Identification of genes up-regulated during somatic embryogenesis of cucumber

Wiśniewska, Anita; Grabowska, Agnieszka; Pietraszewska-Bogiel, Anna; Tagashira, Norikazu; Zuzga, Sabina; Wóycicki, Rafał; Przybecki, Zbigniew; Malepszy, S.; Filipecki, Marcin

doi:10.1016/j.plaphy.2011.09.017

Cited by 17 publications

(17 citation statements)

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“…Most of the available data on SE-involved transcriptomes was provided by global analytical approaches and among these microarray analysis has been intensively applied to investigate the embryogenic cultures of different plants including oil palm (Low et al 2008), Medicago truncatula (Mantiri et al 2008a), potato (Sharma et al 2008), rice (Chakrabarty et al 2010) and cucumber (Wiśniewska et al 2012). Besides microarrays, EST sequencing in wheat (Singla et al 2007) and RNA-seq in Arabidopsis (Wickramasuriya and Dunwell 2015) and cotton (Yang et al 2012) have been applied in order to reveal SE-related transcriptomes.…”

Section: Global Characteristics Of the Se-associated Tf Transcriptomesmentioning

confidence: 99%

Transcription Factors in the Regulation of Somatic Embryogenesis

Nowak

Gaj

2016

Somatic Embryogenesis: Fundamental Aspects and Applications

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Somatic embryogenesis (SE), the process through which already differentiated cells reverse their developmental programme and become embryogenic, requires drastic changes in the transcriptome of the explant cells. Among the various factors that underlie this developmental switch, genes encoding transcription factors (TFs), which constitute the sequence-specific DNA-binding proteins, are widely accepted as playing a central function in the gene expression regulation. In recent years, intensive analysis of the global transcriptomes of plant cells that are undergoing embryogenic transition and the use of Arabidopsis (a model in plant genomics) in studies on the genetic control of SE have substantially contributed to the identification of SE regulators. A survey of SE-associated transcriptomes illustrated the combinational effects of stress and hormone signalling that are related to the in vitro environment that is imposed during a culture. Accordingly, among the TFs that are considered to be essential in SE induction, those that are involved in stress and hormone plant responses and especially flower development were found to be most frequent. This chapter provides a comprehensive review of the current knowledge about the TFs that are involved in the induction of SE in plant explants that are cultured in vitro. In addition to a general characterisation of the TF transcriptomes that are associated with SE induction in different plants, the individual TF genes with documented functions in the regulation of SE are presented with a special reference to their possible targets and the TF-controlled molecular mechanisms that underlie SE induction.

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Section: Global Characteristics Of the Se-associated Tf Transcriptomesmentioning

confidence: 99%

Transcription Factors in the Regulation of Somatic Embryogenesis

Nowak

Gaj

2016

Somatic Embryogenesis: Fundamental Aspects and Applications

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“…Work done so far in this area suggested that reprogramming of cellular fate is due to pattern change in the expression of candidate genic-factors in response to diverse stress conditions. Such important factors may in turn trigger the cellular modulation for embryo formation (Feher et al 2003 ;Chugh and Khurana 2002 ;Ge et al 2012 ;Namasivayam 2007 ;Rose and Nolan 2006 ;Schlögl et al 2012 ;Wiśniewskaa et al 2012 ;Wójcikowska et al 2013 ;Xu et al 2013 ;Zheng et al 2013 ). It is evident that phytohormones' level is critical for the induction of SE under stress conditions, which subsequently may infl uence genetic and cellular trans -factors essential for cell-restructuring and re-programming.…”

Section: Stress-mediated Up-regulation Of Phytohormone In Sementioning

confidence: 99%

Genes and Trans-Factors Underlying Embryogenic Transition in Plant Soma-Cells

Pandey

Chaudhary

2015

Advances in the Understanding of Biological Sciences Using Next Generation Sequencing (NGS) Approaches

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Reproduction is a unique feature of all living beings in order to maintain the species existence. In plant kingdom, majorly pollination, fertilization, embryo development and its germination are the natural practices to persist in the species management. Many plants choose alternate mechanisms for their genetic succession either parallel or independently. For example, in the genus Bryophyllum plantlets develop on the fringes of leaves whereas fragments of the plant bodies of liverworts and mosses regenerate through embryo formation. In many Citrus species, nucellar cells produce embryos, perhaps for overcoming the fertilization barrier and higher growth competency. Theoretically it is understood that all plant cells are totipotent and can give rise complete plantlet. If so, why do different taxa, cultivars and type of explants (tissues) exhibit signifi cant variation in the regeneration potential? It is evident that different plant species respond in vitro with a wide spectrum of regeneration methods including organogenesis and somatic embryogenesis. The latter has drawn more attention in recent past as limited success had been achieved across plant species. Somatic EmbryogenesisSomatic embryogenesis (SE) is one of the most diverged modes of regeneration in plants. Somatic embryogenesis was fi rst reported in carrot cell suspension cultures (Steward et al. 1958 ). Somatic embryogenesis is the process of transition of a

