Identification of functional α2- and β-adrenergic receptors in mammalian spermatozoa

Adeoya-Osiguwa, Susan; Gibbons, Rachel; Fraser, Lynn R.

doi:10.1093/humrep/del016

Cited by 39 publications

(45 citation statements)

References 26 publications

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“…The mechanism involved in lipid catabolism is that ZAG can stimulate lipolysis in adipocytes through the b 3 -adrenoreceptor, which directly results in the stimulation of adenylate cyclase and an increase in intracellular cAMP (Russell et al 2004, Tisdale 2004, Russell & Tisdale 2005. Interestingly, b-adrenergic receptors have already been detected in human sperm (Adeoya-Osiguwa & Fraser 2005, Adeoya-Osiguwa et al 2006, and b 3 -adrenoreceptor also has a similar localization to ZAG. Thus, we presume that ZAG is able to stimulate cAMP signaling via the b-adrenoreceptor in human sperm, a potential pathway of motility regulation.…”

Section: Discussionmentioning

confidence: 99%

The role of Zn-α2 glycoprotein in sperm motility is mediated by changes in cyclic AMP

Qü¹,

Ying²,

Guo³

et al. 2007

Reproduction

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Sperm motility is essential for male reproduction or natural fertilization. The cyclic AMP (cAMP)/cAMP-dependent protein kinase A (PKA) signaling pathway is generally recognized as one of the significant signaling pathways in the regulation of mammalian spermatozoan motility. Since Zn-a2-glycoprotein (ZAG) activity in mammalian adipose tissue is mediated via the b 3 -adrenoreceptor, with upregulation of the cAMP pathway, we hypothesize that ZAG may play the same role in sperm motility regulation, a new factor of regulation of sperm motility. Therefore, the gene encoding human ZAG was cloned and polyclonal antibodies were generated, and then laser scanning confocal microscopy and flow cytometry were employed to identify this protein in human spermatozoa. The results showed that ZAG protein was mostly localized on the pre-equatorial region covering the acrosome, neck, and middle piece of the flagellum of spermatozoa. Furthermore, using computer-assisted sperm analysis, we found that anti-human ZAG antibodies could significantly reduce the motility of human swim-up spermatozoa after 90-or 120-min incubation (P!0.05 and P!0.01 respectively), together with the decreasing of intracellular cAMP and PKA levels. In conclusion, these data suggest that ZAG is present in human spermatozoa and may be involved in the regulation of sperm motility via the cAMP/PKA signaling pathway.

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Section: Discussionmentioning

confidence: 99%

The role of Zn-α2 glycoprotein in sperm motility is mediated by changes in cyclic AMP

Qü¹,

Ying²,

Guo³

et al. 2007

Reproduction

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“…Zinc is mostly present in sperm flagella and its concentration is negatively correlated with to sperm motility and velocity (Henkel et al 1999); its chelation oxidizes the sulfhydryl groups to disulfide bridges that stabilize and stiffen the outer dense fibers in flagellum (Calvin HI et al 1974), leading to increase in straight line velocity and progressive motility (Wroblewski et al 2003) and extending the fertile life span of bovine frozen-thawed spermatozoa (Pavlok 2000). Epinephrine modulates cAMP production, which stimulates sperm capacitation (Adeoya-Osiguwa et al 2006) and even capacitates sperm in presence of zinc chelators (Way and Killian 2002). Meanwhile, high dose of PHE (75µl/ml PHE), equivalent for (37.7µM penicillamin, 18.9 µM hypotaurine and 3µM epinephrine) didn't lead to improvement in fertilization and cleavage rates when compared to control.…”

Section: Resultsmentioning

confidence: 99%

“…Meanwhile, high dose of PHE (75µl/ml PHE), equivalent for (37.7µM penicillamin, 18.9 µM hypotaurine and 3µM epinephrine) didn't lead to improvement in fertilization and cleavage rates when compared to control. This observation can be explained by epinephrine's capacitation inhibitory effect on the already capacitated sperm as the spermatozoa after capacitation already have α receptors (AdeoyaOsiguwa andFraser 2005, andAdeoya-Osiguwa et al 2006). …”

