Identification of fungal trehalose for the diagnosis of invasive candidiasis by mass spectrometry

Mery, Alexandre; Jawhara, Samir; François, Nadine; Cornu, Marjorie; Poissy, Julien; Martínez‐Esparza, María; Poulain, D.; Sendid, Boualem; Guérardel, Yann

doi:10.1016/j.bbagen.2022.130083

Cited by 4 publications

(2 citation statements)

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“…The detection of IgG antibodies against enolase has already been reported (see LFA section), and it will be interesting to see if these other potential targets are exploited in the development of future assays [41]. Mass spectrometry has also been used to identify fungal trehalose in the serum of patients to aid the diagnosis of IC, generating positive results in all five candidaemic, BDG-positive patients, whereas mannan antigen ELISA testing was negative in two patients [84].…”

Section: Detection Of Antibodiesmentioning

confidence: 99%

Developments in Fungal Serology

White

2023

Curr Fungal Infect Rep

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Purpose of Review The true incidence of fungal disease is hampered by conventionally poor diagnostic tests, limited access to advanced diagnostics, and limited surveillance. The availability of serological testing has been available for over two decades and generally underpins the modern diagnosis of the most common forms of fungal disease. This review will focus on technical developments of serological tests for the diagnosis of fungal disease, describing advances in clinical performance when available. Recent Findings Despite their longevity, technical, clinical, and performance limitations remain, and tests specific for fungal pathogens outside the main pathogens are lacking. The availability of LFA and automated systems, capable of running multiple different tests, represents significant developments, but clinical performance data is variable and limited. Summary Fungal serology has significantly advanced the diagnosis of the main fungal infections, with LFA availability increasing accessibility to testing. Combination testing has the potential to overcome performance limitations.

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Section: Detection Of Antibodiesmentioning

confidence: 99%

Developments in Fungal Serology

White

2023

Curr Fungal Infect Rep

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“…In nature, trehalose is a non-reducing disaccharide that is linked to the glucose residue by an α-1,1 glycoside bond ( Trevelyan and Harrison, 1956 ; Mery et al, 2022 ). It is an excellent natural desiccant and preservative, as well as a new functional oligosaccharide ( De Britto et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

In vitro assembly of the trehalose bi-enzyme complex with artificial scaffold protein

Wang,

Jiang,

Liu

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: Trehalose is a significant rare sugar known for its stable properties and ability to protect biomolecules from environmental factors.Methods: In this study, we present a novel approach utilizing a scaffold protein-mediated assembly method for the formation of a trehalose bi-enzyme complex. This complex consists of maltooligosyltrehalose synthase (MTSase) and maltooligosyltrehalose trehalohydrolase (MTHase), which work in tandem to catalyze the substrate and enhance the overall catalytic efficiency. Utilizing the specific interaction between cohesin and dockerin, this study presents the implementation of an assembly, an analysis of its efficiency, and an exploration of strategies to enhance enzyme utilization through the construction of a bi-enzyme complex under optimal conditions in vitro.Results and Discussion: The bi-enzyme complex demonstrated a trehalose production level 1.5 times higher than that of the free enzyme mixture at 40 h, with a sustained upward trend. Compared to free enzyme mixtures, the adoption of a scaffold protein-mediated bi-enzyme complex may improve cascade reactions and catalytic effects, thus presenting promising prospects.

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Harvey

2024

Mass Spectrometry Reviews

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The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022. As with previous review, this review also includes a few papers that describe methods appropriate to analysis by MALDI, such as sample preparation, even though the ionization method is not MALDI. The review follows the same format as previous reviews. It is divided into three sections: (1) general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, quantification and the use of computer software for structural identification. (2) Applications to various structural types such as oligo‐ and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals, and (3) other general areas such as medicine, industrial processes, natural products and glycan synthesis where MALDI is extensively used. Much of the material relating to applications is presented in tabular form. MALDI is still an ideal technique for carbohydrate analysis, particularly in its ability to produce single ions from each analyte and advancements in the technique and range of applications show little sign of diminishing.

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Identification of fungal trehalose for the diagnosis of invasive candidiasis by mass spectrometry

Cited by 4 publications

References 38 publications

Developments in Fungal Serology

Developments in Fungal Serology

In vitro assembly of the trehalose bi-enzyme complex with artificial scaffold protein

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

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