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“…A DNA probe for cDNA library screening was prepared from the 573-bp cDNA fragment identified by SSH (Wiśniewska et al 2012). An obtained CsSCR cDNA was then used as the probe for screening a cucumber genomic (EMBL-4) DNA library to identify a clone containing the 5 0 untranslated region (missing from the cDNA), an intron and the promoter sequence of the CsSCR gene.…”

Section: Cloning Of Cdna and Genomic Csscr Sequencesmentioning

confidence: 99%

“…In our laboratory, we have used cucumber somatic embryogenesis (Wróblewski et al 1995;Burza et al 2006) as a model to study differential gene expression during the early stages of cucumber development. To obtain cDNA fragments representing genes that take part in this process, the techniques of differential display (DD) and suppression subtractive hybridization (SSH) have been used (Linkiewicz et al 2004;Wiśniewska et al 2012). So far, only a few genes engaged in cucumber somatic embryogenesis have been described: CUS1, CsSEF1, Cs-XTH1 and Cs-XTH3 (Filipecki et al 1997;Linkiewicz et al 2004;Malinowski et al 2004;Grabowska et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…So far, only a few genes engaged in cucumber somatic embryogenesis have been described: CUS1, CsSEF1, Cs-XTH1 and Cs-XTH3 (Filipecki et al 1997;Linkiewicz et al 2004;Malinowski et al 2004;Grabowska et al 2009). Among our panel of cucumber cDNAs showing differential expression during SE induction, a homologue of the SCARECROW gene was identified and named Cucumis sativus SCARECROW (CsSCR) (Wiśniewska et al 2012). The SCARECROW gene from A. thaliana (AtSCR1) belongs to the GRAS family of proteins and functions as a transcription factor (Di Laurenzio et al 1996).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of SCARECROW (CsSCR) gene expressed during somatic embryo development and in root of cucumber (Cucumis sativus L.)

et al. 2012

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Somatic embryogenesis (SE) in plants can be used as a model for studying genes engaged in the embryogenic transition of somatic cells. The CsSCARECROW (CsSCR) gene was previously identified among a panel of genes upregulated after the induction of SE in cucumber (Cucumis sativus). The putative CsSCR protein contains conserved GRAS family domains and is extremely similar to AtSCR from Arabidopsis thaliana. SCR proteins are transcription factors involved in root radial patterning and are required for maintenance of the quiescent centre and differentiation of the endodermis. In comparison with other GRAS proteins from cucumber, phylogenetic analyses showed that CsSCR belongs to the SCR cluster. Increased CsSCR transcript accumulation was detected in somatic embryos and roots. Southern blot analysis and screening of the draft version of the cucumber genome confirmed the lack of close homologues in this species. CsSCR transcripts were localized by in situ hybridization in undifferentiated cells in the globular and heart stages of somatic embryogenesis, and in the endodermis of torpedo and cotyledonary stage somatic embryos, and developing primary and lateral roots. This localization was supported by the pattern of reporter gene activity driven by the CsSCR promoter in transgenic cucumber organs. These results suggest that CsSCR is likely to act in tissue radial organization during somatic embryogenesis and root development.Keywords Cucumber Á GRAS protein family Á Root anatomy Á Scarecrow Á SCR Á Somatic embryogenesis Abbreviations ECS Embryogenic cell suspension LRP Lateral root primordia SE Somatic embryogenesis Communicated by S. Werbrouck. Electronic supplementary materialThe online version of this article

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Identification of genes up-regulated during somatic embryogenesis of cucumber

Cited by 17 publications

References 54 publications

Transcription Factors in the Regulation of Somatic Embryogenesis

Transcription Factors in the Regulation of Somatic Embryogenesis

Genes and Trans-Factors Underlying Embryogenic Transition in Plant Soma-Cells

Molecular characterization of SCARECROW (CsSCR) gene expressed during somatic embryo development and in root of cucumber (Cucumis sativus L.)

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