Identification of full-length circular nucleic acids using long-read sequencing technologies

Lu, Wenzhou; Yu, Kequan; Li, Xiaohan; Ge, Qinyu; Liang, Geyu; Bai, Ya Mei

doi:10.1039/d1an01147b

Cited by 1 publication

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“…In addition, nanopore sequencing can detect and quantify low abundance circRNAs, which is an important aspect as many circRNAs are present at low levels in biological samples (Lu et al, 2021). Full‐length reconstruction has been accomplished using Illumina and Nanopore RNA‐seq datasets (Fuchs et al, 2022).…”

Section: Full‐length Approaches For Circrna Assessmentmentioning

confidence: 99%

Current advances in circular RNA detection and investigation methods: Are we running in circles?

Drula,

Braicu,

Berindan‐Neagoe

2024

WIREs RNA

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Circular RNAs (circRNAs), characterized by their closed‐loop structure, have emerged as significant transcriptomic regulators, with roles spanning from microRNA sponging to modulation of gene expression and potential peptide coding. The discovery and functional analysis of circRNAs have been propelled by advancements in both experimental and bioinformatics tools, yet the field grapples with challenges related to their detection, isoform diversity, and accurate quantification. This review navigates through the evolution of circRNA research methodologies, from early detection techniques to current state‐of‐the‐art approaches that offer comprehensive insights into circRNA biology. We examine the limitations of existing methods, particularly the difficulty in differentiating circRNA isoforms and distinguishing circRNAs from their linear counterparts. A critical evaluation of various bioinformatics tools and novel experimental strategies is presented, emphasizing the need for integrated approaches to enhance our understanding and interpretation of circRNA functions. Our insights underscore the dynamic and rapidly advancing nature of circRNA research, highlighting the ongoing development of analytical frameworks designed to address the complexity of circRNAs and facilitate the assessment of their clinical utility. As such, this comprehensive overview aims to catalyze further advancements in circRNA study, fostering a deeper understanding of their roles in cellular processes and potential implications in disease.This article is categorized under: RNA Methods > RNA Nanotechnology RNA Methods > RNA Analyses in Cells RNA Methods > RNA Analyses In Vitro and In Silico

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Section: Full‐length Approaches For Circrna Assessmentmentioning

confidence: 99%

Current advances in circular RNA detection and investigation methods: Are we running in circles?

Drula,

Braicu,

Berindan‐Neagoe

2024

WIREs RNA

View full text Add to dashboard Cite

show abstract

Identification of full-length circular nucleic acids using long-read sequencing technologies

Abstract: Unlike the traditional perception in genomic DNA or linear RNA, circular nucleic acids are a class of functional biomolecules with a circular configuration and are often observed in nature. These...

Cited by 1 publication

References 78 publications

Current advances in circular RNA detection and investigation methods: Are we running in circles?

Current advances in circular RNA detection and investigation methods: Are we running in circles?

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