Identification of Fertility Gene Rf1 in Collection Samples of Sorghum bicolor (L.) Moench in Southern Russia

Vozhzhova, N. N.; Ионова, Е. В.; Popov, A. M.; Kovtunov, V. V.

doi:10.3390/iecps2020-08710

Cited by 3 publications

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References 4 publications

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“…Опубликованы SSR-маркеры, наиболее близкие к локусу Rf1. Один из них -микросателлитный маркер Xtxp18использован для молекулярной идентификации аллелей гена Rf1 в коллекционных образцах Аграрного научного центра «Донской» (Vozhzhova et al, 2021). Наиболее вероятный ген-кандидат в локусе Rf2 -гомолог гена Rf1 риса Oryza sativa L, восстановитель фертильности пыльцы при ЦМС BТII-типа (Jordan et al, 2010).…”

Section: Introductionunclassified

Development and validation of CAPS-marker associated with the Rf2 gene in sorghum (Sorghum bicolor (L.) Moench)

Radchenko

Alpatieva

Karabitsina

et al. 2021

Biotehnologiâ i selekciâ rastenij

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Background. The development of heterotic hybrids based on cytoplasmic male sterility (CMS) is the leading strategy in breeding sorghum (Sorghum bicolor (L.) Moench). The trait of pollen fertility restoration in forms with CMS A1 (milo), predominantly used in sorghum breeding, is determined by at least two dominant complementary genes Rf1 and Rf2, and also gene Rf5. The development of accessible molecular markers of sorghum Rf genes is highly relevant for hybrid breeding, since they can significantly accelerate the process of creating female sterile forms (A lines), sterility maintainers (B lines) and pollen fertility restorers (R lines).Material and methods. The studied material included 36 sorghum accessions from the VIR collection, which differed by the ability to restore pollen fertility in forms with A1-type CMS. The nucleotide polymorphism of 935 bp fragments of the PPR genes Sobic.002G057050, Sobic.002G054100, and Sobic.002G054200 located at the chromosome 2 was studied.Results. The fragments obtained with the use of a pair of 2459403fw and 2459403 primers were 935 bp long and included parts of three genes: Sobic.002G057050, Sobic.002G054100, Sobic.002G054200. For identifying the sequence variant Sobic.002G057050-1090 associated with the Rf2 gene, Tru9 I restrictase was chosen, which allows obtaining a 572 bp fragment unique for all the studied R lines. Such a marker was found in 10 sorghum lines from West China and Kyrgyzstan, which are widely used in breeding as fertility restorers. The fragment was found neither in three lines with sterile cytoplasm and their fertile analogues, nor in 7 accessions of kafir sorghum, which lacked functional alleles of Rf genes.Conclusions. It has been demonstrated that the marker can be used for selection and checking purity of R and B/A lines. It is also applicable for verifying hybridity of F1 seeds and analyzing hybrid populations from crosses of R lines 924-4, 928-1, 929-3, 931-1, 933-1/6, 1237-3, 1243-2, 1251, 1150-1, F10BC2 with A lines Nizkorosloe 81s, А-83 and А-10598. It may be suggested that the ability to restore pollen fertility in R lines, which lack the marker CAPS- 572, is determined by another Rf gene. The studied 935 bp fragment of Sobic.002G057050 harbours 22 SNP, therefore the development of CAPS-markers for their identification and differentiation can be promising.

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Section: Introductionunclassified

Development and validation of CAPS-marker associated with the Rf2 gene in sorghum (Sorghum bicolor (L.) Moench)

Radchenko

Alpatieva

Karabitsina

et al. 2021

Biotehnologiâ i selekciâ rastenij

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Productive and nutritional potential of Sudan grass

