Identification of furostanol glycoside 26-&lt;i&gt;O&lt;/i&gt;-β-glucosidase involved in steroidal saponin biosynthesis from &lt;i&gt;Dioscorea esculenta&lt;/i&gt;

Nakayasu, Masaru; Kawasaki, Takashi; Lee, Hyoung Jae; Sugimoto, Yukihiro; Onjo, Michio; Muranaka, Toshiya; Mizutani, Masaharu

doi:10.5511/plantbiotechnology.15.1023b

Cited by 18 publications

(11 citation statements)

References 27 publications

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“…In addition to CYP enzymes, a specific C-26 glucosyltransferase and a 26- O -β-glucosidase are proposed to catalyze the reactions which facilitate the closure of diosgenin ring E and ring F (Scheme 1) [20,26,32]. Within the UGT unigenes with a length above 1000 bps in the T. foenum-graecum transcriptome, there were 21 UGT unigenes that were significantly induced by the MeJA elicitation (Table S3).…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, there were 11 glucosidase annotated unigenes with a length above 1000 bps that were induced by the MeJA elicitation (Table S4). A phylogenetic analysis showed that the candidates of cluster-2140.60643 and cluster-2140.23403 showed a relatively closer relationship with the isoflavone β-glucosidase from Glycine max [33] and the furostanol glycoside 26- O -β-glucosidases from Dioscorea esculenta [20] and Costus speciosus [19] (Figure S5), suggesting that they may be the C-26-β-glucosidase candidates during diosgenin biosynthesis.…”

Section: Resultsmentioning

confidence: 99%

“…In view of the molecular structure of diosgenin, multiple oxidations at the C-22, C-16 and C-26 positions of cholesterol are required [14], which are possibly mediated by specific cytochrome P450s (CYPs). Based on the previous reports [13,19,20], in addition to the sequential oxidation reactions, the addition of glucosyl moiety to the C-26 hydroxyl group followed by the cleavage of this glucosyl bond would be needed to form the F ring of diosgenin (Scheme 1). Thus, a UDP-glucosyltransferase (UGT) in catalyzing the glucosyl transfer reaction and a β-glucosidase in removing the glucosyl unit might be involved in disosgenin biosynthesis as well (Scheme 1).…”

Section: Introductionmentioning

confidence: 99%

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Comparative Transcriptome Analysis Identifies Genes Involved in Diosgenin Biosynthesis in Trigonella foenum-graecum L.

Zhou

et al. 2019

Molecules

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Trigonella foenum-graecum L. (fenugreek) is a valuable resource of producing diosgenin which serves as a substrate for synthesizing more than two hundred kinds of steroidal drugs. Phytochemical analysis indicated that methyl jasmonate (MeJA) efficiently induced diosgenin biosynthesis in fenugreek seedlings. Though early steps up to cholesterol have recently been elucidated in plants, cytochrome P450 (CYP)- and glycosyltransferase (GT)-encoding genes involved in the late steps from cholesterol to diosgenin remain unknown. This study established comparative fenugreek transcriptome datasets from the MeJA-treated seedlings and the corresponding control lines. Differential gene expression analysis identified a number of MeJA-induced CYP and GT candidate genes. Further gene expression pattern analysis across a different MeJA-treating time points, together with a phylogenetic analysis, suggested specific family members of CYPs and GTs that may participate in the late steps during diosgenin biosynthesis. MeJA-induced transcription factors (TFs) that may play regulatory roles in diosgenin biosynthesis were also discussed. This study provided a valuable genetic resource to functionally characterize the genes involved in diosgenin biosynthesis, which will push forward the production of diosgenin in microbial organisms using a promising synthetic biology strategy.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Transcriptome Analysis Identifies Genes Involved in Diosgenin Biosynthesis in Trigonella foenum-graecum L.

Zhou

et al. 2019

Molecules

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“…The biochemical characterization of DeF26G revealed that it was a furostanol glycoside 26-O-β-glucosidase that was involved in the conversion of protodioscin (furostanol saponin) to dioscin (spirostanol saponin). The expression of the DeF26G1 gene in the leaves of Dioscorea esculenta was higher than that in the tubers (Nakayasu et al 2015). Using Illumina deep sequencing Dioscorea composita was used to analyze the transcriptome of 18-month-old Dioscorea composita.…”

