Identification of flooding stress responsible cascades in root and hypocotyl of soybean using proteome analysis

Komatsu, Setsuko; Sugimoto, Tatsuho; Hoshino, Tomoki; Nanjo, Yohei; Furukawa, Kiyoshi

doi:10.1007/s00726-009-0277-0

Cited by 71 publications

(64 citation statements)

References 37 publications

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“…The seedlings inevitably show anaerobic pathways in which they generate ATP through glycolysis and they regenerate NAD + through ethanol fermentation. Several studies confirmed that the expression of proteins involved in glycolysis and fermentation pathways, such as UDP-glucose pyrophosphorylase, fructose-bisphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase, and alcohol dehydrogenase are upregulated in response to flooding stress (Alam et al, 2010a;Hashiguchi et al, 2009;Komatsu et al, 2010b;Nanjo et al, 2010). These results confirm that flooding stress also involves stress from oxygen starvation.…”

Section: Floodingsupporting

confidence: 70%

Proteomics Approach for Identifying Abiotic Stress Responsive Proteins in Soybean

Nouri¹,

Toorchi²,

Komatsu³

2011

Soybean - Molecular Aspects of Breeding

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Section: Floodingsupporting

confidence: 70%

Proteomics Approach for Identifying Abiotic Stress Responsive Proteins in Soybean

Nouri¹,

Toorchi²,

Komatsu³

2011

Soybean - Molecular Aspects of Breeding

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“…sis when fermentative processes were induced 60 . Komatsu et al 21 reported that the expression level of glycolysis and fermentation-associated proteins, UDP pyrophosphorylase, fructose-bisphosphate aldolase, and GA3P dehydrogenase were increased under flooding conditions in soybean. In this study, the amount of phosphoenolpyruvate (PEP), pyruvate and lactate, related to glycolysis and fermentation, transiently increased, but decreased 3 to 4 days after flooding (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The causes of flooding injury after radicle protrusion in the soybean, however, have not been well elucidated to date. In our previous report 21 ,the growth of soybean seedlings under flooding conditions was similar to that under anoxia, and the level of protein related to fermentation was greatly upregulated during flooding. Furthermore, Hashiguchi et al 16 and Nanjyo et al 38 demonstrated that flooding of soybean seedlings affected the expression of certain proteins, involved not only in fermentation, but also glycolysis, suggesting that glycolytic and fermentative metabolism were also affected by flooding stress during germination.…”

Section: Metabolite Analysismentioning

confidence: 99%

Evaluation of Metabolite Alteration under Flooding Stress in Soybeans

Nakamura¹,

Yamamoto²,

Hiraga³

et al. 2012

JARQ

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Soybean is a crop known to be susceptible to flooding and enhancing flooding tolerance may be a workable strategy to improve soybean production. To elucidate the effects of flooding on soybean metabolism, metabolite alterations in seedlings during flooding treatment were identified using capillary electrophoresis-mass spectrometry (CE/MS). The principal component analysis (PCA) of soybean seedlings revealed that the first component accounted for 62.2% of total variance, and the alteration of metabolites in control and flooding treatments appears to be separated by this component. Furthermore, comparison of the metabolic loading scores in the first component of PCA show that the significant metabolites for the first component were alanine (Ala), gamma-aminobutyric acid (GABA), citrate, fumarate and malate. Quantitative analysis revealed that the total soluble sugar content of seedlings in both control and flooding treatments had declined and was lower in the latter than the former. Phosphoenolpyruvate, pyruvate and lactate, which belong to glycolytic and fermentation pathways, increased transiently, but decreased 3 to 4 days after treatment. Citrate, 2-oxoglutarate, succinate, fumarate, Ala, and GABA, which are related to the TCA cycle and amino acid metabolism, accumulated during flooding treatment. These results suggest that metabolism associated with the TCA cycle, the Ala synthetic pathway, and the GABA shunt may be strongly influenced by flooding during soybean germination.Discipline: Agricultural environment / Soil fertilizers and plant nutrition Additional key words: capillary electrophoresis -mass spectrometry (CE/MS), flooding, metabolomic analysis, soybean seedlings Present address:

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“…Low oxygen level causes fast changes in gene transcription, protein synthesis and degradation and cellular metabolism (Bailey-Serres and Voesenek, 2008;Komatsu et al, 2009, Komatsu et al, 2010.…”

Section: Discussionmentioning

confidence: 99%

Waterlogging-induced changes in fermentative metabolism in roots and nodules of soybean genotypes

Borella

Amarante

Oliveira

et al. 2014

Sci. agric. (Piracicaba, Braz.)

