Identification of genes involved in xylose metabolism of Meyerozyma guilliermondii and their genetic engineering for increased xylitol production

Atzmüller, Denise; Ullmann, Nadine

doi:10.1186/s13568-020-01012-8

Cited by 24 publications

(8 citation statements)

References 53 publications

(70 reference statements)

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“…The xylitol production capability of M. guilliermondii isolated from honeybee samples was better than the previous report of the M. guilliermondii B12 strain isolated from sugarcane bagasse with the maximum xylitol yield of approximately 0.32 g/g [ 68 ]. Recently, the overexpression of XYL1 and knockout of XDH1 genes encoding in M. guilliermondii ATCC6260 promotes xylitol production with specific productivity of 0.27 g/g xylose [ 38 ]. However, the M. guilliermondii MX strain did not show accumulation of extracellular glycerol when exposed to high-salt stress as previously described [ 69 ].…”

Section: Resultsmentioning

confidence: 99%

“…A large amount of evidence indicates that yeasts could be used as a suitable platform for chemical production of valuable metabolites. Some yeast species can produce xylitol as main product, such as Scheffersomyces [ 35 , 36 ], Kluyveromyces [ 37 ] and Meyerozyma [ 38 ]. Recently, Meyerozyma guilliermondii B12 strain has been the best isolated strain from sugarcane bagasse due to its capability to produce xylitol with 0.30 g xylitol/g xylose consumed [ 39 ].…”

Section: Introductionmentioning

confidence: 99%

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Selection of Potential Yeast Probiotics and a Cell Factory for Xylitol or Acid Production from Honeybee Samples

et al. 2021

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Excessive use of antibiotics has detrimental consequences, including antibiotic resistance and gut microbiome destruction. Probiotic-rich diets help to restore good microbes, keeping the body healthy and preventing the onset of chronic diseases. Honey contains not only prebiotic oligosaccharides but, like yogurt and fermented foods, is an innovative natural source for probiotic discovery. Here, a collection of three honeybee samples was screened for yeast strains, aiming to characterize their potential in vitro probiotic properties and the ability to produce valuable metabolites. Ninety-four isolates out of one-hundred and four were able to grow at temperatures of 30 °C and 37 °C, while twelve isolates could grow at 42 °C. Fifty-eight and four isolates displayed the ability to grow under stimulated gastrointestinal condition, at pH 2.0–2.5, 0.3% (w/v) bile salt, and 37 °C. Twenty-four isolates showed high autoaggregation of 80–100% and could utilize various sugars, including galactose and xylose. The cell count of these isolates (7–9 log cfu/mL) was recorded and stable during 6 months of storage. Genomic characterization based on the internal transcribed spacer region (ITS) also identified four isolates of Saccharomyces cerevisiae displayed good ability to produce antimicrobial acids. These results provided the basis for selecting four natural yeast isolates as starter cultures for potential probiotic application in functional foods and animal feed. Additionally, these S. cerevisiae isolates also produced high levels of acids from fermented sugarcane molasses, an abundant agricultural waste product from the sugar industry. Furthermore, one of ten identified isolates of Meyerozyma guilliermondiii displayed an excellent ability to produce a pentose sugar xylitol at a yield of 0.490 g/g of consumed xylose. Potentially, yeast isolates of honeybee samples may offer various biotechnological advantages as probiotics or metabolite producers of multiproduct-based lignocellulosic biorefinery.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Selection of Potential Yeast Probiotics and a Cell Factory for Xylitol or Acid Production from Honeybee Samples

et al. 2021

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“…By contrast, five genes of the pentose and glucuronate interconversion and two of pentose phosphate pathways were significantly suppressed under glucose compared to growth under xylose. As expected, significant overrepresentation of genes encoding d -xylulose reductase/ l -iditol 2-dehydrogenase (EC:1.1.1.9/EC:1.1.1.14), EHS25_007055 (log2FC = −5.1 and FDR = 2.0 × 10 −51 ) and EHS25_005852 (log2FC = −3.8 and FDR = 4.0 × 10 −31 ), which catalyses the oxidation of xylitol to d -xylulose [ 69 ] was observed in xylose relative to glucose ( Figure 5 a). Xylulokinase (EC:2.7.1.17), EHS25_008536 (log2FC = −4.7 and FDR = 5.0 × 10 −37 ), which catalyses xylulose to xylulose-5-phosphate phosphorylation was also overrepresented under xylose.…”

