Identification of glutathione conjugates of 2,3-dibromopropene in male ICR mice

Lee, Sang Kyu; Baik, Seo Yeon; Jeon, Tae Won; Jun, In Hye; Kim, Ghee Hwan; Jin, Chun Hua; Lee, Dong Ju; Kim, Jun Kyou; Yum, Young Na; Jeong, Tae Cheon

doi:10.1007/bf02974280

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…The time-course study showed that depletion of GSH by production of S-bromopropyl GSH conjugate was parallel with hepatotoxicity induced by 1,3-DBP. In fact, the decreased level of hepatic GSH following treatment with 1-BP and 2,3-DBPE was also associated with increased hepatotoxicity in male ICR mice (Lee et al, 2005b;2006).…”

Section: S-mentioning

confidence: 91%

Hepatotoxic and Immunotoxic Effects produced by 1,3‐Dibromopropane and Its Conjugation with Glutathione in Female BALB/c Mice

Lee

Jeong

et al. 2007

Journal of Toxicology and Environmental Health, Part A

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To determine a possible role of glutathione (GSH) conjugation in 1,3-dibromopropane (1,3-DBP)-induced hepatotoxicity and immunotoxicity, female BALB/c mice were treated orally with 1,3-DBP. Based on the liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS) analyses, two forms of S-bromopropyl GSH were observed at m/z 427.9 and 429.9 in the positive ESI spectrum with a retention time of 5.29 and 5.23 min, respectively. Following single treatment of mice with 150, 300 or 600 mg/kg 1,3-DBP for 12 hr, the amount of S-bromopropyl GSH was detected maximally in liver homogenates at 600 mg/kg 1,3-DBP. Hepatic GSH levels were significantly decreased by treatment with 1,3-DBP. In a time course study, production of S-bromopropyl GSH rose maximally 6 hr after treatment and decreased gradually thereafter. The liver weights were significantly increased by treatment with 600 mg/kg 1,3-DBP. When mice were treated orally with 600 mg/kg 1,3-DBP for 12 hr, the activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were increased by 365- and 83-fold. In addition, oral 1,3-DBP significantly suppressed the antibody response to a T-dependent antigen at 600 mg/kg 1,3-DBP. 1,3-DBP elevated hepatic levels of malondialdehyde and suppressed the activities of some hepatic enzymes involved in anti-oxidation. Taken together, the formation of GSH conjugate with 1,3-DBP may deplete cellular GSH and, subsequently, produce hepatotoxicity and immunotoxicity via damage to the cellular anti-oxidative system.

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Section: S-mentioning

confidence: 91%

Hepatotoxic and Immunotoxic Effects produced by 1,3‐Dibromopropane and Its Conjugation with Glutathione in Female BALB/c Mice

Lee

Jeong

et al. 2007

Journal of Toxicology and Environmental Health, Part A

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“…: +82-53-810-2819; Fax: +82-53-810-4654; E-mail: taecheon@yumail.ac.kr tathione (GSH). [3][4][5][6][7] Some haloalkanes capable of forming GSH conjugates may cause toxicity related directly or possibly with GSH conjugation. For example, mice treated orally with 1-BP exhibit hepatotoxicity and immunotoxicity following depletion of GSH in liver and spleen.…”

Section: Introductionmentioning

confidence: 99%

Hepatotoxicity and Immunotoxicity of 1-Bromohexane and Its Glutathione Conjugation in Female BALB/c Mice

Lee

et al. 2010

JOURNAL OF HEALTH SCIENCE

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Hepatotoxic and immunotoxic effects of 1-bromohexane (1-BH) and its conjugation with glutathione (GSH) were investigated in female BALB/c mice. The animals were treated once orally with 1-BH at 500, 1000, and 2000 mg/kg in corn oil for a dose-response study or treated orally with 1-BH at 2000 mg/kg for 6, 12, 24, and 48 hr for a time-course study. Treatment with 1-BH increased serum activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) dose dependently. The hepatic contents of thiobarbituric acid reactive substances were significantly increased at 2000 mg/kg of 1-BH from 12 to 24 hr after the treatment. Oral 1-BH at 2000 mg/kg significantly suppressed production of splenic intracellular interleukin (IL)-2 in response to concanavalin A. Following treatment with 1-BH, three GSH conjugates such as S-hexyl GSH, S-hexyl cysteine, and hydroxyhexyl mercapturic acid were identified in livers by liquid chromatography-electrospray ionization tandem mass spectrometry. The hepatic contents of GSH were maximally decreased 6 hr after treatment with 1-BH. GSH conjugates were also detected maximally in livers 6 hr after treatment. These results suggest that 1-BH could cause hepatotoxicity and immunotoxicity as well as depletion of GSH content due to the formation of GSH conjugates with 1-BH in female BALB/c mice.

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Identification of glutathione conjugates of 2,3-dibromopropene in male ICR mice

Cited by 2 publications

References 13 publications

Hepatotoxic and Immunotoxic Effects produced by 1,3‐Dibromopropane and Its Conjugation with Glutathione in Female BALB/c Mice

Hepatotoxic and Immunotoxic Effects produced by 1,3‐Dibromopropane and Its Conjugation with Glutathione in Female BALB/c Mice

Hepatotoxicity and Immunotoxicity of 1-Bromohexane and Its Glutathione Conjugation in Female BALB/c Mice

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