Identification of genetic variations of exon 2 of <i>BoLA‐DQB</i> gene in five Chinese yellow cattle breeds

Wang, K.; Sun, Dongxiao; Zhang, Y.

doi:10.1111/j.1744-313x.2007.00654.x

Cited by 11 publications

(6 citation statements)

References 11 publications

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“…LCP1 , a leukocyte-specific F-actin bundling protein, is implicated in T lymphocyte polarity and migration in response to chemokines [45]. BLA-DQB is one of the genes of the major histocompatibility complex in cattle, a family of highly polymorphic genes that play a central role antigen presentation during adaptive immune responses [46]. Importantly, all these genes were decreased in the embryos from methionine-supplemented dams.…”

Section: Discussionmentioning

confidence: 99%

Effect of Maternal Methionine Supplementation on the Transcriptome of Bovine Preimplantation Embryos

et al. 2013

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Maternal nutrition exclusively during the periconceptional period can induce remarkable effects on both oocyte maturation and early embryo development, which in turn can have lifelong consequences. The objective of this study was to evaluate the effect of maternal methionine supplementation on the transcriptome of bovine preimplantation embryos. Holstein cows were randomly assigned to one of two treatments differing in level of dietary methionine (1.89 Met vs. 2.43 Met % of metabolizable protein) from calving until embryo flushing. High quality preimplantation embryos from individual cows were pooled and then analyzed by RNA sequencing. Remarkably, a subtle difference in methionine supplementation in maternal diet was sufficient to cause significant changes in the transcriptome of the embryos. A total of 276 genes out of 10,662 showed differential expression between treatments (FDR <0.10). Interestingly, several of the most significant genes are related to embryonic development (e.g., VIM, IFI6, BCL2A1, and TBX15) and immune response (e.g., NKG7, TYROBP, SLAMF7, LCP1, and BLA-DQB). Likewise, gene set enrichment analysis revealed that several Gene Ontology terms, InterPro entries, and KEGG pathways were enriched (FDR <0.05) with differentially expressed genes involved in embryo development and immune system. The expression of most genes was decreased by maternal methionine supplementation, consistent with reduced transcription of genes with increased methylation of specific genes by increased methionine. Overall, our findings provide evidence that supplementing methionine to dams prior to conception and during the preimplantation period can modulate gene expression in bovine blastocysts. The ramifications of the observed gene expression changes for subsequent development of the pregnancy and physiology of the offspring warrant further investigation in future studies.

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Section: Discussionmentioning

confidence: 99%

Effect of Maternal Methionine Supplementation on the Transcriptome of Bovine Preimplantation Embryos

et al. 2013

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“…The BoLA-DQB was typed using KOD plus PCR reaction kit (Toyobo, Tokyo, Japan) as described by Wang et al (2007). Amplicon purification, sequence processing, and data analysis were carried out as described for DRB3 typing.…”

Section: Bola-dqb Typingmentioning

confidence: 99%

“…Interpreting these studies has been difficult because the full-genome sequence and genomic organization of the BoLA region has only recently been fully established. Polymorphisms in cattle MHC genes have been relatively well studied but the majority of studies have focused on single loci, commonly the BoLA-DRB3 gene, and have given little attention to the multiple DQA and DQB genes (Mikko and Andersson, 1995;Ballingall et al, 1997Ballingall et al, , 1998Takeshima et al, 2002Takeshima et al, , 2003Takeshima et al, , 2009Takeshima et al, , 2008aWang et al, 2005Wang et al, , 2007. Furthermore, most of the reported BoLA class II DR-DQ-linked haplotypes have focused on Holstein cattle and have used a relatively small sample population (Lewin et al, 1999;Glass et al, 2000;Russell, 2000;Park et al, 2004;Norimine and Brown, 2005;Staska et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Identification and diversity of bovine major histocompatibility complex class II haplotypes in Japanese Black and Holstein cattle in Japan

