Identification of Genes with Nutrient-controlled Expression by PCR-mapping in the YeastSaccharomyces cerevisiae

Crauwels, Marion; Winderickx, Joris; Winde, Johannes H. de; Thevelein, Johan M.

doi:10.1002/(sici)1097-0061(199708)13:10<973::aid-yea146>3.0.co;2-s

Cited by 12 publications

(5 citation statements)

References 47 publications

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“…To study the response to glucose starvation, RNA was isolated 1 h after transferring cells of the four strains from SD to S medium. We analyzed two genes already characterized for this stress condition in yeast: HSP12 and SSA3 (Crauwels et al, 1997). Figure 4D shows that in the case of HSP12 all the strains showed high induction levels except LYCC 047, similar to what has been observed under other stress conditions.…”

Section: Stress Due To Glucose Starvationsupporting

confidence: 70%

An inverse correlation between stress resistance and stuck fermentations in wine yeasts. A molecular study

Ivorra

Pérez-Ortín

Olmo

1999

Biotechnol. Bioeng.

103

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During alcoholic fermentations yeast cells are subjected to several stress conditions and, therefore, yeasts have developed molecular mechanisms in order to resist this adverse situation. The mechanisms involved in stress response have been studied in Saccharomyces cerevisiae laboratory strains. However a better understanding of these mechanisms in wine yeasts could open the possibility to improve the fermentation process. In this work an analysis of the stress response in three wine yeasts has been carried out by studying the expression of several representative genes under several stress conditions which occur during fermentation. We propose a simplified method to study how these stress conditions affect the viability of yeast cells. Using this approach an inverse correlation between stress‐resistance and stuck fermentations has been found. We also have preliminary data about the use of the HSP12 gene as a molecular marker for stress‐resistance in wine yeasts. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 64: 698–708, 1999.

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Section: Stress Due To Glucose Starvationsupporting

confidence: 70%

An inverse correlation between stress resistance and stuck fermentations in wine yeasts. A molecular study

Ivorra

Pérez-Ortín

Olmo

1999

Biotechnol. Bioeng.

103

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“…Most recently, it was shown that Sch9 phosphorylates Maf1 at seven distinct sites in vitro and that it thereby can not only partially inhibit nuclear accumulation of Maf1 after rapamycin treatment, but also completely block the repressive association between Maf1 and the RNA Pol III subunit Rpc82 (Huber et al 2009). In addition to ribosome biogenesis, Sch9 controls the expression of different tRNA synthetases, proteins involved in amino acid metabolism as well as translation initiation and elongation factors (Crauwels et al 1997b;Roosen et al 2005) and recent evidence suggests that Sch9 also controls the phosphorylation status of eIF2a (Urban et al 2007). Hence, Sch9 functions as a central coordinator of protein synthesis and this may explain why sch9D cells are characterized by a small cell size, since, indeed, the translational capacity of a cell is tightly coupled to the size threshold at which cells commit to cell division (Jorgensen et al 2002(Jorgensen et al , 2004.…”

Section: The Protein Kinase Sch9mentioning

confidence: 99%

Life in the midst of scarcity: adaptations to nutrient availability in Saccharomyces cerevisiae

et al. 2010

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Cells of all living organisms contain complex signal transduction networks to ensure that a wide range of physiological properties are properly adapted to the environmental conditions. The fundamental concepts and individual building blocks of these signalling networks are generally well-conserved from yeast to man; yet, the central role that growth factors and hormones play in the regulation of signalling cascades in higher eukaryotes is executed by nutrients in yeast. Several nutrient-controlled pathways, which regulate cell growth and proliferation, metabolism and stress resistance, have been defined in yeast. These pathways are integrated into a signalling network, which ensures that yeast cells enter a quiescent, resting phase (G 0 ) to survive periods of nutrient scarceness and that they rapidly resume growth and cell proliferation when nutrient conditions become favourable again. A series of well-conserved nutrient-sensory protein kinases perform key roles in this signalling network: i.e. Snf1, PKA, Tor1 and Tor2, Sch9 and Pho85-Pho80. In this review, we provide a comprehensive overview on the current understanding of the signalling processes mediated via these kinases with a particular focus on how these individual pathways converge to signalling networks that ultimately ensure the dynamic translation of extracellular nutrient signals into appropriate physiological responses.

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“…Several modifications of the original technique have been reported with some solutions to the key problems identified by some authors [17]. Stress responses have been studied using DDRT-PCR in C. elegans and S. cerevisiae [18-20]. DDRT-PCR has been applied in many laboratories to identify genes involved in signal cascades.…”

Section: Introductionmentioning

confidence: 99%

Transient expression of βC1 protein differentially regulates host genes related to stress response, chloroplast and mitochondrial functions

Andleeb

Amin

Bashir

et al. 2010

Virol J

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BackgroundGeminiviruses are emerging plant pathogens that infect a wide variety of crops including cotton, cassava, vegetables, ornamental plants and cereals. The geminivirus disease complex consists of monopartite begomoviruses that require betasatellites for the expression of disease symptoms. These complexes are widespread throughout the Old World and cause economically important diseases on several crops. A single protein encoded by betasatellites, termed βC1, is a suppressor of gene silencing, inducer of disease symptoms and is possibly involved in virus movement. Studies of the interaction of βC1 with hosts can provide useful insight into virus-host interactions and aid in the development of novel control strategies. We have used the differential display technique to isolate host genes which are differentially regulated upon transient expression of the βC1 protein of chili leaf curl betasatellite (ChLCB) in Nicotiana tabacum.ResultsThrough differential display analysis, eight genes were isolated from Nicotiana tabacum, at two and four days after infitration with βC1 of ChLCB, expressed under the control of the Cauliflower mosaic virus 35S promoter. Cloning and sequence analysis of differentially amplified products suggested that these genes were involved in ATP synthesis, and acted as electron carriers for respiration and photosynthesis processes. These differentially expressed genes (DEGs) play an important role in plant growth and development, cell protection, defence processes, replication mechanisms and detoxification responses. Kegg orthology based annotation system analysis of these DEGs demonstrated that one of the genes, coding for polynucleotide nucleotidyl transferase, is involved in purine and pyrimidine metabolic pathways and is an RNA binding protein which is involved in RNA degradation.ConclusionβC1 differentially regulated genes are mostly involved in chloroplast and mitochondrial functions. βC1 also increases the expression of those genes which are involved in purine and pyrimidine metabolism. This information gives a new insight into the interaction of βC1 with the host and can be used to understand host-virus interactions in follow-up studies.

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Identification of Genes with Nutrient-controlled Expression by PCR-mapping in the YeastSaccharomyces cerevisiae

Cited by 12 publications

References 47 publications

An inverse correlation between stress resistance and stuck fermentations in wine yeasts. A molecular study

An inverse correlation between stress resistance and stuck fermentations in wine yeasts. A molecular study

Life in the midst of scarcity: adaptations to nutrient availability in Saccharomyces cerevisiae

Transient expression of βC1 protein differentially regulates host genes related to stress response, chloroplast and mitochondrial functions

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