Identification of Functional Patterns of Androgenetic Alopecia Using Transcriptome Profiling in Distinct Locations of Hair Follicles

Miao, Yong; Qu, Qian; Jiang, Wei; Liu, Xiaomin; Shi, Panli; Fan, Zhexiang; Du, Lijuan; Wang, Gaofeng; Liu, Xiao‐Nan; Guo, Zhaoze; Liu, Yang; Liu, Fang; Liu, Ya-Rui

doi:10.1016/j.jid.2017.10.027

Cited by 14 publications

(8 citation statements)

References 10 publications

(8 reference statements)

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“…A previous microarray-seq identified CXCL10 and CXCL11 were functionally important transcriptional target genes of Eda involved in hair follicle spacing ( Lefebvre et al, 2012 ). Other DEG, such as FOS, NOTCH2 and NOTCH3, were also found related to follicle hair cycling, take part in MAPK, and notch signaling pathways ( Miao et al, 2018 ; Zhang et al., 2020a ; Zhang et al., 2020b ). Above all, we systematically identified the lncRNAs and mRNAs involved in Tibetan cashmere goats skin tissues.…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of lncRNA and mRNA reveals novel insights into cashmere fineness in Tibetan cashmere goats

Zhao

Tian

et al. 2020

PeerJ

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Tibetan cashmere goats are famous for producing the finest, softest and lightest cashmere fiber in China. The growth and development of skin are closely related to fineness and are the key factors affecting the quality of cashmere. To investigate the specific role of long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) in regulating cashmere fineness of Tibetan Cashmere goats in the anagen phase, we conducted high-throughput RNA sequencing of fine-type and coarse-type skin tissues. We identified 2,059 lncRNA candidates (1,589 lncRNAs annotated, 470 lncRNAs novel), and 80 differentially expressed (DE) lncRNAs and their potential targets were predicted. We also identified 384 DE messenger RNAs (mRNAs) out of 29,119 mRNAs. Several key genes in KRT26, KRT28, KRT39, IFT88, JAK3, NOTCH2 and NOTCH3 and a series of lncRNAs, including ENSCHIT00000009853, MSTRG.16794.17, MSTRG.17532.2, were shown to be potentially important for regulating cashmere fineness. GO and KEGG enrichment analyses of DE mRNAs and DE lncRNAs targets significantly enriched in positive regulation of the canonical Wnt signaling pathway, regulation of protein processing and metabolism processes. The mRNA-mRNA and lncRNA-mRNA regulatory networks further revealed potential transcripts involved in cashmere fineness. We further validated the expression patterns of DE mRNAs and DE lncRNAs by quantitative real-time PCR (qRT-PCR), and the results were consistent with the sequencing data. This study will shed new light on selective cashmere goat breeding, and these lncRNAs and mRNAs that were found to be enriched in Capra hircus RNA database.

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Section: Discussionmentioning

confidence: 99%

Integrated analysis of lncRNA and mRNA reveals novel insights into cashmere fineness in Tibetan cashmere goats

Zhao

Tian

et al. 2020

PeerJ

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“…Assays using HFs obtained through plucking, follicular unit extraction (FUE), and skin biopsies have been performed. Through a comprehensive literature search, we found 9 studies involving human HGs [ 144 , 145 , 146 , 147 , 148 , 149 , 150 , 151 ]. In most studies (67%), the complete HF was used to obtain mRNA samples.…”

Section: Transcription Profiling Studies Of Agamentioning

confidence: 99%

The Biology and Genomics of Human Hair Follicles: A Focus on Androgenetic Alopecia

Cuevas-Diaz Duran,

Martinez-Ledesma,

Garcia-Garcia

et al. 2024

IJMS

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Androgenetic alopecia is a highly prevalent condition mainly affecting men. This complex trait is related to aging and genetics; however, multiple other factors, for example, lifestyle, are also involved. Despite its prevalence, the underlying biology of androgenetic alopecia remains elusive, and thus advances in its treatment have been hindered. Herein, we review the functional anatomy of hair follicles and the cell signaling events that play a role in follicle cycling. We also discuss the pathology of androgenetic alopecia and the known molecular mechanisms underlying this condition. Additionally, we describe studies comparing the transcriptional differences in hair follicles between balding and non-balding scalp regions. Given the genetic contribution, we also discuss the most significant risk variants found to be associated with androgenetic alopecia. A more comprehensive understanding of this pathology may be generated through using multi-omics approaches.

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“…The library preparations were sequenced on an Illumina Hiseq 2500 platform and the details of library construction and sequencing are the same as in our previous study (Miao et al, 2018).…”

Section: Rna Library Construction and Sequencingmentioning

confidence: 99%

Emerging Role of Dermal White Adipose Tissue in Modulating Hair Follicle Development During Aging

Chen

Fan

Zhu

et al. 2021

Front. Cell Dev. Biol.

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Hair follicle stem cells are extensively reprogrammed by the aging process, manifesting as diminished self-renewal and delayed responsiveness to activating cues, orchestrated by both intrinsic microenvironmental and extrinsic macroenvironmental regulators. Dermal white adipose tissue (dWAT) is one of the peripheral tissues directly adjacent to hair follicles (HFs) and acts as a critical macroenvironmental niche of HF. dWAT directly contributes to HF aging by paracrine signal secretion. However, the altered interrelationship between dWAT and HF with aging has not been thoroughly understood. Here, through microdissection, we separated dWAT from the skin of aged mice (18 months) and young mice (2 months) in telogen and depilation-induced anagen for transcriptome comparing. Notably, compared with young dWAT, aberrant inflammatory regulators were recapitulated in aging dWAT in telogen, including substantial overexpressed inflammatory cytokines, matrix metalloproteinases, and prostaglandin members. Nonetheless, with anagen initiation, inflammation programs were mostly abolished in aging dWAT, and instead of which, impaired collagen biosynthesis, angiogenesis, and melanin synthesis were identified. Furthermore, we confirmed the inhibitory effect on hair growth of CXCL1, one of the most significantly upregulated inflammation cytokines in aging dWAT. Besides this, we also identified the under-expressed genes related to Wnt signaling fibroblast growth factor family members and increased BMP signaling in aging dWAT, further unraveling the emerging role of dWAT in aging HFs malfunction. Finally, we proved that relieving inflammation of aging dWAT by injecting high-level veratric acid stimulated HF regenerative behavior in aged mice. Concomitantly, significantly decreased TNF-a, CCL2, IL-5, CSF2, and increased IL10 in dWAT was identified. Overall, the results elaborated on the complex physiological cycling changes of dWAT during aging, providing a basis for the potential regulatory effect of dWAT on aging HFs.

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Identification of Functional Patterns of Androgenetic Alopecia Using Transcriptome Profiling in Distinct Locations of Hair Follicles

Abstract: A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages. J Invest Dermatol 2001;117:3e15. Paus R, Foitzik K. In search of the "hair cycle clock": a guided tour. Differentiation 2004;72: 489e511.

Cited by 14 publications

References 10 publications

Integrated analysis of lncRNA and mRNA reveals novel insights into cashmere fineness in Tibetan cashmere goats

Integrated analysis of lncRNA and mRNA reveals novel insights into cashmere fineness in Tibetan cashmere goats

The Biology and Genomics of Human Hair Follicles: A Focus on Androgenetic Alopecia

Emerging Role of Dermal White Adipose Tissue in Modulating Hair Follicle Development During Aging

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