Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera

Dong, Du-Juan; He, Hong-Juan; Chai, Lian‐Qin; Jiang, Xiaojuan; Wang, Jin-Xing; Zhao, Xiao‐Fan

doi:10.1186/1471-213x-7-73

Cited by 36 publications

(40 citation statements)

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“…It was known that EcR , USP , E74 , E75 , and HR3 are molting transcriptional factors, which express earlier during the molting cascade [28]. We have also reported that ecdy , carbA2 , NTF2 , and G-proγ are upregulated in molting larvae or metamorphic committed larvae, which might be the late genes involved in the molting process because they are effector genes [30]. The expressions of these genes rely on the existence of 20E in the HaEpi cell line, which reveals the hormonal regulation patterns on these genes.…”

Section: Discussionmentioning

confidence: 74%

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Establishment of a New Cell Line from Lepidopteran Epidermis and Hormonal Regulation on the Genes

et al. 2008

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When an insect molts, old cuticle on the outside of the integument is shed by apolysis and a new cuticle is formed under the old one. This process is completed by the epidermal cells which are controlled by 20-hydroxyecdysone (20E) and juvenile hormone. To understand the molecular mechanisms of integument remolding and hormonal regulation on the gene expression, an epidermal cell line from the 5th instar larval integument of Helicoverpa armigera was established and named HaEpi. The cell line has been cultured continuously for 82 passages beginning on June 30, 2005 until now. Cell doubling time was 64 h. The chromosomes were granular and the chromosome mode was from 70 to 76. Collagenase I was used to detach the cells from the flask bottom. Non-self pathogen AcMNPV induced the cells to apoptosis. The cell line was proved to be an epidermal cell line based on its unique gene expression pattern. It responded to 20E and the non-steroidal ecdysone agonist RH-2485. Its gene expression could be knocked down using RNA interference. Various genes in the cell line were investigated based on their response to 20E. This new cell line represents a platform for investigating the 20E signaling transduction pathway, the immune response mechanism in lepidopteran epidermis and interactions of the genes.

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Section: Discussionmentioning

confidence: 74%

“…We previously reported that Ha-cathL was expressed only in haemocytes [30]. So we used Ha-cathL to exclude the possibility of haemocyte contamination in the HaEpi cell line.…”

Section: Discussionmentioning

confidence: 99%

Establishment of a New Cell Line from Lepidopteran Epidermis and Hormonal Regulation on the Genes

et al. 2008

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“…, 2002). An expression sequence tag (EST) of carboxypeptidase A from H. armigera was detected as up‐regulated during larval molting by suppression subtractive hybridization (SSH) (Dong et al. , 2007).…”

Section: Introductionsupporting

confidence: 53%

Characterization and influences of classical insect hormones on the expression profiles of a molting carboxypeptidase A from the cotton bollworm (Helicoverpa armigera)

Sui

Liu

Chai

et al. 2009

Insect Molecular Biology

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Molting is a very important physiological behavior to arthropods. During molting, integument apolysis occurs, which is the digestion and absorption of the old endocuticle for new cuticle formation. Proteases play critical roles in this process. Molting carboxypeptidase A ( Ha-CPA ) is characterized from Helicoverpa armigera . The Ha-CPA transcript was mainly present in the integument from the 5th instar larvae. In the integument, the transcription level of the gene reached its peak at the 5th instar molting stage and the 6th instar prepupal stage, respectively. The examination of immunohistochemistry revealed that Ha-CPA could distribute into the molting fluid in the molting-and prepupal-stage larvae. The expression of Ha-CPA could be up-regulated by 20-hydroxyecdysone (20E). These facts indicate that Ha-CPA participates in the apolysis of the integument during larval molting and metamorphosis.

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“…The molting fluid fills the exuvial space between the old and the new cuticle and exhibits chitinolytic and proteolytic activities that facilitate degradation of the inner layers of the old cuticle as part of the molting process before ecdysis. Several peptidases have been identified in insect molting fluids, including trypsin-like serine peptidases, carboxypeptidases, cysteine peptidases and metallopeptidases such as aminopeptidases (Dong et al, 2007;Liu et al, 2006Liu et al, , 2009Ote et al, 2005;Rabossi et al, 2008;Samuels et al, 1993a,b;Sui et al, 2009;Wei et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Chymotrypsin-like peptidases from Tribolium castaneum: A role in molting revealed by RNA interference

Broehan

Arakane

Beeman

et al. 2010

Insect Biochemistry and Molecular Biology

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Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera

Abstract: Background: Larval molting and metamorphosis are important physiological processes in the life cycle of the holometabolous insect. We used suppression subtractive hybridization (SSH) to identify genes differentially expressed during larval molting and metamorphosis.

Cited by 36 publications

References 36 publications

Establishment of a New Cell Line from Lepidopteran Epidermis and Hormonal Regulation on the Genes

Establishment of a New Cell Line from Lepidopteran Epidermis and Hormonal Regulation on the Genes

Characterization and influences of classical insect hormones on the expression profiles of a molting carboxypeptidase A from the cotton bollworm (Helicoverpa armigera)

Chymotrypsin-like peptidases from Tribolium castaneum: A role in molting revealed by RNA interference

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