Section: Resultsmentioning

confidence: 99%

Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa

ElRuby¹,

Hussein²,

Bedier³

et al. 2017

AJVS

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Key words: D-Penecillamine, Hypotaurine, Epinephrine, In vitro fertilization, BuffaloIn vitro fertilization (IVF) is a necessary procedure in the in vitro production of embryo. Spermatozoa need to undergo capacitation to be able to perform the acrosome reaction which is required for zona pellucida penetration. Different additives are used to aid in that process, such as caffeine and theophylline. In this study, the effects of IVF media supplementation with D-penicillamine, Hypotaurine and epinephrine (PHE) on fertilization and cleavage rates were assessed. Cumulus oocyte complexes (COCs) containing a compact cumulus cells and normal homogenous cytoplasm were selected. In vitro maturation (IVM) was performed in TCM199 supplied with hormones overnight in a culture conditions of 38.5°C and 5% CO 2 in air. After 20-22 hr. of maturation, oocytes were randomly assigned to five groups of sperm-TALP media (control and four different PHE concentrations) then inseminated. The fertilization rate of the expected embryos were assessed after 22 hr. after staining with aceto-orcin 1% or cultured for 48 hr. to estimate the cleavage rate. The current data revealed that, 25 µl/ml PHE significantly (P< 0.05) improved fertilization (55.93 vs 36.96%) and cleavage (46.15 vs 22.22%) rates when compared to control.

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“…Increased fluid formation, effects on Cl K ion transport and electrical potential differences have been shown after treatment of rabbit oviductal cells with agonists of adrenergic receptors (Dickens et al 1993, Dickens & Leese 1994. Besides, the effects on the oviduct epithelium, adrenoceptor agonists can also influence the functioning of spermatozoa in the oviduct, including capacitation and acrosome reaction (Way & Killian 2002, Adeoya-Osiguwa et al 2006.…”

Section: Discussionmentioning

confidence: 99%

Expression of adrenergic receptors in mouse preimplantation embryos and ovulated oocytes

Číkoš

Rehák²,

Czikková

et al. 2007

Reproduction

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Epinephrine and norepinephrine can play an important role in basic developmental processes such as embryogenesis and morphogenesis, regulating cell proliferation, differentiation and migration. We showed that b-adrenergic receptors can mediate the effects of catecholamines on preimplantation embryos in our previous work. In the present study, we designed specific oligonucleotide primers which can distinguish among all members of the a-adrenergic receptor family, and showed (using RT-PCR) that the a2C-adrenergic receptor is transcribed in ovulated oocytes, 8-to 16-cell morulae and expanded blastocysts. We did not detect the a2C-adrenoceptor transcript in 4-cell embryos. Our immunohistochemical study showed the presence of a-2C-adrenoceptor protein in ovulated oocytes, 8-to 16-cell embryos and blastocysts, but the signal in 4-cell embryos was weak, and probably represents remaining protein of maternal origin. We did not detect any other a-adrenergic receptor in preimplantation embryos and oocytes. Exposure of mouse preimplantation embryos to the a2-adrenergic agonist UK 14 304 led to significant reduction of the embryo cell number, and the effect was dose dependent. Our results suggest that epinephrine and norepinephrine could affect the embryo development in the oviduct via adrenergic receptors directly and support the opinion that maternal stress can influence the embryo even in very early pregnancy.

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Identification of functional α2- and β-adrenergic receptors in mammalian spermatozoa

Cited by 39 publications

References 26 publications

The role of Zn-α2 glycoprotein in sperm motility is mediated by changes in cyclic AMP

The role of Zn-α2 glycoprotein in sperm motility is mediated by changes in cyclic AMP

Improvement of in Vitro Fertilization in Buffalo by Increasing the Fertilizing Capacity of Spermatozoa

Expression of adrenergic receptors in mouse preimplantation embryos and ovulated oocytes

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