1994
DOI: 10.1038/bjc.1994.402
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Identification of glioma-associated antigen MUC 2-63 as CD44

Abstract: SmryMonoclonal antibody MUC 2-63 recognses neuroenic tumours and has been used successfilly for radiimaging human malignant ghomas. We now show that the MUC 2-63 antigen has the same tissue distibution and molecular weght rae as the CD44 antigen and confirm the identity of these two molecule in bcking studies using MUC 2-63 and the CD44 anti-framework antibody FIO44-2. Thus not only MUC 2-63 but also other anti-CD44 monodcnal antibodies should prove useful in imaging and, perhaps, therapy of brain tumours.The … Show more

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Cited by 10 publications
(6 citation statements)
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“…The slides were treated in a microwave oven (750 W) twice for 6 min in 10 mM citrate buffer (pH 6.0) for demascation of the antigen followed by incubation in 0.3% H 2 O 2 in methanol. Then, 10% goat serum was applied for 30 min followed by MUC 2‐63 antibody directed against the standard form of CD44 at a concentration of 10 μg/ml for 60 min [ 21, 22]. Biotinylated secondary antibody (1:60, goat anti‐mouse, Sigma, Poole, UK) and peroxidase‐labelled streptavidin (1:100–1:200, Dako, Hamburg, Germany) were added in sequence.…”
Section: Methodsmentioning
confidence: 99%
“…The slides were treated in a microwave oven (750 W) twice for 6 min in 10 mM citrate buffer (pH 6.0) for demascation of the antigen followed by incubation in 0.3% H 2 O 2 in methanol. Then, 10% goat serum was applied for 30 min followed by MUC 2‐63 antibody directed against the standard form of CD44 at a concentration of 10 μg/ml for 60 min [ 21, 22]. Biotinylated secondary antibody (1:60, goat anti‐mouse, Sigma, Poole, UK) and peroxidase‐labelled streptavidin (1:100–1:200, Dako, Hamburg, Germany) were added in sequence.…”
Section: Methodsmentioning
confidence: 99%
“…Nevertheless, production of human antibodies, recombinant antigen-specific molecules like chimeric antibodies, single chain fragments of the variable region (scFv), and immunotoxins usually yield lower quantities. Various procaryontic and eucaryontic expression systems for production of fusion proteins are now available for different purposes ( 5 ) .…”
Section: Production Of Mabsmentioning
confidence: 99%
“…Since human mAbs are rare and human hybridomas are seldomly stable, one of the first antibody constructs consisted of the murine genes coding for the variable region domains combined with human constant region domain genes. The resulting chimeric mouse/human mAbs showed reduced immunogenicity and improved ADCC and CDC (5). Apossibility to further 'humanize.. mAbs consists of grafting of the framework regions.…”
Section: Optimizing Mabs For Application In Patientsmentioning
confidence: 99%
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“…Some members of the mucin family have been detected in gliomas (Hagel et al 1994; Romeijn et al 1994; Oosterkamp et al 1997). However, to date, MUC16 mutations have not yet been explored in these tumors.…”
Section: Introductionmentioning
confidence: 99%