Identification of extractable growth factors from small intestinal submucosa

Voytik‐Harbin, Sherry L.; Brightman, Andrew O.; Kraine, Meredith R.; Waisner, Beverly; Badylak, Stephen F.

doi:10.1002/(sici)1097-4644(19971215)67:4<478::aid-jcb6>3.0.co;2-p

Cited by 536 publications

(245 citation statements)

References 37 publications

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“…SIS is mainly composed of collagen type 1 and contains endogenous growth factors such as FGF and TGF-b and other chemoattractants. [17][18][19] Several studies have shown that SIS can provide a collagenous matrix to promote cell migration into the healing site to enhance revascularization and repair. [20][21][22][23][24] It is hypothesized that SIS enhance and promote the healing of the ACL.…”

Section: Introductionmentioning

confidence: 99%

Healing of the Goat Anterior Cruciate Ligament After a New Suture Repair Technique and Bioscaffold Treatment

Nguyen

Geel

Schulze

et al. 2013

Tissue Engineering Part A

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Primary suture repair of the anterior cruciate ligament (ACL) has been used clinically in an attempt to heal the ruptured ACL. The results, however, were not satisfactory, which in retrospect can be attributed to the used suturing technique and the suboptimal healing conditions. These constraining conditions can be improved by introducing a new suturing technique and by using small intestinal submucosa (SIS) as a bioscaffold. It is hypothesized that the suturing technique keep the torn ends together and that SIS enhance and promote the healing of the ACL. The goat was used as the study model. In the Suture group, the left ACL was transected and suture repaired with a new locking suture repair technique (n = 5) allowing approximation and fixation under tension. The Suture-SIS group underwent the same procedure with the addition of SIS (n = 5). The right ACL served as control. After 12 weeks of healing, anterior-posterior translation and in situ force of the healing ACL were measured, followed by the measurement of the cross-sectional area and structural stiffness. Routine histology was performed on tissue samples. Gross morphology showed that the healing ACL was continuous with collagenous tissue in both groups. The cross-sectional area of the Suture and the Suture-SIS group was 35% and 50% of the intact control, respectively. The anterior-posterior translations at different flexion angles were statistically not different between the Suture group and the Suture-SIS group. Only the in situ force at 30°in the Suture-SIS group was higher than in the Suture group. Tensile tests showed that the stiffness for the Suture group was not different from the Suture-SIS group (31.1 -8.1 N/mm vs. 41.9 -18.0 N/mm [p > 0.05]). Histology showed longitudinally aligned collagen fibers from origo to insertion. More fibroblasts were present in the healing tissue than in the control intact tissue. The study demonstrated the proof of concept of ACL repair in a goat model with a new suture technique and SIS. The mechanical outcome is not worse than previously reported for ACL reconstruction. In conclusion, the approach of using a new suture technique, with or without a bioscaffold to heal the ACL is promising.

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Section: Introductionmentioning

confidence: 99%

Healing of the Goat Anterior Cruciate Ligament After a New Suture Repair Technique and Bioscaffold Treatment

Nguyen

Geel

Schulze

et al. 2013

Tissue Engineering Part A

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“…29 As the SIS contains bioactive agents including growth factors and fibronectin, 24 such chemoattractants could be responsible for the observed increase in neo-tissue formation in the SIS-treated PT defects to enhance the healing process.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, SIS contains a variety of growth factors capable of attracting healing cells through chemotaxis. 24 Further, SIS allows cellular infiltration only through its abluminal side, and thus, can prevent adhesion formation on its luminal side. 25,26 When a single layer of SIS was applied to a gap injury of the rabbit medial collateral ligament (MCL), the healing ligament had better collagen fiber alignment with larger collagen fibril diameters.…”

Section: Introductionmentioning

confidence: 99%

“…Using the right knee of the New Zealand White rabbit as a model, one layer of SIS was placed between the PT defect and the fat pad, and a second layer was placed over the anterior surface of the defect. As SIS has been shown to contain growth factors which can cause chemotaxis and to possess a preferential alignment of collagen fibers which may provide contact guidance to healing tissues, [24][25][26][27] the experiment was designed to test the hypothesis that SIS can cause increased cell proliferation and matrix production as well as improved matrix organization and biomechanical properties of the neo-PT. Secondly, based on the previous literature showing the ability of SIS to prevent cellular infiltration on its luminal side, 25,26 the SIS can serve as a natural barrier in order to limit cellular and vascular infiltration from the fat pad, thus limiting adhesion formation.…”

