Identification of entomopathogenic bacteria associated with the invasive pest Drosophila suzukii in infested areas of Germany

Hiebert, Nils; Carrau, Tessa; Bartling, Merle T.; Vilcinskas, Andreas; Lee, Kwang-Zin

doi:10.1016/j.jip.2020.107389

Cited by 14 publications

(9 citation statements)

References 49 publications

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“…Since D. suzukii is responsible for significant yield reductions, a series of control strategies have been proposed to limit outbreaks and reduce the damages caused. Most of these are based on the use of agrochemicals such as wide-spectrum insecticides [6,15], but the increasingly restrictive laws in terms of pesticide usage is motivating researchers to investigate alternative control strategies, such as the use of natural enemies [16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Modelling Drosophila suzukii Adult Male Populations: A Physiologically Based Approach with Validation

Rossini

Contarini

Giarruzzo

et al. 2020

Insects

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The Spotted Wing Drosophila (SWD), Drosophila suzukii (Matsumura), is a harmful insect pest for soft fruit cultivations. Even though its main hosts belong to the genera Prunus and Rubus, its high polyphagy and adaptability to new environments makes it a serious problem for farmers worldwide, who have reported several economic losses because of this pest. A wide series of proposals to control SWD are available and operate in line with the mechanisms of integrated pest management, demonstrating their high efficiency when applied at the opportune moment. This work aims to apply and validate a physiologically based model which summarises all the available information about D. suzukii biology, such as the relationship between environmental temperature and its development, fertility and mortality rates. The model provided, as a result, a description of a population of SWD females taking into consideration the multiple generations that occurred during the year. Simulations were then compared with field data collected in a three-year survey in two experimental fields located in the Sabina Romana area (Lazio, Italy). More specifically, D. suzukii males were monitored with traps in fields cultivated with mixed varieties of cherries and they were selected because of their clearer identification in comparison to females. Results showed a high level of reliability of simulations in representing the field data, highlighting at the same time that there is no discrepancy in simulating D. suzukii females in order to represent male populations.

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Section: Introductionmentioning

confidence: 99%

Modelling Drosophila suzukii Adult Male Populations: A Physiologically Based Approach with Validation

Rossini

Contarini

Giarruzzo

et al. 2020

Insects

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“…Pathogens have been widely used as biological control agents for insects [11,[27][28][29], particularly the bacterium B. thuringiensis [64,65] and the fungus B. bassiana [11]. We previously showed that oral infection with the lactic acid bacterium L. pseudomesenteroides was able to kill the invasive fruit pest D. suzukii [54]. Here, we tested this pathogen against three target insects (two drosophilids and one aphid) via the oral and septic infection routes, in each case testing the effect of live bacteria at different concentrations and the effect of cell-free extracts representing different culture durations.…”

Section: Discussionmentioning

confidence: 99%

“…The L. pseudomesenteroides strain was isolated from moribund D. suzukii larvae as previously described [54]. Pre-cultures of L. pseudomesenteroides were prepared in 100-mL Erlenmeyer flasks containing 30 mL MRS medium (10 g/L casein peptone tryptic digest, 10 g/L meat extract, 5 g/L yeast extract, 20 g/L glucose, 1 g/L Tween-80, 2 g/L K 2 HPO 4 , 5 g/L sodium acetate, 2 g/L ammonium citrate, 0.2 g/L MgSO 4 × 7H 2 O, 0.05 g/L MnSO 4 × H 2 O).…”

Section: Cultivation Of L Pseudomesenteroides and Preparation Of Extmentioning

confidence: 99%

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The Gram-Positive Bacterium Leuconostoc pseudomesenteroides Shows Insecticidal Activity against Drosophilid and Aphid Pests

Hiebert

Kessel

Škaljac

et al. 2020

Insects

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Insect pests reduce global crop yields by up to 20%, but the most effective control measures are currently based on environmentally hazardous chemical pesticides. An alternative, ecologically beneficial pest-management strategy involves the use of microbial pathogens (or active compounds and extracts derived from them) that naturally target selected insect pests. A novel strain of the bacterium Leuconostoc pseudomesenteroides showed promising activity in our preliminary tests. Here, we investigated its effects in more detail, focusing on drosophilid and aphid pests by testing the survival of two species representing the family Drosophilidae (Drosophila suzukii and D. melanogaster) and one representing the family Aphididae (Acyrthosiphon pisum). We used oral and septic infection models to administer living bacteria or cell-free extracts to adult flies and aphid nymphs. We found that infection with living bacteria significantly reduced the survival of our insect models, whereas the administration of cell-free extracts had a significant effect only in aphids. These results confirm that L. pseudomesenteroides has potential as a new biocontrol agent for sustainable pest management.

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“…It is a rare food-borne opportunistic pathogen that has been isolated from fruits like cherries, grapes, and strawberries. Incidentally, T. ptyseos is known to be the dominant endosymbionts in the microbiome of a fruit fly known as Drosophila suzukii because it feeds exclusively on fresh fruits like cherries, grapes, and strawberries, resulting in T. ptyseos being the dominant endosymbiont in its microbiome unlike the case with other fruit flies like Drosophila melanogaster that preferentially feed on decaying fruits (Hiebert et al, 2020;Chandler et al, 2014). The feeding habit of D. suzukii has been determined to be influenced by attraction to a volatile organic compound (Bueno et al, 2019) known as 2,5diketo-D -gluconic acid that is produced by T. ptyseos (Tracz et al, 2015;Kageyama et al, 1992) and is also responsible for the spread and contamination of other fruits with T. ptyseos.…”

Section: Signmentioning

confidence: 99%

Health risk associated with eating fish from brackish water

Ndeta¹,

Lamsis²,

Portales³

et al. 2021

J. Med. Lab. Diagn.

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Heart disease is a leading cause of morbidity and mortality in the United States with diet being a major contributor. As solution, fish rich in omega-3 fatty acids is recommended. In this study, the impact of brackish water on fish quality, was done by sampling canal water in Edinburg, Texas. Canal water was membrane filtered and cultured on bacteriological media. Pure cultures were phenotypically identified, and isolates confirmed on a BD Phoenix analyzer. The BD Phoenix analyzer, confirmed the following isolates: Grimontia hollisae, Pseudomonas oryzihabitans, Pseudomonas pseudoalcaligenes, Tatumella ptyseos, Pseudomonas fluorescens, Klebsiella pneumonia subsp. ozaenae, Staphylococcus pettenkoferi, Pasteurella pneumotropica, Achromobacter species, Pasteurella aerogenes, Moraxella species and Pseudomonas species. Fish consumption is good for maintaining a healthy heart, but consumers risk exposure to foodborne pathogens that can cause watery diarrhea, severe abdominal cramps, nausea, vomiting, fever, bacteremia, urinary tract infections and even dead.

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Identification of entomopathogenic bacteria associated with the invasive pest Drosophila suzukii in infested areas of Germany

Cited by 14 publications

References 49 publications

Modelling Drosophila suzukii Adult Male Populations: A Physiologically Based Approach with Validation

Modelling Drosophila suzukii Adult Male Populations: A Physiologically Based Approach with Validation

The Gram-Positive Bacterium Leuconostoc pseudomesenteroides Shows Insecticidal Activity against Drosophilid and Aphid Pests

Health risk associated with eating fish from brackish water

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