Identification of genes associated with cold acclimation in blueberry (Vaccinium corymbosum L.) using a subtractive hybridization approach

Naik, D. M.; Dhanaraj, Anik L.; Arora, Rajeev; Rowland, Lisa J.

doi:10.1016/j.plantsci.2007.05.003

Cited by 40 publications

(25 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DYL1 is abundantly expressed in axillary buds of pea, with mRNA levels decreasing after decapitation; it is therefore considered a good marker of dormancy [81]. In blueberry buds, DRM mRNA levels were highest in a sample collected on December 8th, subsequently decreasing towards bud burst [82]. In grape buds, DRM gene expression also decreases progressively towards bud burst, this gene being more strongly expressed during dormancy [32].…”

Section: Discussionmentioning

confidence: 99%

Transcriptional profiling of bud dormancy induction and release in oak by next-generation sequencing

Ueno

Klopp²,

Leplé³

et al. 2013

BMC Genomics

View full text Add to dashboard Cite

BackgroundIn temperate regions, the time lag between vegetative bud burst and bud set determines the duration of the growing season of trees (i.e. the duration of wood biomass production). Dormancy, the period during which the plant is not growing, allows trees to avoid cold injury resulting from exposure to low temperatures. An understanding of the molecular machinery controlling the shift between these two phenological states is of key importance in the context of climatic change. The objective of this study was to identify genes upregulated during endo- and ecodormancy, the two main stages of bud dormancy. Sessile oak is a widely distributed European white oak species. A forcing test on young trees was first carried out to identify the period most likely to correspond to these two stages. Total RNA was then extracted from apical buds displaying endo- and ecodormancy. This RNA was used for the generation of cDNA libraries, and in-depth transcriptome characterization was performed with 454 FLX pyrosequencing technology.ResultsPyrosequencing produced a total of 495,915 reads. The data were cleaned, duplicated reads removed, and sequences were mapped onto the oak UniGene data. Digital gene expression analysis was performed, with both R statistics and the R-Bioconductor packages (edgeR and DESeq), on 6,471 contigs with read numbers ≥ 5 within any contigs. The number of sequences displaying significant differences in expression level (read abundance) between endo- and ecodormancy conditions ranged from 75 to 161, depending on the algorithm used. 13 genes displaying significant differences between conditions were selected for further analysis, and 11 of these genes, including those for glutathione-S-transferase (GST) and dehydrin xero2 (XERO2) were validated by quantitative PCR.ConclusionsThe identification and functional annotation of differentially expressed genes involved in the “response to abscisic acid”, “response to cold stress” and “response to oxidative stress” categories constitutes a major step towards characterization of the molecular network underlying vegetative bud dormancy, an important life history trait of long-lived organisms.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptional profiling of bud dormancy induction and release in oak by next-generation sequencing

Ueno

Klopp²,

Leplé³

et al. 2013

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…These results provide a new set of genes of potential interest Three independent biological replications were performed. All data were normalized to metallothionein expression levels [28]. Capital letters show significant differences in the susceptible cultivar (Bluegold) and lowercase letters show significant differences in the resistant cultivar (Brigitta).…”

Section: Discussionmentioning

confidence: 99%

“…The list of candidate genes and their respective primer pairs are shown in Table 1. As a housekeeping gene, the expression of metallothionein was used, as previously described [28]. All reactions were performed in triplicate.…”

Section: Real-time Rt-pcr Analysismentioning

confidence: 99%

Identification of Aluminum-Regulated Genes by cDNA-AFLP Analysis of Roots in Two Contrasting Genotypes of Highbush Blueberry (Vaccinium corymbosum L.)

Inostroza‐Blancheteau

Aquea

Reyes‐Díaz

et al. 2011

Mol Biotechnol

View full text Add to dashboard Cite

To investigate the molecular mechanisms of Al(3+)-stress in blueberry, a cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis was employed to identify Al-regulated genes in roots of contrasting genotypes of highbush blueberry (Brigitta, Al(3+)-resistant and Bluegold, Al(3+)-sensitive). Plants grown in hydroponic culture were treated with 0 and 100 μM Al(3+) and collected at different times over 48 h. Seventy transcript-derived fragments (TDFs) were identified as being Al(3+) responsive, 31 of which showed significant homology to genes with known or putative functions. Twelve TDFs were homologous to uncharacterized genes and 27 did not have significant matches. The expression pattern of several of the genes with known functions in other species was confirmed by quantitative relative real-time RT-PCR. Twelve genes of known or putative function were related to cellular metabolism, nine associated to stress responses and other transcription and transport facilitation processes. Genes involved in signal transduction, photosynthetic and energy processes were also identified, suggesting that a multitude of processes are implicated in the Al(3+)-stress response as reported previously for other species. The Al(3+)-stress response genes identified in this study could be involved in Al(3+)-resistance in woody plants.

show abstract

“…A major aim in blueberry breeding programmes is to enhance tolerance to winter freezing and spring frosts. To this end, studies have been conducted using cDNA microarrays to monitor changes in gene expression during cold acclimation of blueberry plants under field and cold room conditions Naik et al, 2007). These studies have showed that genes associated with stress tolerance are rapidly up-regulated to very high levels in stressed plants and demonstrate the potential for short duration cold treatments, and/or endogenous growth regulator treatments that up-regulated genes associated with cold tolerance, to increase bioactive levels in fruits.…”

Section: Blueberries and Raspberriesmentioning

confidence: 99%

The Influence of Pre‐ and Postharvest Environmental Stress on Fruit Bioactives

Burritt¹

2013

Bioactives in Fruit

View full text Add to dashboard Cite

Identification of genes associated with cold acclimation in blueberry (Vaccinium corymbosum L.) using a subtractive hybridization approach

Cited by 40 publications

References 52 publications

Transcriptional profiling of bud dormancy induction and release in oak by next-generation sequencing

Transcriptional profiling of bud dormancy induction and release in oak by next-generation sequencing

Identification of Aluminum-Regulated Genes by cDNA-AFLP Analysis of Roots in Two Contrasting Genotypes of Highbush Blueberry (Vaccinium corymbosum L.)

The Influence of Pre‐ and Postharvest Environmental Stress on Fruit Bioactives

Contact Info

Product

Resources

About