Identification of Further Antigens on Red Cells and Lymphocytes

Abstract

Antigenic sites of Bg^b and Bg^c specificity are present on lymphocytes as well as on red cells. The Bg^b group has been correlated with the W17 (Te57) white cell group, and the Bg^c with the W28 (Da15,Ba*) antigen. Both haemagglutinating and cytotoxic antibodies can be absorbed by either red or white cells, though cross-reactivity was shown by many white cells. A haemagglutinin reacting with HL-A10 positive donors was found in some multispecific antisera. All these groups show weaker reactions with red cells than with lymphocytes.

Association of white cell and red cell antibodies in human sera

“…We believe that in a larger series the expected correlations might well be found, especially in view of the more recent work of Morton, Pickles, Sutton, and Skov (1971). The collection of the several hundred sera containing both red and white cell antibodies which we believe to be necessary could best be achieved by a blood transfusion or other centre having access to a large number of antenatal sera. )…”

Cell membrane receptors for serological reagents.

“…The red cell antigens Bga, Bgb and Bgc are probably identical with, or very similar to, the HL-A antigens 7, W17 and W28, respectively (Morton, Pickles, and Sutton, 1969;Morton et al, 1971 Most clinical studies of HL-A antigens have been made on material obtained from cells by means of detergents, enzymic digestion or ultrasonic wave bombardment. These methods are harsh and may produce a heterogenous population of proteins so that purification is difficult.…”