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Waterlogging blocks the oxygen supply to the root system which inhibits respiration, and greatly reduces the energy status of cells that affect important metabolic processes. This study evaluated fermentative metabolism and carbohydrate contents in the root system of two soybean (Glycine max L. Merril) genotypes under hypoxic and post-hypoxic conditions. Nodulated plants (genotypes Fundacep 53 RR and BRS Macota) were grown in vermiculite and transferred to a hydroponic system at the reproductive stage. The root system was submitted to hypoxia by flowing N 2 (nitrogen) gas in a solution for 24 and 72 h. For recovery, plants returned to normoxia condition by transfer to vermiculite for 24 and 72 h. Fermentative enzyme activity, levels of anaerobic metabolites and carbohydrate content were all quantified in roots and nodules. The activity of alcohol dehydrogenase, pyruvate decarboxylase and lactate dehydrogenase enzymes, as well as the content of ethanol and lactate, increased with hypoxia in roots and nodules, and subsequently returned to pre-hypoxic levels in the recovery phase in both genotypes. Pyruvate content increased in nodules and decreased in roots. Sugar and sucrose levels increased in roots and decreased in nodules under hypoxia in both genotypes. Fundacep RR 53 was more responsive to the metabolic effects caused by hypoxia and post-hypoxia than BRS Macota, and it is likely that these characteristics contribute positively to improving adaptation to oxygen deficiency.Keywords: Glycine max, oxygen deficiency, enzyme activities, anaerobic metabolites, sugars IntroductionSoil waterlogging is a common abiotic stress worldwide in cultivated areas and influences the composition and productivity of soybean (Glycine max L. Merril) and most crops species (Jackson and Colmer, 2005;Githiri et al., 2006;Sairam et al., 2009;Kokubun, 2013). In waterlogged soils, gas exchanges between root systems and soil porous spaces are limited due to oxygen diffusion resistance that is around 10,000 times higher in water than in the air (Armstrong et al., 1994;Dongen et al., 2003;Zabalza et al., 2009;Bailey-Serres et al., 2012).Decrease in the oxygen level is the main factor that causes stress, leading to a chain signaling that unleashes a series of metabolic changes (Horchani et al., 2009), such as N metabolism and interconversion of amino acids (Puiatti and Sodek, 1999;Oliveira et al., 2013), changes in carbohydrates levels and energetic metabolism (Sousa and Sodek, 2002a), in an effort to secure plant survival and growth when exposed to hypoxic stress (Geigenberger, 2003). Anaerobic metabolism is activated under low oxygen concentration and as a consequence, there is a significant decrease in energy production that is derived mainly from glycolysis in contrast to oxidative phosphorylation (Kumutha et al., 2008;Horchani et al., 2009;Sairam et al., 2009;Zabalza et al., 2009). Plant survival under these conditions depends almost exclusively on anaerobic metabolism (Sousa and Sodek, 2002a).Due to the lack of O 2 as the fi...

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Identification of flooding stress responsible cascades in root and hypocotyl of soybean using proteome analysis

Cited by 71 publications

References 37 publications

Proteomics Approach for Identifying Abiotic Stress Responsive Proteins in Soybean

Proteomics Approach for Identifying Abiotic Stress Responsive Proteins in Soybean

Evaluation of Metabolite Alteration under Flooding Stress in Soybeans

Waterlogging-induced changes in fermentative metabolism in roots and nodules of soybean genotypes

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