Identification of Equine Lactadherin-derived Peptides That Inhibit Rotavirus Infection via Integrin Receptor Competition

Civra, Andrea; Giuffrida, Maria Gabriella; Donalisio, Manuela; Napolitano, Lorenzo; Takada, Yoshikazu; Coulson, Barbara S.; Conti, Amedeo; Lembo, David

doi:10.1074/jbc.m114.620500

Cited by 20 publications

(19 citation statements)

References 57 publications

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“…African green monkey kidney cells (Vero cells; ATCC CCL-81) were grown as monolayers in Eagle's minimal essential medium (Gibco/BRL) supplemented with 10% fetal bovine serum and 1% antibiotic-antimycotic solution. The HRoV strains Wa (ATCC VR-2018), HRV408 (ATCC VR-2273), and HRV248 (ATCC VR-2274) and the BRoV strain NCDV (ATCC VR-1290) were activated with 5 µg/mL porcine pancreatic trypsin type IX (Sigma-Aldrich Co.) for 30 min at 37°C and propagated in MA104 cells using DMEM containing 0.5 µg of trypsin per mL as described previously (Civra et al, 2015). Viral titers are expressed as focus-forming units (FFU) per milliliter.…”

Section: Cell Lines and Virusesmentioning

confidence: 99%

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Colostrum from cows immunized with a veterinary vaccine against bovine rotavirus displays enhanced in vitro anti-human rotavirus activity

Civra

Altomare

Francese

et al. 2019

Journal of Dairy Science

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Human rotaviruses represent a major cause of severe diarrheal disease in infants and young children. The limited impact of oral vaccines on global estimates of rotavirus mortality and the suboptimal use of oral rehydration justify the need for alternative prophylactic and therapeutic strategies, especially for immunocompromised hosts. The protective effects of colostrum-the first milk produced during the initial 24 to 48 h after parturition-are well documented in the literature. In particular, the ingestion of hyperimmune bovine colostrum has been proposed as an alternative preventive approach against human rotavirus gastroenteritis. Although the immunization of pregnant cows with human rotavirus boosts the release of specific immunoglobulin G in bovine colostrum, it raises regulatory and safety issues. In this study, we demonstrated that the conventional bovine rotavirus vaccine is sufficient to enhance the anti-human rotavirus protective efficacy of bovine colostrum, thus providing a conservative approach to produce hyperimmune bovine colostrum, making it exploitable as a functional food.

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Section: Cell Lines and Virusesmentioning

confidence: 99%

“…Rotavirus-cell binding assays were performed as described previously (Civra et al, 2015). Trypsin-activated RoV strains Wa, HRV248, HRV408, and NCDV were treated as described for the neutralization assays.…”

Section: Rotavirus-cell Binding Assaymentioning

confidence: 99%

Colostrum from cows immunized with a veterinary vaccine against bovine rotavirus displays enhanced in vitro anti-human rotavirus activity

Civra

Altomare

Francese

et al. 2019

Journal of Dairy Science

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“…It has been reported that bioactive peptides from proteins in foods have several functions [2][3][4][5][6][7][8]. Milk casein yields many peptides through hydrolysis with digestive and other enzymes.…”

Section: The Search For Peptides Derived From Casein With Anti-allergmentioning

confidence: 99%

“…It has been reported that polyphenolic compounds, such as epigallocatechin gallate, show anti-allergic effects, but there have been few reports regarding the anti-allergenic effect of peptides. It has been reported that bioactive peptides from proteins in foods have several functions, such as reduction in blood pressure [2][3][4], activation of immune cells [5,6], antiviral actions [7], and improvement of lipid metabolism [8]. Milk casein yields many peptides through hydrolysis with digestive and other enzymes.…”

Section: Introductionmentioning

confidence: 99%

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The Anti-Allergic Effects of the His-Ala-Gln Tripeptide and Constituent Amino Acids

Tanaka¹,

Okamoto²

2017

Amino Acid - New Insights and Roles in Plant and Animal

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The type-1 allergy, as typified by allergic rhinitis, pollen, and food allergies, is defined as a hypersensitivity reaction, and its frequency is increasing worldwide. There is a need to develop therapeutic agents that either prevent sensitization to allergens or suppress the allergic response after initiation. It has been reported that various peptides show antiallergic effects, but there have been few reports concerning peptides derived from food. Previously, we studied the anti-allergic effect of His-Ala-Gln (HAQ), which is present in CE90GMM, a peptide mixture derived from milk casein. In this chapter, special emphasis is placed on the anti-allergic effects of the HAQ peptide in vitro and in vivo, and the effect of peptide binding, peptide sequence, number of amino acids, and the electron density of the amino acids is investigated.

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Preventative and therapeutic potential of animal milk components against COVID‐19: A comprehensive review

Singh¹,

Hernandez-Rauda

Peña‐Rodas

2023

Food Science & Nutrition

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The global pandemic of COVID‐19 is considered one of the most catastrophic events on earth. During the pandemic, food ingredients may play crucial roles in preventing infectious diseases and sustaining people's general health and well‐being. Animal milk acts as a super food since it has the capacity to minimize the occurrence of viral infections due to inherent antiviral properties of its ingredients. SARS‐CoV‐2 virus infection can be prevented by immune‐enhancing and antiviral properties of caseins, α‐lactalbumin, β‐lactoglobulin, mucin, lactoferrin, lysozyme, lactoperoxidase, oligosaccharides, glycosaminoglycans, and glycerol monolaurate. Some of the milk proteins (i.e., lactoferrin) may work synergistically with antiviral medications (e.g., remdesivir), and enhance the effectiveness of treatment in this disease. Cytokine storm during COVID‐19 can be managed by casein hydrolyzates, lactoferrin, lysozyme, and lactoperoxidase. Thrombus formation can be prevented by casoplatelins as these can inhibit human platelet aggregation. Milk vitamins (i.e., A, D, E, and B complexes) and minerals (i.e., Ca, P, Mg, Zn, and Se) can have significantly positive effects on boosting the immunity and health status of individuals. In addition, certain vitamins and minerals can also act as antioxidants, anti‐inflammatory, and antivirals. Thus, the overall effect of milk might be a result of synergistic antiviral effects and host immunomodulator activities from multiple components. Due to multiple overlapping functions of milk ingredients, they can play vital and synergistic roles in prevention as well as supportive agents during principle therapy of COVID‐19.

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Identification of Equine Lactadherin-derived Peptides That Inhibit Rotavirus Infection via Integrin Receptor Competition

Cited by 20 publications

References 57 publications

Colostrum from cows immunized with a veterinary vaccine against bovine rotavirus displays enhanced in vitro anti-human rotavirus activity

Colostrum from cows immunized with a veterinary vaccine against bovine rotavirus displays enhanced in vitro anti-human rotavirus activity

The Anti-Allergic Effects of the His-Ala-Gln Tripeptide and Constituent Amino Acids

Preventative and therapeutic potential of animal milk components against COVID‐19: A comprehensive review

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