Miyasaka

Takeshima

Sentsui

et al. 2012

Journal of Dairy Science

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Bovine leukocyte antigen (BoLA), the major histocompatibility complex of cattle, is one of the most polymorphic gene clusters. We genotyped a population of 109 Japanese Black and 39 Holstein cattle to analyze their BoLA class II haplotypes, BoLA-DRB3 locus, 5 BoLA-DQA loci, and 5 BoLA-DQB loci. We identified 26 previously reported DRB3 alleles, 22 previously reported and 3 new DQA alleles, and 24 previously reported and 6 new DQB alleles. A dendrogram was constructed based on the predicted amino acid sequences of the α1 or β1 domains encoded by BoLA-DQA or -DQB alleles, which revealed that DQA alleles were clustered into 5 loci, whereas DQB alleles could not be clearly assigned to specific DQB loci. The BoLA-DRB3-DQA-DQB haplotypes were sorted by sequential analytical processes, and 42 distinct haplotypes, including 11 previously published haplotypes and 31 novel haplotypes, were defined. Strong linkage disequilibrium was present in the BoLA genes. We also compared DRB3-DQA1 haplotype frequencies between 507 Japanese Black and 143 Holstein cattle. Thirty-nine DRB3-DQA1 haplotypes were identified, including 29 haplotypes from Japanese Black and 22 haplotypes from Holstein cattle. The majority of the haplotypes could be identified in both breeds, although several haplotypes were identified in only a single breed. This is the first report presenting a detailed study of the BoLA class II haplotype in Japanese Black and Holstein cattle in Japan.

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“…Polymorphism in cattle MHC genes has been relatively well studied. However, most studies have focused on a single locus, commonly the BoLA‐DRB3 gene, with little attention given to the multiple DQA and DQB genes . Furthermore, most of the reported BoLA class II DR‐DQ –linked haplotypes have been from Holstein cattle, using a relatively small sample population .…”

Section: Introductionmentioning

confidence: 99%

Identification of bovine leukocyte antigen class II haplotypes associated with variations in bovine leukemia virus proviral load in Japanese Black cattle

et al. 2012

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Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, which is the most common neoplastic disease of cattle. Bovine leukocyte antigen (BoLA) is strongly involved in the subclinical progression of BLV infections. Recent studies show that the BoLA-DRB3 gene might play a direct role in controlling the number of BLV-infected peripheral B lymphocytes in vivo in Holstein cattle. However, the specific BoLA class II allele and DRB3-DQA1 haplotypes determining the BLV proviral load in Japanese Black cattle are yet to be identified. In this study, we focused on the association of BLV proviral load and polymorphism of BoLA class II in Japanese Black cattle. We genotyped 186 BLV-infected, clinically normal cattle for BoLA-DRB3 and BoLA-DQA1 using a polymerase chain reaction-sequence-based typing method. BoLA-DRB3*0902 and BoLA-DRB3*1101 were associated with a low proviral load (LPVL), and BoLA-DRB3*1601 was associated with a high proviral load (HPVL). Furthermore, BoLA-DQA1*0204 and BoLA-DQA1*10012 were related to LPVL and HPVL, respectively. Furthermore, we confirmed the correlation between the DRB3-DQA1 haplotype and BLV proviral load. Two haplotypes, namely 0902B or C (DRB3*0902-DQA1*0204) and 1101A (DRB3*1101-DQA1*10011), were associated with a low BLV proviral load, whereas one haplotype 1601B (DRB3*1601-DQA1*10012) was associated with a high BLV proviral load. We conclude that resistance is a dominant trait and susceptibility is a recessive trait. Additionally, resistant alleles were common between Japanese Black and Holstein cattle, and susceptible alleles differed. This is the first report to identify an association between the DRB3-DQA1 haplotype and variations in BLV proviral load.

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Identification of genetic variations of exon 2 of BoLA‐DQB gene in five Chinese yellow cattle breeds

Cited by 11 publications

References 11 publications

Effect of Maternal Methionine Supplementation on the Transcriptome of Bovine Preimplantation Embryos

Effect of Maternal Methionine Supplementation on the Transcriptome of Bovine Preimplantation Embryos

Identification and diversity of bovine major histocompatibility complex class II haplotypes in Japanese Black and Holstein cattle in Japan

Identification of bovine leukocyte antigen class II haplotypes associated with variations in bovine leukemia virus proviral load in Japanese Black cattle

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