Identification of genes expressed during Arabidopsis thaliana embryogenesis using enhancer trap and gene trap Ds-transposons

Vroemen, C.W.; Aarts, Nicole; Rieden, Paul M.J In der; Kammen, A. van; Vries, S.C. de

doi:10.1007/978-3-642-72117-5_19

Cited by 5 publications

(17 citation statements)

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“…For induction of ISR or isolation of RNA or DNA from leaves, seeds of wild-type Arabidopsis thaliana accessions Landsberg erecta (Ler-0), enhancer trap line WET121, starter lines Ac2 and DsE3 (Vroemen et al, 1998), and AtTLP1 knockout mutant line SGT5141 (Parinov et al, 1999) were sown in quartz sand. WET121, SGT5141, Ac2 and DsE3 lines were in the Ler-0 background.…”

Section: Plant Growth Conditionsmentioning

confidence: 99%

“…Vroemen (Wageningen University, The Netherlands). Construction of the gene trap and enhancer trap lines (Vroemen et al, 1998) was based on transposable elements of the Ac/Ds system carrying the GUS reporter gene (Sundaresan et al, 1995). For the selection procedure, seeds were surface sterilized with 2% (w/v) sodium hypochlorite in 50% (v/v) ethanol and sown on 0.6% (w/v) agar plates containing 0.46% (w/v) Murashige and Skoog (MS) salts (Duchefa BV, Haarlem, the Netherlands), 1.0% (w/v) sucrose and 50 mg l )1 kanamycin to select for plants with the gene/enhancer trap construct among the heterozygous lines.…”

Section: Gene Trap and Enhancer Trap Screeningmentioning

confidence: 99%

“…Isolation of genomic DNA and Southern blot analysis were performed as described previously (Vroemen et al, 1998). To amplify genomic sequences flanking the Ds insertion in WET121, TAIL-PCR was performed essentially as described (Liu et al, 1995;Vroemen et al, 1998).…”

Section: Analysis Of Flanking Sequences Of the Ds Insertion In Wet121mentioning

confidence: 99%

“…To amplify genomic sequences flanking the Ds insertion in WET121, TAIL-PCR was performed essentially as described (Liu et al, 1995;Vroemen et al, 1998). In brief, two arbitrary degenerate (AD) primers, and three specific nested Ds primers were used to amplify the Ds flanking sequences in a primary, a secondary and a tertiary round of TAIL-PCR.…”

Section: Analysis Of Flanking Sequences Of the Ds Insertion In Wet121mentioning

confidence: 99%

“…In a complementary approach to identify novel defense-related genes that are potentially involved in rhizobacteria-mediated ISR, we screened a collection of gene trap and enhancer trap lines with transposable elements of the Ac/Ds system carrying the b-glucuronidase (GUS) reporter gene (Sundaresan et al, 1995;Vroemen et al, 1998), for genes that are specifically expressed in response to colonization of the roots by ISR-inducing WCS417r bacteria. Here, we identified a thaumatin-like gene (AtTLP1) that is specifically expressed in the root vascular bundle after colonization by fluorescent Pseudomonas spp., and after treatment with the ET precursor 1-aminocyclopropane-1-carboxylate (ACC).…”

Section: Introductionmentioning

confidence: 99%

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Colonization of the Arabidopsis rhizosphere by fluorescent Pseudomonas spp. activates a root-specific, ethylene-responsive PR-5 gene in the vascular bundle

