Identification of genes associated with the astrocyte-specific gene Gfap during astrocyte differentiation

Ito, Kenji; Sanosaka, Tsukasa; Igarashi, Katsuhide; Ideta-Otsuka, Maky; Aizawa, Akira; Uosaki, Yuichi; Noguchi, Azumi; Arakawa, Hirokazu; Nakashima, Kinichi; Takizawa, Takumi

doi:10.1038/srep23903

Cited by 22 publications

(22 citation statements)

References 60 publications

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“…In astrocytes and NPCs, the activity of GFAP gene in the cluster is under the regulation of different sets of TFs. TF-binding sites in GFAP gene promoter include AP-1 (Brenner, Messing, & Olsen, 2019), NFI (Tchieu et al, 2019), c-Jun (Gao et al, 2013), STAT3 (Urayama et al, 2013), peroxisome proliferator-activated receptor γ (PPAR-γ) (Rai et al, 2014) and many others (Ito et al, 2016). The products of these genes can be organized into several cascades in the transcriptional regulation of GFAP gene expression.…”

Section: Tfsmentioning

confidence: 99%

Neurochemical regulation of the expression and function of glial fibrillary acidic protein in astrocytes

Liu

et al. 2019

Glia

100

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Glial fibrillary acidic protein (GFAP), a type III intermediate filament, is a marker of mature astrocytes. The expression of GFAP gene is regulated by many transcription factors (TFs), mainly Janus kinase‐2/signal transducer and activator of transcription 3 cascade and nuclear factor κ‐light‐chain‐enhancer of activated B cell signaling. GFAP expression is also modulated by protein kinase and other signaling molecules that are elicited by neuronal activity and hormones. Abnormal expression of GFAP proteins occurs in neuroinflammation, neurodegeneration, brain edema‐eliciting diseases, traumatic brain injury, psychiatric disorders and others. GFAP, mainly in α‐isoform, is the major component of cytoskeleton and the scaffold of astrocytes, which is essential for the maintenance of astrocytic structure and shape. GFAP also has highly morphological plasticity because of its quick changes in assembling and polymerizing states in response to environmental challenges. This plasticity and its corresponding cellular morphological changes endow astrocytes the functions of physical barrier between adjacent neurons and stabilizer of extracellular environment. Moreover, GFAP colocalizes and even molecularly associates with many functional molecules. This feature allows GFAP to function as a platform for direct interactions between different molecules. Last, GFAP involves transportation and localization of other functional proteins and thus serves as a protein transport guide in astrocytes. This guiding role of GFAP involves an elastic retraction and extension cytoskeletal network that couples with GFAP reassembling, transporting, and membrane protein recycling machinery. This paper reviews our current understanding of the expression and functions of GFAP as well as their regulation.

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Section: Tfsmentioning

confidence: 99%

Neurochemical regulation of the expression and function of glial fibrillary acidic protein in astrocytes

Liu

et al. 2019

Glia

100

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“…Using a genome-wide enhanced circular chromosomal conformation capture (e4C) technique to investigate inter-chromosomal interactions, 41 ) we have recently demonstrated that several genes expressed during astrocyte differentiation were specifically associated with Gfap , raising the possibility that gene clustering may play some roles in transcriptional regulation during NSCs differentiation. 42 )…”

Section: Histone Modifications and Chromatin Accessibilitymentioning

confidence: 99%

Emerging mechanisms underlying astrogenesis in the developing mammalian brain

Takouda

Katada

Nakashima

2017

Proceedings of the Japan Academy. Ser. B: Physical and Biologic

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In the developing brain, the three major cell types, i.e., neurons, astrocytes and oligodendrocytes, are generated from common multipotent neural stem cells (NSCs). In particular, astrocytes eventually occupy a great fraction of the brain and play pivotal roles in the brain development and functions. However, NSCs cannot produce the three major cell types simultaneously from the beginning; e.g., it is known that neurogenesis precedes astrogenesis during brain development. How is this fate switching achieved? Many studies have revealed that extracellular cues and intracellular programs are involved in the transition of NSC fate specification. The former include growth factor- and cytokine-signaling, and the latter involve epigenetic machinery, including DNA methylation, histone modifications, and non-coding RNAs. Accumulating evidence has identified a complex array of epigenetic modifications that control the timing of astrocytic differentiation of NSCs. In this review, we introduce recent progress in identifying the molecular mechanisms of astrogenesis underlying the tight regulation of neuronal-astrocytic fate switching of NSCs.

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“…Astrocytes derive from NPCs that can self-renew, or differentiate into neurons, astrocytes or oligodendrocytes. These lineage commitments are typically coordinated by the activity of lineage-specific transcription factors, which in turn are known to be tightly regulated by signalling molecules, such as cytokines ( 20 ).…”

Section: Workflow Examplementioning

confidence: 99%

“…Glial fibrillary acidic protein (Gfap) is considered an astrocyte-specific gene and has been widely used in the study of astrocytogenesis ( 20 ). Ito and colleagues set out to discover genes associated with Gfap during astrocyte differentiation.…”

Section: Workflow Examplementioning

confidence: 99%

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StemMapper: a curated gene expression database for stem cell lineage analysis

Pinto

Machado

Magno

et al. 2017

Nucleic Acids Research

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Transcriptomic data have become a fundamental resource for stem cell (SC) biologists as well as for a wider research audience studying SC-related processes such as aging, embryonic development and prevalent diseases including cancer, diabetes and neurodegenerative diseases. Access and analysis of the growing amount of freely available transcriptomics datasets for SCs, however, are not trivial tasks. Here, we present StemMapper, a manually curated gene expression database and comprehensive resource for SC research, built on integrated data for different lineages of human and mouse SCs. It is based on careful selection, standardized processing and stringent quality control of relevant transcriptomics datasets to minimize artefacts, and includes currently over 960 transcriptomes covering a broad range of SC types. Each of the integrated datasets was individually inspected and manually curated. StemMapper's user-friendly interface enables fast querying, comparison, and interactive visualization of quality-controlled SC gene expression data in a comprehensive manner. A proof-of-principle analysis discovering novel putative astrocyte/neural SC lineage markers exemplifies the utility of the integrated data resource. We believe that StemMapper can open the way for new insights and advances in SC research by greatly simplifying the access and analysis of SC transcriptomic data. StemMapper is freely accessible at http://stemmapper.sysbiolab.eu.

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Identification of genes associated with the astrocyte-specific gene Gfap during astrocyte differentiation

Cited by 22 publications

References 60 publications

Neurochemical regulation of the expression and function of glial fibrillary acidic protein in astrocytes

Neurochemical regulation of the expression and function of glial fibrillary acidic protein in astrocytes

Emerging mechanisms underlying astrogenesis in the developing mammalian brain

StemMapper: a curated gene expression database for stem cell lineage analysis

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