Section: Introductionmentioning

confidence: 99%

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Use of a bioscaffold to improve healing of a patellar tendon defect after graft harvest for ACL reconstruction: A study in rabbits

Karaoğlu

Fisher

Woo

et al. 2007

Journal Orthopaedic Research

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Following harvest of a bone-patellar tendon-bone (BPTB) autograft, the central third of the patellar tendon (PT) does not heal well. The healing tissues also form adhesions to the fat pad and can cause abnormal patellofemoral joint motion. The hypotheses were that a bioscaffold could enhance patellar tendon healing through contact guidance and chemotaxis, and the scaffold could serve as a barrier to decrease adhesion formation between the neo-PT and infrapatellar fat pad. In 20 New Zealand White rabbits, a central-third PT defect was created. One strip of porcine small intestinal submucosa (SIS) was attached to both the anterior and posterior sides of the PT defect of the SIS-treated group (n ¼ 10). For comparison, a central defect was left nontreated (n ¼ 10). At 12 weeks, histomorphology was examined using Masson's trichrome staining. The cross-sectional area (CSA) was determined with a laser micrometer, and the central BPTB complexes were tested in uniaxial tension. SIS-treated samples showed a greater amount of healing tissue with denser and well-oriented collagen fibers and more spindle-shaped cells. There was no noticeable adhesion formation in the SIS-treated group. For the nontreated group, there were significantly more and diffuse adhesive formations. The SIS-treated group also had a 68% increase in neo-PT CSA, 98% higher stiffness, and 113% higher ultimate load than that in the nontreated group. SIS treatment increased the quantity of healing tissue, improved the histological appearance and biomechanical properties of the neo-PT, and prevented adhesion formation between the PT and fat pad. ß

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“…These characteristics make SIS attractive for tissue regeneration in an unseeded fashion. 14,15 This result was recognized by Lantz and colleagues, who first used SIS in animal studies as a vascular patch, where it demonstrated tissue-specific regeneration in both arteries and veins. 16,17 Kropp and colleagues subsequently demonstrated, in both small and large animal models, that SIS alone can promote bladder regeneration when used as an unseeded graft.…”

Section: Unseeded Technologymentioning

confidence: 99%

Tissue engineering in urology

Matoka

Cheng

2013

CUAJ

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Tissue engineering encompasses a multidisciplinary approach geared toward the development of biological substitutes designed to restore and maintain normal function in diseased or injured tissues. This article reviews the basic technology that is used to generate implantable tissue-engineered grafts in vitro that will exhibit characteristics in vivo consistent with the physiology and function of the equivalent healthy tissue. We also examine the current trends in tissue engineering designed to tailor scaffold construction, promote angiogenesis and identify an optimal seeded cell source. Finally, we describe several currently applied therapeutic modalities that use a tissue-engineered construct. While notable progress has clearly been demonstrated in this emerging field, these efforts have not yet translated into widespread clinical applicability. With continued development and innovation, there is optimism that the tremendous potential of this field will be realized.Can Urol Assoc J 2009;3(5):403-8 Résumé L'ingénierie tissulaire englobe une approche multidisciplinaire axée sur le développement de substituts biologiques en vue de rétablir et de maintenir la fonction normale de tissus lésés. L'article qui suit passe en revue la technologie fondamentale utilisée pour générer des greffons implantables produits par ingénierie in vitro et possédant des caractéristiques in vivo correspondant aux tissus sains équivalents sur les plans physiologique et fonctionnel. Nous examinons également les tendances actuelles en ingénierie tissulaire visant à adapter des échafaudages tissulaires, à promouvoir l'angiogenèse et à dégager une source optimale de cellules implantables. Enfin, nous décrivons plusieurs modalités thérapeu-tiques actuellement mises en application utilisant un échafaudage créé par ingénierie tissulaire. En dépit de progrès remarquables dans ce domaine en effervescence, les efforts déployés ne se sont pas encore traduits par une applicabilité clinique étendue. Des développements et des percées continus permettent d'être optimiste face au potentiel prodigieux de ce domaine.

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Identification of extractable growth factors from small intestinal submucosa

Cited by 536 publications

References 37 publications

Healing of the Goat Anterior Cruciate Ligament After a New Suture Repair Technique and Bioscaffold Treatment

Healing of the Goat Anterior Cruciate Ligament After a New Suture Repair Technique and Bioscaffold Treatment

Use of a bioscaffold to improve healing of a patellar tendon defect after graft harvest for ACL reconstruction: A study in rabbits

Tissue engineering in urology

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