Section: Dioscorea Speciesmentioning

confidence: 99%

Recent advances in steroidal saponins biosynthesis and in vitro production

Upadhyay

Jeena

Shikha

et al. 2018

Planta

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Main conclusion Steroidal saponins exhibited numerous pharmacological activities due to the modification of their backbone by different cytochrome P450s (P450) and UDP glycosyltransferases (UGTs). Plant-derived steroidal saponins are not sufficient for utilizing them for commercial purpose so in vitro production of saponin by tissue culture, root culture, embryo culture, etc, is necessary for its large-scale production.Saponin glycosides are the important class of plant secondary metabolites, which consists of either steroidal or terpenoidal backbone. Due to the existence of a wide range of medicinal properties, saponin glycosides are pharmacologically very important. This review is focused on important medicinal properties of steroidal saponin, its occurrence, and biosynthesis. In addition to this, some recently identified plants containing steroidal saponins in different parts were summarized. The high throughput transcriptome sequencing approach elaborates our understanding related to the secondary metabolic pathway and its regulation even in the absence of adequate genomic information of non-model plants. The aim of this review is to encapsulate the information related to applications of steroidal saponin and its biosynthetic enzymes specially P450s and UGTs that are involved at later stage modifications of saponin backbone. Lastly, we discussed the in vitro production of steroidal saponin as the plant-based production of saponin is time-consuming and yield a limited amount of saponins. A large amount of plant material has been used to increase the production of steroidal saponin by employing in vitro culture technique, which has received a lot of attention in past two decades and provides a way to conserve medicinal plants as well as to escape them for being endangered.

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“…Recently, we have seen little progress on advanced molecular studies of Dioscorea. Nakayasu et al [195] have performed comparative transcriptome analysis of high saponin containing yams i.e. D. esculenta and D. cayenensis, and a low saponin containing, D. alata, for understanding biosynthesis of steroidal saponins and identified the β-glucosidase (DeF26G1) gene to be responsible for higher accumulation of saponins in D. esculenta.…”

Section: Nutritional Composition Of Dioscorea (Yam) a Neglected Staple Tuber Crop Of The Tribal Communitiesmentioning

confidence: 99%

Linking Omics and Gene Editing Tools for Rapid Improvement of Traditional Food Plants for Diversified Foods and Sustainable Food Security

Kumar¹,

Anju²,

Kumar³

et al. 2021

Preprint

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The indigenous communities across the globe especially in the rural areas consume locally available plants known as Traditional Food Plants (TFPs) for their nutritional and health-related needs. Recent research shows that many of the traditional food plants are highly nutritious as they contain health beneficial metabolites, vitamins, mineral elements and other nutrients. Excessive reliance on the mainstream staple crops has its own disadvantages. TFPs are nowadays considered important crops of the future and can act as supplementary foods for the burgeoning global population. They can also act as emergency foods in times of pandemics and other situations like COVID-19. The current situation necessitates locally available alternative nutritious TFPs for sustainable food production. To increase the cultivation or improve the traits in TFPs, it is essential to understand the molecular basis of the genes that regulate some important traits such as nutritional components and resilience to biotic and abiotic stresses. The integrated use of modern omics and gene editing technologies provide great opportunities to better understand the genetic and molecular basis of superior nutrient content, climate-resilient traits and adaptation to local agroclimatic zones. Recently, realising the importance and benefits of TFPs, scientists have shown interest in the prospection and sequencing of traditional food plants for their improvements, further cultivation and mainstreaming. Integrated omics such as genomics, transcriptomics, proteomics, metabolomics and ionomics are successfully used in plants and have provided a comprehensive understanding of gene-protein-metabolite networks. Combined use of omics and editing tools has led to successful editing of beneficial traits in few TFPs. This suggests that there is ample scope of integrated use of modern omics and editing tools/techniques for improvement of TFPs and their use for sustainable food production. In this article, we highlight the importance, scope and progress towards improvement of TFPs for valuable traits by integrated use of omics and gene editing techniques.

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Identification of furostanol glycoside 26-<i>O</i>-β-glucosidase involved in steroidal saponin biosynthesis from <i>Dioscorea esculenta</i>

Cited by 18 publications

References 27 publications

Comparative Transcriptome Analysis Identifies Genes Involved in Diosgenin Biosynthesis in Trigonella foenum-graecum L.

Comparative Transcriptome Analysis Identifies Genes Involved in Diosgenin Biosynthesis in Trigonella foenum-graecum L.

Recent advances in steroidal saponins biosynthesis and in vitro production

Linking Omics and Gene Editing Tools for Rapid Improvement of Traditional Food Plants for Diversified Foods and Sustainable Food Security

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