Identification of genes differentially expressed in mouse mammary epithelium transformed by an activated β-catenin

Renou, Jean-Pierre; Bierie, Brian; Miyoshi, Keiko; Cui, Yongzhi; Djiane, Jean; Reichenstein, Moshe; Shani, Moshe; Hennighausen, Lothar

doi:10.1038/sj.onc.1206596

Cited by 34 publications

(29 citation statements)

References 35 publications

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“…This observation indicates that the stage of involution is distinct from all other stages of mammary development, an observation that has been confirmed at the RNA level. 47 The intensity of SMA labeling in myoepithelial cells was not influenced by lactation. This result was consistent with results of studies in women that indicated that SMA expression is not up-regulated in the lactating, relative to the resting mammary gland.…”

Section: Sequential Expression Of Differentiation Markers During Mammmentioning

confidence: 92%

Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development

et al. 2006

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Abstract. Immunohistochemical analysis using paraffin-embedded specimens is the method of choice to evaluate protein expression at a cellular level while preserving tissue architecture in normal and neoplastic tissues. Current knowledge of the expression of terminal differentiation markers in the mouse mammary gland relies on the evaluation of frozen tissues by use of immunofluorescence. We assessed changes in patterns of expression of terminal differentiation markers throughout the development of the mouse mammary gland in paraffin-embedded tissues. The expression of a-smooth muscle actin (SMA) and keratins (K) 5, 8/18, and 14 was influenced by the development stage of the mammary gland. Expression of K5 and SMA was restricted to basal cells. Keratin 14 was consistently expressed by mammary basal cells, and was detected in scattered luminal cells from 13.5 days after conception through puberty. Labeling for K8/18 of luminal cells was heterogeneous at all times. Heterogeneous expression patterns in luminal cells suggest this layer has cells with a variety of biological functions. The absence of K6 expression at any stage of the development of the mammary gland was confirmed by use of reverse transcriptase-polymerase chain reaction analysis, which indicates that this intermediate filament is not a marker of the mammary gland stem cell. Finally, consistent with results of earlier studies, keratins 1, 10, 13, and 15, and filaggrin, involucrin, and loricrin were not detected at any stage of mammary gland development.

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Section: Sequential Expression Of Differentiation Markers During Mammmentioning

confidence: 92%

Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development

et al. 2006

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“…Tissues that are believed to share certain characteristics might be expected to exhibit similar signaling endpoints. For example, Bierie et al (29) make mention that a portion of those genes upregulated in prostate tissue bearing mutant ␤-catenin expression overlap with those similarly up-regulated in mammary gland (63). Although mutant ␤-catenin expression imposed a squamous transdifferentiation program on prostate and mammary glands-indeed, this phenotype itself may partly account for the shared differential gene expression-one could argue that the overlap in gene induction is related to the general degree of similarity held between the function and hormonal regulation of these tissues.…”

Section: Discussionmentioning

confidence: 99%

Identification of Aryl Hydrocarbon Receptor as a Putative Wnt/β-Catenin Pathway Target Gene in Prostate Cancer Cells

Chesire¹,

Dunn²,

Ewing³

et al. 2004

Cancer Research

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“…We previously identified Elf5 as a gene highly upregulated in lactating mammary tissue (Renou et al 2003). Like STAT5A/5B, ELF5 is a mediator of the prolactinreceptor signaling pathway (Harris et al 2006).…”

Section: Stat5a Is Necessary and Sufficient For The Generation Of Alvmentioning

confidence: 99%

Development of mammary luminal progenitor cells is controlled by the transcription factor STAT5A

Yamaji¹,

Na²,

Feuermann³

et al. 2009

Genes Dev.

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126

137

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Mammary alveologenesis is abrogated in the absence of the transcription factors STAT5A/5B, which mediate cytokine signaling. To reveal the underlying causes for this developmental block, we studied mammary stem and progenitor cells. While loss of STAT5A/5B did not affect the stem cell population and its ability to form mammary ducts, luminal progenitors were greatly reduced and unable to form alveoli during pregnancy. Temporally controlled expression of transgenic STAT5A in mammary epithelium lacking STAT5A/5B restored the luminal progenitor population and rescued alveologenesis in a reversible fashion in vivo. Thus, STAT5A is necessary and sufficient for the establishment of luminal progenitor cells.Supplemental material is available at http://www.genesdev.org.

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Identification of genes differentially expressed in mouse mammary epithelium transformed by an activated β-catenin

Cited by 34 publications

References 35 publications

Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development

Expression of Terminal Differentiation Proteins Defines Stages of Mouse Mammary Gland Development

Identification of Aryl Hydrocarbon Receptor as a Putative Wnt/β-Catenin Pathway Target Gene in Prostate Cancer Cells

Development of mammary luminal progenitor cells is controlled by the transcription factor STAT5A

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