( Wheat (Van Herpen et al, 2008;Wiley et al, 2007) Oleosin / Caleosin Rice (Chen et al, 2012); oat (Heneen et al, 2008) Puroindolines Wheat (Capparelli et al, 2005) Transfer cells Arabinoxylan Wheat Lovegrove et al, 2013;Robert et al, 2011); oat, rye, barley …”

Section: Cytokinin Dehydrogenasementioning

confidence: 99%

“…Guillon, et al (2004) generated and characterized monoclonal and polyclonal antibodies against AX and were able to detect variations in structural features of AX, such as the degree of xylose substitution by arabinose within the wheat grain according to cell type. The same antibodies have helped to provide a better understanding of the AX biosynthesis process (Philippe, et al, 2006;Suliman, et al, 2013) and the role of AX substitution in the regulation of water movement within the endosperm . Two additional monoclonal antibodies against different epitopes of the xylan backbone, named LM10 and LM11, have been developed recently.…”

Section: Starchy Endosperm Arabinoxylanmentioning

confidence: 99%

“…The metabolism of cell wall polysaccharides requires the involvement of numerous enzymes. Suliman, et al (2013) recently identified glycosyltransferases involved in wheat endosperm cell wall formation. Immunogold labeling and silver enhancement were used in their study in order to localize AX and a polypeptide associated to the Golgi apparatus using TEM analyses.…”

Section: Grain Proteinsmentioning

confidence: 99%

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Current potential and limitations of immunolabeling in cereal grain research

Vázquez-Gutiérrez

Langton

2015

Trends in Food Science & Technology

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Immunolabeling techniques have made a valuable contribution to cereal grain research during the past decade in terms of precise localization of specific compounds. While these techniques have several limitations, such as the availability and specificity of the antibodies, immunolabeling has proven especially useful in cereal studies seeking a better understanding of grain development and characterization. According to the literature reviewed in this paper, immunolabeling techniques will continue to be a useful tool in the characterization and localization of cereal grain components.

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Identification of glycosyltransferases involved in cell wall synthesis of wheat endosperm

Cited by 21 publications

References 70 publications

Understanding the Remodelling of Cell Walls during Brachypodium distachyon Grain Development through a Sub-Cellular Quantitative Proteomic Approach

Understanding the Remodelling of Cell Walls during Brachypodium distachyon Grain Development through a Sub-Cellular Quantitative Proteomic Approach

Determining the Subcellular Location of Synthesis and Assembly of the Cell Wall Polysaccharide (1,3; 1,4)-β-d-Glucan in Grasses

Current potential and limitations of immunolabeling in cereal grain research

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