Section: Resultsmentioning

confidence: 99%

Global Transcriptome Profile of the Oleaginous Yeast Saitozyma podzolica DSM 27192 Cultivated in Glucose and Xylose

Aliyu

Gorte

Neumann

et al. 2021

JoF

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Unlike conventional yeasts, several oleaginous yeasts, including Saitozyma podzolica DSM 27192, possess the innate ability to grow and produce biochemicals from plant-derived lignocellulosic components such as hexose and pentose sugars. To elucidate the genetic basis of S. podzolica growth and lipid production on glucose and xylose, we performed comparative temporal transcriptome analysis using RNA-seq method. Approximately 3.4 and 22.2% of the 10,670 expressed genes were differentially (FDR < 0.05, and log2FC > 1.5) expressed under batch and fed batch modes, respectively. Our analysis revealed that a higher number of sugar transporter genes were significantly overrepresented in xylose relative to glucose-grown cultures. Given the low homology between proteins encoded by most of these genes and those of the well-characterised transporters, it is plausible to conclude that S. podzolica possesses a cache of putatively novel sugar transporters. The analysis also suggests that S. podzolica potentially channels carbon flux from xylose via both the non-oxidative pentose phosphate and potentially via the first steps of the Weimberg pathways to yield xylonic acid. However, only the ATP citrate lyase (ACL) gene showed significant upregulation among the essential oleaginous pathway genes under nitrogen limitation in xylose compared to glucose cultivation. Combined, these findings pave the way toward the design of strategies or the engineering of efficient biomass hydrolysate utilization in S. podzolica for the production of various biochemicals.

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“…Our previous study in protoplast fusion between S. cerevisiae and P. stipitis obtained fusant, fusant which appear decrease in bioethanol production compare with a parent were selected for current study on xylitol production. [4] reported that Meyerozyma guilliermondii, a non-conventional yeast, is being considered as a biotechnological candidate for xylitol processing. This is in agreement with the aforementioned [33] about the improvement of xylitol production from sugarcane bagasse by using phylogenetically identified as Pichia fermentans isolated from a chemical mutagenesis using ethyl methanesulfonate (EMS).…”

Section: Xylitol Production From Batch Fermentation Of Hydrolase Hemicellulose Using Meyerozyma Guilliermondii Strain F22mentioning

confidence: 99%

Fermentation Processes By Meyerozyma Guilliermondii And Saccharomyces Cerevisiae For Co-production Of Xylitol And Bioethanol Co-Production

Shalsh¹,

Nagimm²,

Alrheem³

et al. 2021

Al-Qadisiyah Journal of Pure Science

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Recent years have seen an increase in the use of lignocellulosic materials in the development of bioproduct, biorefinery technologies have focused on process integration for the production of different valuable coproducts in order to reduce the overall processing cost. In this study, agricultural wastes from rice straw were used for the co-production of bioethanol and xylitol. Where bioethanol is produced from the cellulosic fraction and xylitol from the hemicellulose fraction after elimination of lignin using chemical pretreatments. The chemical treatment was carried out with diluted acid 2.5% at a 100 °C for 30 minutes , and then exposed the cellulosic fraction of the solid phase resulting from the chemical process to the enzymatic action of the fungus Trichoderma harzianum for releas sugars and fermented at a later stage using Saccharomyces cerecvisae for bioethanol production in a simultaneous saccharification and fermentation process, The liquid phase hemicellulose fraction was exposed to action of Meyerozyma guilliermondii strain F22 (Pichia guilliermondii) for xylitol production. Resulting was accomplished yielding maximum concentrations and product yield were 32.6 g/L 0.39g/g and 20.1 g/L, 0.44g/g for bioethanol and xylitol respectively of the total glucose and xylose available in rice straw, the co-production of xylitol with ethanol in an integrated biorefinery would create economic benefits making the overall lignocellulose-based process more cost effective

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Identification of genes involved in xylose metabolism of Meyerozyma guilliermondii and their genetic engineering for increased xylitol production

Cited by 24 publications

References 53 publications

Selection of Potential Yeast Probiotics and a Cell Factory for Xylitol or Acid Production from Honeybee Samples

Selection of Potential Yeast Probiotics and a Cell Factory for Xylitol or Acid Production from Honeybee Samples

Global Transcriptome Profile of the Oleaginous Yeast Saitozyma podzolica DSM 27192 Cultivated in Glucose and Xylose

Fermentation Processes By Meyerozyma Guilliermondii And Saccharomyces Cerevisiae For Co-production Of Xylitol And Bioethanol Co-Production

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