Identification of Genes Related to Clinicopathological Characteristics and Prognosis of Patients with Colorectal Cancer

Gao, Xueren; Yang, Jiaojiao

doi:10.1089/dna.2019.5088

Cited by 22 publications

(21 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, our recent study identified the top 10 upregulated genes in CRC tissues compared with normal colorectal tissues (GSE21815) (our unpublished data from [10]). Although some of these top 10 upregulated CRC-associated genes are involved in CRC progression [12], the expression levels, prognostic relevance, and molecular mechanism of these genes in relation to CRC remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Expression Profile and Prognostic Value of Wnt Signaling Pathway Molecules in Colorectal Cancer

Wang

Tang

et al. 2021

Biomedicines

View full text Add to dashboard Cite

Colorectal cancer (CRC) is a heterogeneous disease with changes in the genetic and epigenetic levels of various genes. The molecular assessment of CRC is gaining increasing attention, and furthermore, there is an increase in biomarker use for disease prognostication. Therefore, the identification of different gene biomarkers through messenger RNA (mRNA) abundance levels may be useful for capturing the complex effects of CRC. In this study, we demonstrate that the high mRNA levels of 10 upregulated genes (DPEP1, KRT80, FABP6, NKD2, FOXQ1, CEMIP, ETV4, TESC, FUT1, and GAS2) are observed in CRC cell lines and public CRC datasets. Moreover, we find that a high mRNA expression of DPEP1, NKD2, CEMIP, ETV4, TESC, or FUT1 is significantly correlated with a worse prognosis in CRC patients. Further investigation reveals that CTNNB1 is the key factor in the interaction of the canonical Wnt signaling pathway with 10 upregulated CRC-associated genes. In particular, we identify NKD2, FOXQ1, and CEMIP as three CTNNB1-regulated genes. Moreover, individual inhibition of the expression of three CTNNB1-regulated genes can cause the growth inhibition of CRC cells. This study reveals efficient biomarkers for the prognosis of CRC and provides a new molecular interaction network for CRC.

show abstract

Section: Introductionmentioning

confidence: 99%

Expression Profile and Prognostic Value of Wnt Signaling Pathway Molecules in Colorectal Cancer

Wang

Tang

et al. 2021

Biomedicines

View full text Add to dashboard Cite

show abstract

“…Moreover, KRT23 was investigated to be a host factor induced by HCV in liver 18 .KimD et al 19 detected that KRT23 was one of PPARA targeted, MYC-ampli ed oncogene in HCC.Taken together, all research above highlighted that KRT23 had the potential to be a diagnostic biomarker for liver cancer 19 . Our research is the rst to systematically investigate the effect of KRT23 in HCC development and discover thatKRT23 was overexpressed in HCC tissues and cell lines.…”

Section: Discussionmentioning

confidence: 99%

KRT23 Acts as an Oncogene in Hepatocellular Carcinoma by Regulating P21 via PI3K/AKT/GSK3β Pathway

Guo¹,

Ma²,

Wang³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background:Hepatocellular carcinoma (HCC) is a leading cancer worldwide for which diagnosis, treatment and progression are largely unknown. Keratin23 is a potential biomarker forHCC development; however, regulatory mechanisms underlying its expression remain unclear. Inthis research we explored the expression and effect of KRT23 underlying HCC development. Materials and methods:GEPIA was applied to analyze the expression of KRT23 in HCC samples and Kaplan-Merier survival analysis for patients’ prognosis. Next, IHC was further conducted for confirming its expression in HCC tissues. Meanwhile qRT-PCR and western blot analysis were applied to examine the expression of KRT23 on both mRNA and protein level in HCC cell lines compared with immortal hepatocyte LO2. Cell experiments including MTT assay, apoptosis analysis, cell cycle assay and clone formation assay were conducted for cell proliferation while transwell assay and scratch test for metastasis in vitro. Moreover, xenograft tumors in nude mice were further conducted for verification in vivo. As for mechanism in depth, immunofluorescence and western blot were operated to explore the effect of KRT23 on EMT and PI3K/AKT/GSK3βsignaling pathway. Furthermore, Co-immunoprecipitation was applied for interaction between KRT23 and P21. Functional rescue experiments were conducted to further testify their mutual effect.Results:For this research, we discovered the high expression of KRT23 in HCC samples and cell lines. Functionally, KRT23 knockdown reduced cell proliferation and metastasis in vitro and vivo. Furthermore, KRT23 participated in EMT progression and interacted with P21 to mediate PI3K/AKT/GSK3βpathway in HCC development.Conclusion:To summarize, KRT23 accelerated HCC proliferation and metastasis by regulating P21 via PI3K/AKT/GSK3βpathway.

show abstract

“…Detection of CLCA4 and MS4A12 expression might also help to identify risk factors with poor prognosis in patients with PCRC [ 58 ]. Therefore, assessing the level of CLCA4 and MS4A12 could help to accurately predict the prognosis, recurrence and the potential for secondary surgery, so that each patient can be treated individually and the therapeutic effect could be optimized [ 59 ]. Currently, the European and American countries have translated the experimental results of pharmacogenomics into clinical applications [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

CLCA4 and MS4A12 as the significant gene biomarkers of primary colorectal cancer

et al. 2020

View full text Add to dashboard Cite

Background: Primary colorectal cancer (PCRC) is a common digestive tract cancer in the elderly. However, the treatment effect of PCRC is still limited, and the long-term survival rate is low. Therefore, further exploring the pathogenesis of PCRC, and searching for specific molecular targets for diagnosis are development trends of precise medical treatment, which have important clinical significance. Methods: The public data was downloaded from Gene Expression Omnibus (GEO) database. Verification for repeatability of intra-group data was performed by Pearson’s correlation test and principal component analysis. Differentially expressed genes (DEGs) between normal and PCRC were identified, and the Protein-protein interaction (PPI) network was constructed. Significant module and hub genes were found in the PPI network. A total of 192 PCRC patients were recruited between 2010 and 2019 from the Fourth Hospital of Hebei Medical University. RT-PCR was used to measure the relative expression of CLCA4 and MS4A12. Furthermore, the study explored the effect of expression of CLCA4 and MS4A12 for overall survival. Results: A total of 53 DEGs were identified between PCRC and normal colorectal tissues. Ten hub genes concerned to PCRC were screened, namely CLCA4, GUCA2A, GCG, SST, MS4A12, PLP1, CHGA, PYY, VIP, and GUCA2B. The PCRC patients with low expression of CLCA4 and MS4A12 has a worse overall survival than high expression of CLCA4 and MS4A12 (P<0.05). Conclusion: The research of DEGs in PCRC (53 DEGs, 10 hub genes, especially CLCA4 and MS4A12) and related signaling pathways is conducive to the differential analysis of the molecular mechanism of PCRC.

show abstract

Identification of Genes Related to Clinicopathological Characteristics and Prognosis of Patients with Colorectal Cancer

Cited by 22 publications

References 39 publications

Expression Profile and Prognostic Value of Wnt Signaling Pathway Molecules in Colorectal Cancer

Expression Profile and Prognostic Value of Wnt Signaling Pathway Molecules in Colorectal Cancer

KRT23 Acts as an Oncogene in Hepatocellular Carcinoma by Regulating P21 via PI3K/AKT/GSK3β Pathway

CLCA4 and MS4A12 as the significant gene biomarkers of primary colorectal cancer

Contact Info

Product

Resources

About