et al. 2005

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Plants of which the roots are colonized by selected strains of non-pathogenic, fluorescent Pseudomonas spp. develop an enhanced defensive capacity against a broad spectrum of foliar pathogens. In Arabidopsis thaliana, this rhizobacteria-induced systemic resistance (ISR) functions independently of salicylic acid but requires responsiveness to jasmonic acid and ethylene. In contrast to pathogen-induced systemic acquired resistance (SAR), ISR is not associated with systemic changes in the expression of genes encoding pathogenesis-related (PR) proteins. To identify genes that are specifically expressed in response to colonization of the roots by ISRinducing Pseudomonas fluorescens WCS417r bacteria, we screened a collection of Arabidopsis enhancer trap and gene trap lines containing a transposable element of the Ac/Ds system and the GUS reporter gene. We identified an enhancer trap line (WET121) that specifically showed GUS activity in the root vascular bundle upon colonization of the roots by WCS417r. Fluorescent Pseudomonas spp. strains P. fluorescens WCS374r and P. putida WCS358r triggered a similar expression pattern, whereas ISR-non-inducing Escherichia coli bacteria did not. Exogenous application of the ethylene precursor 1-aminocyclopropane-1-carboxylate (ACC) mimicked the rhizobacteria-induced GUS expression pattern in the root vascular bundle, whereas methyl jasmonic acid and salicylic acid did not, indicating that the Ds element in WET121 is inserted in the vicinity of an ethylene-responsive gene. Analysis of the expression of the genes in the close vicinity of the Ds element revealed AtTLP1 as the gene responsible for the in cis activation of the GUS reporter gene in the root vascular bundle. AtTLP1 encodes a thaumatin-like protein that belongs to the PR-5 family of PR proteins, some of which possess antimicrobial properties. AtTLP1 knockout mutant plants showed normal levels of WCS417r-mediated ISR against the bacterial leaf pathogen Pseudomonas syringae pv. tomato DC3000, suggesting that expression of AtTLP1 in the roots is not required for systemic expression of ISR in the leaves. Together, these results indicate that induction of AtTLP1 is a local response of Arabidopsis roots to colonization by non-pathogenic fluorescent Pseudomonas spp. and is unlikely to play a role in systemic resistance.

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Section: Plant Growth Conditionsmentioning

confidence: 99%

Section: Gene Trap and Enhancer Trap Screeningmentioning

confidence: 99%

Section: Analysis Of Flanking Sequences Of the Ds Insertion In Wet121mentioning

confidence: 99%

Section: Analysis Of Flanking Sequences Of the Ds Insertion In Wet121mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Colonization of the Arabidopsis rhizosphere by fluorescent Pseudomonas spp. activates a root-specific, ethylene-responsive PR-5 gene in the vascular bundle

et al. 2005

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Flowering Plant Embryogenesis

Vroemen¹,

Vries²

1999

Development

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Knoester

Sluis

Pieterse

2001

European Journal of Plant Pathology

186

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Selected strains of rhizosphere bacteria reduce disease by activating a resistance mechanism in the plant named rhizobacteria-mediated induced systemic resistance (ISR). Rhizobacteria-mediated ISR resembles pathogeninduced systemic acquired resistance (SAR) in that both types of induced resistance render uninfected plant parts more resistant towards a broad spectrum of plant pathogens. Some rhizobacteria trigger the salicylic acid (SA)dependent SAR pathway by producing SA at the root surface. In other cases, rhizobacteria trigger a different signalling pathway that does not require SA. The existence of a SA-independent ISR pathway has been demonstrated in Arabidopsis thaliana. In contrast to pathogen-induced SAR, ISR induced by Pseudomonas fluorescens WCS417r is independent of SA accumulation and pathogenesis-related (PR) gene activation but, instead, requires responsiveness to the plant hormones jasmonic acid (JA) and ethylene. Mutant analyses showed that ISR follows a novel signalling pathway in which components from the JA and ethylene response are successively engaged to trigger a defensive state that, like SAR, is controlled by the regulatory factor NPR1. Interestingly, simultaneous activation of both the JA/ethylene-dependent ISR pathway and the SA-dependent SAR pathway results in an enhanced level of protection. Thus combining both types of induced resistance provides an attractive tool for the improvement of disease control. This review focuses on the current status of our research on triggering, signalling, and expression of rhizobacteria-mediated ISR in Arabidopsis.

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Identification of genes expressed during Arabidopsis thaliana embryogenesis using enhancer trap and gene trap Ds-transposons

Cited by 5 publications

References 60 publications

Colonization of the Arabidopsis rhizosphere by fluorescent Pseudomonas spp. activates a root-specific, ethylene-responsive PR-5 gene in the vascular bundle

Colonization of the Arabidopsis rhizosphere by fluorescent Pseudomonas spp. activates a root-specific, ethylene-responsive PR-5 gene in the vascular bundle

Flowering Plant Embryogenesis

Untitled

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