Identification of genes whose transcripts accumulate rapidly in tomato after root-knot nematode infection

Lambert, Kris N.; Ferrie, Bonita J. M.; Nombela, Gloria; Brenner, Eric D.; Williamson, Valerie M.

doi:10.1006/pmpp.1999.0239

Cited by 46 publications

(35 citation statements)

References 29 publications

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“…The partial resistance of H-10-15 and Moisson could explain minor changes in IEF enzyme profiles found in some isozyme systems between infected and uninfected plants. Our conclusion is similar to those of Lambert et al (1999) with respect to the Mi gene in tomato; the products of some resistance genes with increased expression (isozyme activity) early after CCN infection could be involved in nematode resistance mediated by RG, and others could form part of a more-general defence system that is present in both resistant and susceptible wheat.…”

Section: Discussionsupporting

confidence: 81%

“…In plants with resistance to nematodes, esterases, peroxidases and phenylalanine ammonia lyases increased after nematode infection, while a reduced level of superoxide dismutase occurred Lambert et al 1999;AndrØs et al 2001). Early histological and biochemical responses of roots from resistant line H-93-8 to the Spanish pathotype Ha71 have previously been reported, showing a strong correlation between cytological expression of resistance (hypersensitive response) and peroxidase and esterase activity (AndrØs et al 2001).…”

Section: Introductionmentioning

confidence: 89%

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Biochemical and genetic studies of two Heterodera avenae resistance genes transferred from Aegilops ventricosa to wheat

Montes¹,

López-Braña²,

Balseiro-Romero

et al. 2003

Theor Appl Genet

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Two Heterodera avenae resistance genes, Cre2 from Aegilops ventricosa AP-1 and Cre5 from Ae. ventricosa #10, were shown to confer a high level of resistance to the Spanish pathotype Ha71. No susceptible plants were found in the F(2) progeny from the cross between the two accessions of Ae. ventricosa, suggesting that their respective resistance factors were allelic. However, genes Cre2 and Cre5 apparently were transferred to a different chromosomal location in the wheat line H-93-8 and in the 6M(v)(6D) substitution, respectively, as proved by F(2) segregation of their cross progeny. The induction of several defence responses during early infection by the same H. avenae pathotype in resistant lines carrying Cre2 or Cre5 genes was studied. Isoelectrofocusing (IEF) isozyme analysis revealed that peroxidase, esterase and superoxide dismutase activity increased after nematode infection, in roots of resistant lines in comparison with their susceptible parents. Differential induced isoforms were also identified when IEF patterns of resistant lines were compared. A DNA marker, absent in Cre5-carrying genotypes, was found to be linked, thought not very tightly, to the Cre2 gene in the H-93-8 line. The differences observed between the Cre2 and Cre5 genes with respect to their chromosomal location in wheat introgression lines, de-toxificant enzyme induction and behaviour against different pathotypes, suggest they are different H. avenae resistance sources for wheat breeding.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 89%

Biochemical and genetic studies of two Heterodera avenae resistance genes transferred from Aegilops ventricosa to wheat

Montes¹,

López-Braña²,

Balseiro-Romero

et al. 2003

Theor Appl Genet

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“…Genes encoding Leu-rich repeats, a protein motif common in resistance genes, are more regulated (both up and down) in susceptible reactions in Mixed than they are in the resistance comparison. The two genes encoding late embryogenesis abundant motifs are regulated in all experiment comparisons except in the resistance reaction, consistent with previous findings that such genes are globally up-regulated following RKN infection (van der Eycken et al, 1996;Lambert et al, 1999). Of the genes encoding domains identified as peroxidase motifs that were regulated in resistant roots, all were up-regulated.…”

