Identification of forensically significant calliphorids based on mitochondrial DNA cytochrome oxidase I (COI) gene in China

Liu, Qinlai; Cai, Jifeng; Guo, Yadong; Wang, Xinghua; Gu, Yan; Wen, Ji; Meng, Fanming; Yi, Wenping

doi:10.1016/j.forsciint.2011.02.004

Cited by 8 publications

(5 citation statements)

References 12 publications

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“…Details of the primers and PCR condition were described in previous studies (26,27). Gel electrophoresis was used to isolate PCR products, which were then purified using QiaQuick PCR Purification Kit (Qiagen, Germantown, MD, USA).…”

Section: Methodsmentioning

confidence: 99%

Reliability of long vs short COI markers in identification of forensically important flies

Aly¹

2014

Croat Med J

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AimTo compare the reliability of short and long cytochrome oxidase I gene fragment (COI) in identification of forensically important Diptera from Egypt and China.MethodsWe analyzed 50 specimens belonging to 18 species. The two investigated markers were amplified by polymerase chain reaction (PCR) followed by direct sequencing. Nucleotide sequence divergences were calculated using the Kimura two-parameter (K2P) distance model and neighbor-joining (NJ) phylogenetic trees.ResultsAlthough both tested fragments showed an overlap between intra and interspecific variations, long marker had greater completeness of monophyletic separation with high bootstrap support. Moreover, NJ tree based on the long fragment clustered species more in accordance with their taxonomic classification than that based on the short fragment.ConclusionIn dipterous identification, it is recommended to use the long COI marker due to its greater reliability and safety.

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Section: Methodsmentioning

confidence: 99%

Reliability of long vs short COI markers in identification of forensically important flies

Aly¹

2014

Croat Med J

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“…Among these markers, COI and COII have been predominantly used in forensic entomology (Sperling et al 1994, Malgorn and Coquoz 1999, Vincent et al 2000, Wallman and Donnellan 2001, Wells et al 2001, Wells and Sperling 2001, Harvey et al 2003, 2008, Wells and Stevens 2008, Liu et al 2011, Boehme et al 2012, Jordaens et al 2012, Renaud et al 2012). Coincidentally, a fragment of the 5’ end of COI has been selected as the standard barcode marker for animal identification by the Consortium for the Barcode of Life (Hebert et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Utility of GenBank and the Barcode of Life Data Systems (BOLD) for the identification of forensically important Diptera from Belgium and France

Sonet¹,

Jordaens²,

Braet³

et al. 2013

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Fly larvae living on dead corpses can be used to estimate post-mortem intervals. The identification of these flies is decisive in forensic casework and can be facilitated by using DNA barcodes provided that a representative and comprehensive reference library of DNA barcodes is available.We constructed a local (Belgium and France) reference library of 85 sequences of the COI DNA barcode fragment (mitochondrial cytochrome c oxidase subunit I gene), from 16 fly species of forensic interest (Calliphoridae, Muscidae, Fanniidae). This library was then used to evaluate the ability of two public libraries (GenBank and the Barcode of Life Data Systems – BOLD) to identify specimens from Belgian and French forensic cases. The public libraries indeed allow a correct identification of most specimens. Yet, some of the identifications remain ambiguous and some forensically important fly species are not, or insufficiently, represented in the reference libraries. Several search options offered by GenBank and BOLD can be used to further improve the identifications obtained from both libraries using DNA barcodes.

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“…A portion of 272 bp fragment of the mitochondrial COI gene was amplified and sequenced using forward primer (5′‐CAGATCGAAATTTAAATAC‐TTC‐3.′) and reverse primers (5.′‐GTAT‐ CAACATCTATTCCTAC‐3′), details of the primers were described by Liu et al (2011).…”

Section: Methodsmentioning

confidence: 99%

“…With the development of the molecular identification method, more and more discussions about the feature of the mitochondrial DNA (mtDNA) fragments to the efficiency of identification, such as the length of the fragments, were raised and discussed. The COI gene of different lengths and regions has been studied, and several short fragments were proven to have sufficient discrimination power (Harvey et al , 2003; Zehner et al , 2004; Saigusa et al , 2005; Ames et al , 2006; Desmyter & Gosselin, 2009; Liu et al , 2011), which makes it in particular suitable for forensic applications. However, some closely related species failed to be discriminated by COI sequence analysis (Wallman & Donnellan, 2001; Harvey et al , 2003; Wells et al , 2004, 2007).…”

Section: Introductionmentioning

confidence: 99%

Identification of forensically important flesh flies based on a shorter fragment of the cytochrome oxidase subunit I gene in China

Guo

Cai

Meng

et al. 2011

Medical Vet Entomology

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With the development of molecular identification, there has been a great deal of discussion about the feature of the mitochondrial DNA (mtDNA) fragments. Although longer fragments may minimize stochastic variation across taxa and be more likely to reflect broader patterns of nucleotide divergence, shorter fragments have many advantages, such as quick, easy and economical. Extensive application of long mtDNA segments for species identification cannot always be achieved as a result of constraints in time and money. In the present study, a molecular identification method involving the sequencing of a 272-bp 'barcode' fragment of the mitochondrial cytochrome oxidase subunit I (COI) gene from 55 specimens, representing 7 Chinese sarcophagid species from varying populations, was evaluated. Phylogenetic analysis of the sequenced segments showed that all sarcophagid specimens were properly assigned into seven species, which indicated the possibility of separation congeneric species with the short fragments. The results of this research will be instrumental for the implementation of the Chinese Sarcophagidae database.

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Identification of forensically significant calliphorids based on mitochondrial DNA cytochrome oxidase I (COI) gene in China

Cited by 8 publications

References 12 publications

Reliability of long vs short COI markers in identification of forensically important flies

Reliability of long vs short COI markers in identification of forensically important flies

Utility of GenBank and the Barcode of Life Data Systems (BOLD) for the identification of forensically important Diptera from Belgium and France

Identification of forensically important flesh flies based on a shorter fragment of the cytochrome oxidase subunit I gene in China

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