Identification of Glycogen in Whole Bacterial Cells by Infrared Spectrophotometry

Levine, Seymour; Stevenson, Heber J. R.; Bordner, Robert H.

doi:10.1126/science.118.3057.141

Cited by 25 publications

(12 citation statements)

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“…2B) reveal that the positive feature, which is present only in F3;16, could arise from the vibrations of the C@O near 1738 cm À1 in PHAs [26] and the C@O near 1712 cm À1 in FFA [27]. In contrast, the negative features near 1157 cm À1 and 1022 cm À1 arise from the vibrations of glycosidic bonds and the CAOAC and CAOAP bonds of glycogen [28,29] (Table S1). A close comparative examination of the cluster vector plots shows that the overall polysaccharide features in all engineered strains decreased relative to the F0.…”

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confidence: 96%

Metabolic phenotyping of the cyanobacterium Synechocystis 6803 engineered for production of alkanes and free fatty acids

Borglin

Kamennaya

et al. 2013

Applied Energy

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confidence: 96%

Metabolic phenotyping of the cyanobacterium Synechocystis 6803 engineered for production of alkanes and free fatty acids

Borglin

Kamennaya

et al. 2013

Applied Energy

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“…Glycogen is a common reserve material among eukaryotes and eubacteria [1][2][3][4][5][6][7][8][9] and it was also found in thermoacidophilic archaebacteria [10].…”

Section: Introductionmentioning

confidence: 99%

Glycogen in Methanolobus and Methanococcus

König

1985

FEMS Microbiology Letters

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Glycogen was isolated from Methanolobus tindarius and 4 species of Methanococcus. It was identified by the iodine reaction, determination of glucose, maltose and isomaltose in acid hydrolysates and partial acid hydrolysates, respectively, determination of erythritol and glycerol after periodate oxidation and treatment with amyloglucosidase. Glycogen particles in the cells are distributed throughout the cytoplasm. Ml. tindarius can use elemental nitrogen and cysteine as nitrogen source. The glycogen and polyphosphate content of cells of Ml. tindarius increased under nitrogen limitation. During incubation without external energy source, glycogen decreased and methane formation was correlated to the glycogen content of the cells.

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“…LEVIN et al (9)(10)(11) in 1953 and 1955 studied chemical components of enteric bacteria and klebsiella and attempted to classify and identify these bacteria by their infrared absorption spectra. THOMAS and GREENSTREET (12) in 1954 compared the absorption spectra of closely related strains of pasteurella, escherichia and salmonella.…”

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confidence: 99%

“…The chemical interpretation of bacterial infrared spectra was made by LEVINE and coworkers (9,10), RIDEAL and ADAMS (20) and was described in the comprehensive reviews of CHIHARA (21) and NoRRIS (22). WAKSMAN and HENRICI (23) classified organisms belonging to the order Actinomycetales into three families: Mycobacteriaceae, Actinomycetaceae, and Streptomycetaceae.…”

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confidence: 99%