Identification of Genes Encoding Amino Acid Permeases by Inactivation of Selected ORFs from the <i>Synechocystis</i> Genomic Sequence

Quintero, Marı́a José; Montesinos, Marı́a Luz; Herrero, Antonia; Flores, Enrique

doi:10.1101/gr.196301

Cited by 59 publications

(74 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6). Addition of Glu did not compensate for the growth defect in the mutant, despite the fact that this cyanobacterium possesses a Glu transporter (Quintero et al, 2001). Pyruvate mitigated the inhibition of growth to some extent.…”

Section: Effects Of Addition Of Metabolites On the Dcyabrb2 Mutant Unmentioning

confidence: 96%

Deletion of the Transcriptional Regulator cyAbrB2 Deregulates Primary Carbon Metabolism in Synechocystis sp. PCC 6803

Kaniya¹,

Kizawa²,

Miyagi

et al. 2013

Plant Physiology

View full text Add to dashboard Cite

cyAbrB is a transcriptional regulator unique to and highly conserved among cyanobacterial species. A gene-disrupted mutant of cyabrB2 (sll0822) in Synechocystis sp. PCC 6803 exhibited severe growth inhibition and abnormal accumulation of glycogen granules within cells under photomixotrophic conditions. Within 6 h after the shift to photomixotrophic conditions, sodium bicarbonate-dependent oxygen evolution activity markedly declined in the DcyabrB2 mutant, but the decrease in methyl viologen-dependent electron transport activity was much smaller, indicating inhibition in carbon dioxide fixation. Decreases in the transcript levels of several genes related to sugar catabolism, carbon dioxide fixation, and nitrogen metabolism were also observed within 6 h. Metabolome analysis by capillary electrophoresis mass spectrometry revealed that several metabolites accumulated differently in the wild-type and mutant strains. For example, the amounts of pyruvate and 2-oxoglutarate (2OG) were significantly lower in the mutant than in the wild type, irrespective of trophic conditions. The growth rate of the DcyabrB2 mutant was restored to a level comparable to that under photoautotrophic conditions by addition of 2OG to the growth medium under photomixotrophic conditions. Activities of various metabolic processes, including carbon dioxide fixation, respiration, and nitrogen assimilation, seemed to be enhanced by 2OG addition. These observations suggest that cyAbrB2 is essential for the active transcription of genes related to carbon and nitrogen metabolism upon a shift to photomixotrophic conditions. Deletion of cyAbrB2 is likely to deregulate the partition of carbon between storage forms and soluble forms used for biosynthetic purposes. This disorder may cause inactivation of cellular metabolism, excess accumulation of reducing equivalents, and subsequent loss of viability under photomixotrophic conditions.

show abstract

Section: Effects Of Addition Of Metabolites On the Dcyabrb2 Mutant Unmentioning

confidence: 96%

Deletion of the Transcriptional Regulator cyAbrB2 Deregulates Primary Carbon Metabolism in Synechocystis sp. PCC 6803

Kaniya¹,

Kizawa²,

Miyagi

et al. 2013

Plant Physiology

View full text Add to dashboard Cite

show abstract

“…PCC 6803 was the only known glutamate transporter in cyanobacteria. 9) We searched for the homologous gene in A. halophytica, and based on shotgun sequencing of A. halophytica, we found that A. halophytica contains a gene (ApgltS) which has low homology to SygltS (slr1145) of Synechocystis sp. PCC 6803.…”

Section: Resultsmentioning

confidence: 99%

“…PCC 6803 is mediated by GltS and Gtr, although the involvement of yet another transporter has been suggested. 9) However, the molecular properties of GltS have not been explored in any cyanobacterium.…”

mentioning

confidence: 99%

“…PCC 6803. 9) In Anabaena sp. PCC 7120, three amino acid transporters (N-I, N-II, and Bgt) have been reported, 8) all of them ABC transporters.…”

mentioning

confidence: 99%

“…PCC 6803, four transporters (Nat, Bgt, GltS, and Gtr) have been reported. 9) The Nat system transports histidine and neutral amino acids. GltS is a monocomponent sodium-dependent glutamate transporter.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Sodium-Dependent Uptake of Glutamate by Novel ApGltS Enhanced Growth under Salt Stress of Halotolerant CyanobacteriumAphanothece halophytica

Boonburapong

Laloknam

Yamada

et al. 2012

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

Current Awareness

2002

Comp Funct Genom

View full text Add to dashboard Cite

In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on comparative and functional genomics. Each bibliography is divided into 16 sections. 1 Reviews & symposia; 2 General; 3 Large‐scale sequencing and mapping; 4 Genome evolution; 5 Comparative genomics; 6 Gene families and regulons; 7 Pharmacogenomics; 8 Large‐scale mutagenesis programmes; 9 Functional complementation; 10 Transcriptomics; 11 Proteomics; 12 Protein structural genomics; 13 Metabolomics; 14 Genomic approaches to development; 15 Technological advances; 16 Bioinformatics. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted

show abstract

Identification of Genes Encoding Amino Acid Permeases by Inactivation of Selected ORFs from the Synechocystis Genomic Sequence

Cited by 59 publications

References 19 publications

Deletion of the Transcriptional Regulator cyAbrB2 Deregulates Primary Carbon Metabolism in Synechocystis sp. PCC 6803

Deletion of the Transcriptional Regulator cyAbrB2 Deregulates Primary Carbon Metabolism in Synechocystis sp. PCC 6803

Sodium-Dependent Uptake of Glutamate by Novel ApGltS Enhanced Growth under Salt Stress of Halotolerant CyanobacteriumAphanothece halophytica

Current Awareness

Contact Info

Product

Resources

About