Identification of European and Asian pears using EST-SSRs from Pyrus

Bassil, Nahla V.; Postman, Joseph D.

doi:10.1007/s10722-009-9474-7

Cited by 50 publications

(29 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The observed heterozygosity at the different loci ranged from 0.14 (TXY16) to 0.89 (TXY104) with a mean of 0.49, which was lower than 0.63 reported in genomic SSR marker analysis in Pyrus Bao et al, 2007), consistent with the expectation that genebased SSRs developed from transcribed regions are less polymorphic than genome-derived SSRs. However, this was similar to values of 0.44 for Malus EST-SSRs in European pears (Wünsch and Hormaza, 2007) and 0.48 for Pyrus EST-SSRs in identification of European and Asian pears (Bassil and Postman, 2010). Another important index of the level of polymorphism, PIC values, displayed high polymorphism at each locus, ranging from 0.26 to 0.91 with an average of 0.75.…”

Section: Assessment Of the Genetic Relationship Among 28 Pyrus Accesssupporting

confidence: 80%

Development of genic SSR markers from transcriptome sequencing of pear buds

Yue

Liu

Zong

et al. 2014

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

A total of 8375 genic simple sequence repeat (SSR) loci were discovered from a unigene set assembled from 116 282 transcriptomic unigenes in this study. Dinucleotide repeat motifs were the most common with a frequency of 65.11%, followed by trinucleotide (32.81%). A total of 4100 primer pairs were designed from the SSR loci. Of these, 343 primer pairs (repeat length ≥15 bp) were synthesized with an M13 tail and tested for stable amplification and polymorphism in four Pyrus accessions. After the preliminary test, 104 polymorphic genic SSR markers were developed; dinucleotide and trinucleotide repeats represented 97.11% (101) of these. Twenty-eight polymorphic genic SSR markers were selected randomly to further validate genetic diversity among 28 Pyrus accessions. These markers displayed a high level of polymorphism. The number of alleles at these SSR loci ranged from 2 to 17, with a mean of 9.43 alleles per locus, and the polymorphism information content (PIC) values ranged from 0.26 to 0.91. The UPGMA (unweighted pair-group method with arithmetic average) cluster analysis grouped the 28 Pyrus accessions into two groups: Oriental pears and Occidental pears, which are congruent to the traditional taxonomy, demonstrating their effectiveness in analyzing Pyrus phylogenetic relationships, enriching rare Pyrus EST-SSR resources, and confirming the potential value of a pear transcriptome database for the development of new SSR markers.

show abstract

Section: Assessment Of the Genetic Relationship Among 28 Pyrus Accesssupporting

confidence: 80%

Development of genic SSR markers from transcriptome sequencing of pear buds

Yue

Liu

Zong

et al. 2014

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

show abstract

“…A variety of PCR-based DNA markers, such as random-amplified polymorphic DNA, microsatellite or simple sequence repeat (SSR), inter-simple sequence repeat (ISSR), amplified fragment length polymorphism, and sequence-related amplified polymorphism, provide the opportunity for fine-scale genetic characterizations, and still generate a large amount of data in a short period of time. Therefore, they are frequently used for genetic diversity assessment (Maguire et al, 2002;Gillaspie et al, 2005), variety identification (Hurtado et al, 1999;Bassil and Postman, 2010), genetic map construction (Piquemal et al, 2005;Celyon et al, 2008), pedigree analysis (Dreisigacker et al, 2004), etc.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis of sweet kernel apricot in China based on SSR and ISSR markers

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Simple sequence repeat (SSR) and inter-simple sequence repeat (ISSR) markers were used to evaluate genetic diversity among 22 sweet kernel apricot accessions and 12 cultivars in China to provide information on how to improve the utilization of kernel apricot germplasms. The results showed that 10 pairs of SSR primers screened from 40 primer pairs amplified 43 allelic variants, all of which were polymorphic (100%), and 9 ISSR primers selected from 100 primers amplified 67 allelic variants with 50 polymorphic bands (74.63%). There was a relatively distant genetic relationship between the 34 samples, where their genetic similarity coefficient was between 0.62 and 0.99. The UPGMA dendrogram constructed using combined data of the two marker systems separated the genotypes into three main clusters.

show abstract

“…SSR markers have been isolated and successfully used for cultivar identification, genetic diversity and relationship analysis of Pyrus (Yamamoto et al 2001(Yamamoto et al , 2002a(Yamamoto et al , b, 2004Kimura et al 2002;Wünsch and Hormaza 2007;Bao et al 2007;Katayama et al 2007;Bassil and Postman 2010). By now, the study on Ussurian Pear was not enough, especially with wild accessions.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of cultivated and wild Ussurian Pear (Pyrus ussuriensis Maxim.) in China evaluated with M13-tailed SSR markers

Cao

Tian

Gao

et al. 2011

Genet Resour Crop Evol

View full text Add to dashboard Cite

Eight genomic SSR markers with a M13 tail attached were used to assess the genetic diversity of 72 Ussurian Pear accessions (Pyrus ussuriensis Maxim.) in China. The M13-tailed method was effective in discriminating all the 32 wild accessions. All the 40 Ussurian Pear cultivars could be successfully discriminated with the exception of 4 sets of synonymies or spots. A total of 108 alleles were obtained with an average of 13.5 per locus. The expected heterozygosity, observed heterozygosity, and power of discrimination were 0.78, 0.63, and 0.86 respectively.

show abstract

Identification of European and Asian pears using EST-SSRs from Pyrus

Cited by 50 publications

References 42 publications

Development of genic SSR markers from transcriptome sequencing of pear buds

Development of genic SSR markers from transcriptome sequencing of pear buds

Genetic diversity analysis of sweet kernel apricot in China based on SSR and ISSR markers

Genetic diversity of cultivated and wild Ussurian Pear (Pyrus ussuriensis Maxim.) in China evaluated with M13-tailed SSR markers

Contact Info

Product

Resources

About