Identification of Fusarium solani causing basal stem and root rot of Crossandra infundibuliformis in Hainan, China

Qiu, F.; Xu, Gang; Xie, Changping; Li, X.; Zheng, F. Q.

doi:10.1007/s13313-020-00735-3

Cited by 2 publications

(2 citation statements)

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“…Observe the colony morphology and color, and measure the diameter. To observe the morphological characteristics of conidia, conidiogenous cells, and chlamydospore under the microscopic eld of view for preliminary identi cation (Qiu et al 2020). Images were processed using Adobe Photoshop CS6 software.…”

Section: Identi Cation Of Endophytic Fusarium Solani Hb1-j1mentioning

confidence: 99%

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Global regulatory factor FsveA up-regulate the production of antitumor substance in endophytic Fusarium solani

Cai¹,

Wang²,

Li³

et al. 2022

Preprint

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A number of studies have demonstrated that endophytic fungi have the potential to produce antitumor active substances with novel structures and significant activities. Our previous studies found that an endophytic Fusarium solani derived from the stem of the medicinal plant Nothapodytes pittosporoides (Oliv.) Sleum had good antitumor activity. Furthermore, overexpression of the global regulator FsveA (FsveAOE) in F. solani led to significant inhibition ratio increasement of human alveolar adenocarcino -ma cell (A549). In comparison with the wild strain (WT), the inhibition rate of FsveAOE was obviously increased ~ 14.69%, and the apoptosis ratio was significantly increased 4.86-fold. A metabolomic analysis revealed that the overexpression of FsveA resulted in a significant increasement in several antitumor secondary metabolites of fungi, including alkaloids (Geldanamycin, Acadesine, Adenosine), terpenoids (Lanosterin, Illudin M), carboxylic acid derivatives (L-Arginine, L-Methionine), phenolic (4-Allyl-2-methoxyphenol) and flavonoid metabolites (Epicatechin gallate). Additionally, the transcriptome analysis also displayed that expression pattern of 48 genes-related to the antitumor activity were significantly changed in FsveAOE, mainly involving glycosyl hydrolases, the Zn(2)-Cys(6) class, Cytochrome P450 monooxygen -ase, 3-isopropylmalate dehydratase, and polyketide synthases. Therefore, we conclude that FsveA mediate multiple secondary metabolic pathways to up-regulate the production of antitumor substance in endophytic F. solani.

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Section: Identi Cation Of Endophytic Fusarium Solani Hb1-j1mentioning

confidence: 99%

“…Ampli cation was performed using primers ITS1/ITS4 (White et al 1990), EF-1/EF-2 (Geise et al 2004), RPB1-DF2asc/RPB1-G2R (Zhang et al 2020), fRPB2-5f2/fRPB2-7cr (Qiu et al 2020). Sequencing was done on the PCR products.…”

Section: Identi Cation Of Endophytic Fusarium Solani Hb1-j1mentioning

confidence: 99%