Identification of genes required for decolorization of crystal violet in Citrobacter sp. MY-5

Kim, Ji Youn; Lee, Young Mi; Jang, Mi; Kang, Dong Woo; Kim, Seok Jo; Kim, Cheorl Ho; Lee, Young Choon

doi:10.2323/jgam.51.191

Cited by 4 publications

(4 citation statements)

References 33 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bacterial species identified through 16S rRNA nucleotide sequence and BLAST are summarized in Table 1 . Among these bacteria, the bacterium named kw was identified as Stenotrophomonas maltophilia , and this bacterium was already known to degrade Crystal Violet ( Kim et al, 2002 ).…”

Section: Resultsmentioning

confidence: 99%

Biodegradation of crystal violet by newly isolated bacteria

Kwak,

Park,

Sim

et al. 2024

PeerJ

View full text Add to dashboard Cite

Confronting the environmental threat posed by textile dyes, this study highlights bioremediation as a pivotal solution to mitigate the impacts of Crystal Violet, a widely-utilized triphenylmethane dye known for its mutagenic and mitotic toxicity. We isolated and identified several bacterial strains capable of degrading Crystal Violet under various environmental conditions. Newly identified strains, including Mycolicibacterium nivoides, Chryseobacterium sp., Agrobacterium rhizogenes, Pseudomonas crudilactis, and Pseudomonas koreensis demonstrated significant decolorization activity of Crystal Violet, complementing the already known capabilities of Stenotrophomonas maltophilia. Initial experiments using crude extracts confirmed their degradation potential, followed by detailed studies that investigated the impact of different pH levels and temperatures on some strains’ degradation efficiency. Depending on the bacteria, the degree of activity change according to pH and temperature was different. At 37 °C, Chryseobacterium sp. and Stenotrophomonas maltophilia exhibited higher degradation activity compared to 25 °C, while Pseudomonas crudilactis and Mycolicibacterium nivoides did not exhibit a statistically significant difference between the two temperatures. Mycolicibacterium nivoides performed optimally at pH 8, while Pseudomonas crudilactis showed high activity at pH 5. Stenotrophomonas maltophilia’s activity remained consistent across the pH range. These findings not only underscore the effectiveness of these bacteria as agents for Crystal Violet degradation but also pave the way for their application in large-scale bioremediation processes for the treatment of textile effluents, marking them as vital to environmental sustainability efforts.

show abstract

Section: Resultsmentioning

confidence: 99%

Biodegradation of crystal violet by newly isolated bacteria

Kwak,

Park,

Sim

et al. 2024

PeerJ

View full text Add to dashboard Cite

show abstract

“…Crystal violet (C V) belongs totriphenylmethane dyes which are used in the clothing industry (Kim et al, 2005). It is used in the treatment of pinworms and added to feed to prevent fungal growth (Azmi et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Genetical Studies on Bioremediation of Some Chemical Pollutants - Contaminated Water Byspirulina (Arthrospirafusiformis)

Belal

Aboulila

Metwaly

et al. 2017

Journal of Agricultural Chemistry and Biotechnology

View full text Add to dashboard Cite

Bioremediation of crystal violet and ammonia was investigated by Spirulina (Arthrospirafusiformis) in aquatic system.As well as, studying effect of crystal violet against genetic structure of Spirulina using biochemical and molecular genetic techniqes was the other aim of this study. Spirulina was capable of using crystal violet and ammonia as sole sources of carbon and nitrogen, respectively.Crystal violet and ammonia were completely remediated by Spirulinain liquid medium at pH 9 and 35 °C after 25 incubation days comparing with uninoculated medium (control). No toxicity of crystal violet was appeared after 25 days of incubation with A. fusiformisandBacillus subtilisE5 as a microbial bioassay test. The dissipation of crystal violet and ammonia was coinciding with increasing A. fusiformis biomass. Estraseenzyme activity and RAPD analysis wereappliedin order to study thegenotoxic effect of crystal violet on genome structure of Spirulina. Enzyme activity of estrase was markedly differed in crystal violet treated algae in comparison with control group, indicating theinhibition of enzyme activity as bands number andhigh polymorphism percentage(87.5%) between control and treatment. Seven RAPD primers generated 44 DNA fragments, 37of them were polymorphic (84.09%).The results indicated that crystal violet induced genotoxic effect on the genomic structure of Spirulina.So, it is worth to conclude thatA. fusiformis could be applied to remediate crystal violet and ammonia contaminated water.

show abstract

“…Crystal violet falls in the class of triphenylmethane dyes which are commonly used in the clothing industry to dye wool, silk and cotton (Kim et al, 2005). It is well known as a biological stain, yet also possesses medicinal properties.…”

Section: Introductionmentioning

confidence: 99%

BIOREMEDIATION OF CHEMICAL POLLUTANTS- CONTAMINATED WATER A-BIODECOLORIZATION OF CRYSTAL VIOLET CONTAMINATED WATER BY Pseudomonas geniculata

Hauka

Belal

Selim

et al. 2014

Journal of Agricultural Chemistry and Biotechnology

View full text Add to dashboard Cite

Biodcolorization of crystal violet (C V) was investigated in aquatic system. C V decolorizing bacterium (designated isolate AT 17) was isolated from effluent sample using enrichment technique by clear zone formation. Based on morphological, physiological and 16S rDNA, this bacterium was identified as Pseudomonas geniculata. It was capable of using C V as a sole source of carbon. Nutrient agar medium inhibited C V decolorization but mineral salt medium (MSM) increased C V decolorization. C V decolorizing by P. geniculata was found to be optimum at pH 7 and 35°C. Additional carbon sources (i.e., glucose) and nitrogen sources (i.e., peptone, yeast extract and beef extract) inhibited completely C V decolorization. On the ather hand, ammonium chloride and ammonium sulphate increased the C V decolorization. Ammonium chloride was the best inorganic nitrogen source in C V decolorization. Incubation period for 7 days was the optimum for C V decolorization. P. geniculata was able to decolorize 99% of C V completely in liquid medium at pH 7 and 35 °C after 7 days comparing with uninoculated medium(control). There was no toxicity of detected C V after 7 days of incubation with P. geniculata on Bacillus subtilis as microbial bioassay test. The dissipation of C V was coinciding with increasing P. geniculata biomass in C V contaminated water. This study has shown that P.geniculata C V could be applied to remediate Chemical pollutantscontaminated water.

show abstract

Identification of genes required for decolorization of crystal violet in Citrobacter sp. MY-5

Cited by 4 publications

References 33 publications

Biodegradation of crystal violet by newly isolated bacteria

Biodegradation of crystal violet by newly isolated bacteria

Genetical Studies on Bioremediation of Some Chemical Pollutants - Contaminated Water Byspirulina (Arthrospirafusiformis)

BIOREMEDIATION OF CHEMICAL POLLUTANTS- CONTAMINATED WATER A-BIODECOLORIZATION OF CRYSTAL VIOLET CONTAMINATED WATER BY Pseudomonas geniculata

Contact Info

Product

Resources

About