Identification of Flavonoids in Iraqi Date Palm Pollen by HPLC

Al-Samarrai, Rafah Razooq Hameed; –Samarrai, Abdul-Monaim Hamad Al; Al-Salihi, Ferah Ghali

doi:10.13005/ojc/330252

Cited by 7 publications

(5 citation statements)

References 6 publications

(6 reference statements)

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“…Other flavonoid aglycones were quantified in very low amounts such as luteolin, apigenin, and naringenin. The results obtained in the work of Al-Samarrai et al indicated that date palm pollen has some level of naringin (64.574 mg/kg), and apigenin (109.117 mg/kg) too [60].…”

Section: Resultsmentioning

confidence: 99%

Chemical Fingerprint of ‘Oblačinska’ Sour Cherry (Prunus cerasus L.) Pollen

et al. 2019

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The aim of this research was to analyze sugars and phenolics of pollen obtained from 15 different ‘Oblačinska’ sour cherry clones and to assess the chemical fingerprint of this cultivar. Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography–diode array detector–tandem mass spectrometry (UHPLC-DAD MS/MS) system. Glucose was the most abundant sugar, followed by fructose and sucrose. Some samples had high level of stress sugars, especially trehalose. Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after. According to the principal component analysis (PCA), fructose, maltose, maltotriose, sorbitol, and trehalose were the most important sugars in separating pollen samples. PCA showed splitting off clones VIII/1, IV/8, III/9, and V/P according to the quantity of phenolics and dissimilar profiles. Large differences in chemical composition of studied ‘Oblačinska sour cherry’ clone pollen were shown, proving that it is not a cultivar, but population. Finally, due to the highest level of phenolics, clones IV/8, XV/3, and VIII/1 could be singled out as a promising one for producing functional food and/or in medicinal treatments.

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Section: Resultsmentioning

confidence: 99%

Chemical Fingerprint of ‘Oblačinska’ Sour Cherry (Prunus cerasus L.) Pollen

et al. 2019

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“…apigenin, luteolin, isorhamnetin, apigenin-7-O-β-glucuronide, luteolin-7-O-glucuronide and naringin) and phytosterols ( i.e. stigmasterol) 28 , 29 ( Fig. 1 ).…”

Section: Resultsmentioning

confidence: 99%

“…As mentioned in above sections, studies reported that DPP comprised different classes of natural products including non-flavonoids (especially phenolic acids), flavonoids, 29 amino acids including aspartic, proline, threonine, phenylalanine, alanine, glycine, arginine, methionine, isoleucine, leucine, tyrosine, glutamine, lysine, histidine, valine, and serine, 69 vitamins (B1, B2, B12, A, D, C, E), 68 fatty acids, phytosterols, 68 and mineral and trace elements (e.g. potassium, calcium, sodium, manganese, phosphorous, zinc, iron, selenium).…”

Section: Discussionmentioning

confidence: 96%

“…Polyphenols including isorhamnetin (122.251 µg/g), chlorogenic acid (71.146 µg/g), ferulic acid (99.188 µg/g), naringin (64.574 µg/g), apigenin (109.117 µg/g), apigenin-7-O-β glycopyranoside (48.391 µg/g), luteolin (28.883 µg/g) and luteolin-7-O-β-glucuronide (18.291 µg/g) were the most abundant compounds isolated from DPPs. 29 Generally, food products containing high levels of natural products including flavonoids and phenolic acids, vitamins, minerals and trace elements have considered as “ functional foods ” to provide many health benefits for humans. 81 , 82 …”

Section: Discussionmentioning

confidence: 99%

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Comparative in vitro/theoretical studies on the anti-angiogenic activity of date pollen hydro-alcoholic extract: Highlighting the important roles of its hot polyphenols

et al. 2018

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Introduction: Date palm pollen (DPP) is the male reproductive soft powder from date flowers widely used as the valuable dietary supplement to fortify the size of testis and ovarian to increase the power of sex. This part of date palm significantly exhibited anti-diabetic, anti-inflammation and protective effects against male and female infertility. Though the anticancer activity of date fruits was previously reported, the DPP anti-angiogenic effects were not reported, and as the first study, its inhibitory effects were examined in the current study. Methods: The DPP soft powder was collected to prepare its hydro-alcoholic extract to examine its anti-angiogenic activity in an in vitro model. At different concentrations, the cytotoxicity of the prepared extract was examined on human umbilical vein endothelial cells (HUVECs) using lactate dehydrogenase method. Cell proliferation was determined using the MTT assay and cytodex-3D model in collagen gel was used to assay its possible anti-angiogenic activity. The expression of VEGF, MMP-2 and MMP-9 genes was measured using real-time polymerase chain reaction (PCR). Finally, molecular docking simulation was used to highlight the possible role of DPP polyphenols to interact with the associated receptors. Results: The prepared hydro-alcoholic extract exhibited significant anti-angiogenic activity in a dose-dependent manner and decreased the endothelial cell proliferation. The calculated IC50 value for the examined extract in angiogenesis model was 260 µg·mL, respectively. Also, the expression of VEGF, MMP-2 and MMP-9 genes were significantly decreased. Docking simulation results unveiled that the isolated DPP polyphenols have the affinity to interact with ctDNA, VEGF and its receptors. Conclusion: The DPP is the new source of non-toxic anti-cancer agents to use as a dietary supplement in the pre-treatment of cancer.

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“…Moreover, Daoud et al, (2015) found that the pollen of Tunisian date palm cultivars contain high concentrations of flavonoids, and four types of flavonoids which include quercitin, rutin, catechin and epicatechin. Recently, AL-Samarrai et al, (2017) found that Iraqi pollen date palm contain many types of flavonoids (lincoceric acid, chlorogenic acid, ferulic acid, naringin, apigenin-7-O-beta glycopyranoside, letulin and letulin-7-O-beta glycosides).…”

Section: Table1 General Pollen Features Of the Examined Phoenix Dactylifera Cultivarsmentioning

confidence: 99%