Section: Comparison Of the Rkn-responsive Transcriptome Across Treatmsupporting

confidence: 90%

Comprehensive Transcriptome Profiling in Tomato Reveals a Role for Glycosyltransferase in Mi-Mediated Nematode Resistance

et al. 2007

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Root-knot nematode (RKN; Meloidogyne spp.) is a major crop pathogen worldwide. Effective resistance exists for a few plant species, including that conditioned by Mi in tomato (Solanum lycopersicum). We interrogated the root transcriptome of the resistant (Mi1) and susceptible (Mi-) cultivars 'Motelle' and 'Moneymaker,' respectively, during a time-course infection by the Mi-susceptible RKN species Meloidogyne incognita and the Mi-resistant species Meloidogyne hapla. In the absence of RKN infection, only a single significantly regulated gene, encoding a glycosyltransferase, was detected. However, RKN infection influenced the expression of broad suites of genes; more than half of the probes on the array identified differential gene regulation between infected and uninfected root tissue at some stage of RKN infection. We discovered 217 genes regulated during the time of RKN infection corresponding to establishment of feeding sites, and 58 genes that exhibited differential regulation in resistant roots compared to uninfected roots, including the glycosyltransferase. Using virus-induced gene silencing to silence the expression of this gene restored susceptibility to M. incognita in 'Motelle,' indicating that this gene is necessary for resistance to RKN. Collectively, our data provide a picture of global gene expression changes in roots during compatible and incompatible associations with RKN, and point to candidates for further investigation.

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“…A plant gene conferring resistance to M. incognita, called Mi, was first isolated from tomato by positional cloning (Milligan et al 1998), and encoded an NBS-LRR type protein. Other genes associated with response to nematode infection have been isolated by several researchers in different species (Lambert et al 1999;Potenza et al 2001). More recently, M. arenaria-challenged resistant species A. stenosperma Morgante et al 2011) identified many responsive genes.…”

Section: Etiologymentioning

confidence: 99%

Marker‐Assisted Selection for Biotic Stress Resistance in Peanut

Burow¹,

Leal‐Bertioli²,

Simpson³

et al. 2013

Translational Genomics for Crop Breeding

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Peanut is the second-most important legum e grown worldwide. Cultivated peanut is a disomic tetraploid, 2n-4 x -40, with limited genetic diversity due to a genetic bottleneck in formation o f the polyploid from ancestors A. duranensis and A. ipaensis. Consequently, resistance_to biotic stresses is limited in the cultigen; however, w ild species possess strong resistances. Transfer o f these re sistances is hindered by differences o f ploidy, but production o f synthetic amphidiploids, coupled with use o f m olecular markers, enables efficient gene transfer. Marker maps have been made from interspecific crosses, and SSR-based maps from cultivated parents have been developed recently. At least 410 resistance gene analogues have been identified. The first markers for biotic stress tolerance were for root-knot nematode resistance and introgressed from one A. cardenasii chromosome. These and improved markers have been used for marker-assisted backcrossing, contributing to release o f three cultivars. Additional QTLs have been identified since.Early and late leafspots cause significant yield losses worldwide, and resistance depends on multiple genes. U sing interspecific populations, five resistance QTLs for early leafspot were identified using greenhouse inoculations, and five QTLs for late leafspot were identified using detached leaf assays. U sing cultivated species populations, 28 QTLs were identified for LLS resistance; all but one w ere minor QTLs; the major QTL was donated by an interspecific introgression line parent. Rust often occurs alongside leafspots, and rust resistance was characterized as one major QTL, plus several smaller QTLs. Marker-assisted backcrossing o f this major QTL has been performed into different populations. QTLs for resistance to other biotic stresses have been identified, namely to groundnut rosette virus, Sclerotinia blight, afiatoxin contamination, aphids, and tomato spotted w ilt virus. Marker-assisted breeding is still in early stages, and develop ment o f more rapid and inexpensive markers from transcriptome and genom e sequencing is expected to accelerate progress.

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Identification of genes whose transcripts accumulate rapidly in tomato after root-knot nematode infection

Cited by 46 publications

References 29 publications

Biochemical and genetic studies of two Heterodera avenae resistance genes transferred from Aegilops ventricosa to wheat

Biochemical and genetic studies of two Heterodera avenae resistance genes transferred from Aegilops ventricosa to wheat

Comprehensive Transcriptome Profiling in Tomato Reveals a Role for Glycosyltransferase in Mi-Mediated Nematode Resistance

Marker‐Assisted Selection for Biotic Stress Resistance in Peanut

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