Kovtunova,

Shishova

2023

Аграрная наука Евро-Северо-Востока

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Sudan grass is characterized by drought and heat resistance and is the best among annual and perennial bluegrass feed crops by the content of the main nutrient element - protein. The aim of the research is to evaluate 200 accessions and varieties of Sudan grass according to productivity and quality of green mass, to identify the sources of valuable traits and their correlation. The study was carried out in the Rostov region on ordinary carbonate black earth (chernozem). The collection of Sudan grass was evaluated in 2016-2019, of which contrasting weather conditions were characterized by 2017 (HTC = 0.85) and 2018 (HTC = 0.34). There were identified varieties and accessions that exceeded the standard variety Alexandrina according to green mass yield by 17.0-56.3 % (K-311, V-51/2, Chernoplenchataya 10, Alisa, K-187, Gratsiya, etc.); in terms of dry matter content – above the standard by 4.0-8.3 % (K-141/1, K-348/2, Svetloplenchataya 1, ‘Chernosemyannaya 191) and dry matter yield – by 26.4-60.5 % (Chernoplenchataya 10, Alisa, K-187, etc.). The sources of high content of crude protein include samples of the collection K-237, K-349/1, Topolek, K-311, K-161ch, Sudanka 24, Rossiyanka (14.82-16.13%); fiber - K-176/2, Ozornitsa, K-265/2, K-10257, etc. (38.8-40.4 %). The identified samples have been recommended for use in hybridization to develop new highly productive varieties and hybrids with high quality green mass. As a result of correlation-regression analysis, a weak negative relationship was established between the yield of green mass of Sudan grass and the content of crude protein in it, with an increase in protein content by 1 %, the yield decreases by 93.8 g/m2.

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Use of hybridization to develop new sorghum varieties and hybrids

Kovtunova,

Kovtunov,

Romanyukin

et al. 2024

Zernovoe hozâjstvo Rossii

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Hybridization is the main method for developing new breeding material, obtaining genetic variability, and combining desired traits. The purpose of the current work is to characterize new promising varieties and hybrids of sweet and grass sorghum developed by the “ARC “Donskoy” because of hybridization on a sterile and fertile basis. The study was conducted on the breeding field of the FSBSI “ARC “Donskoy”, Zernograd, Rostov region in 2021–2023. The zone is characterized by ordinary carbonate chernozem (3.2 % of humus, 10–40 mg/kg of phosphorus, 70–110 mg/kg of nitrogen, 300–500 mg per kg of soil of potassium). Meteorological conditions during the years of study (2021–2023) were contrasting: in 2022, HTC = 0.52 indicated average aridity and insufficient moisture with 0.81–0.82 in 2021 and 2023. The objects of the study were the varieties and hybrids of Sudan grass and sugar sorghum developed by the FSBSI “ARC “Donskoy”. As a result of hybridization on a sterile basis, there was developed a number of sorghumSudan hybrids (‘Jetta x Kudesnitsa’, ‘APV-1115 x Topolina’, ‘APV-1115 x FP’, ‘Dobrynya’, ‘Gordey’) and sweet sorghum hybrids (‘APV-115 x Yuzhnoye’, ‘Jetta x Severanay Vishnya’, ‘Duet’), allowing to obtain green mass yields of 51–63 and 47–49 t/ha, which was higher than the standard by 5–17 t/ha (10.8–37.0 %) and 5–7 t/ha (11.9–16.7 %) respectively. Using intervarietal hybridization, almost all varieties developed by the FSBSI “ARC “Donskoy” have been created. Among them there are the Sudan grass varieties ‘Alisa’, ‘Gratsiya’, ‘Anastasiya’, ‘Kudesnitsa’, ‘FP’ and the sweet sorghum varieties ‘Yuzhnoe’, ‘Feniks’, ‘Orfey’, ‘SK-20/334’, ‘Severanay Vishnya’ with a green mass yield of 40–48 and 34–38 t/ha, which is higher than that of standard varieties by 3–11 t/ha (8.1–29.7 %) and 7–11 t/ha (25.9–40.7 %), respectively. Thus, hybridization is the main method for developing new breeding material and combining the desired traits in new samples.

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Identification of Fertility Gene Rf1 in Collection Samples of Sorghum bicolor (L.) Moench in Southern Russia

Cited by 3 publications

References 4 publications

Development and validation of CAPS-marker associated with the <i>Rf2</i> gene in sorghum (<i>Sorghum bicolor</i> (L.) Moench)

Development and validation of CAPS-marker associated with the <i>Rf2</i> gene in sorghum (<i>Sorghum bicolor</i> (L.) Moench)

Productive and nutritional potential of Sudan grass

Use of hybridization to develop new sorghum varieties and hybrids

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