Identification of Enterococcus faecalis in Root-filled Teeth With or Without Periradicular Lesions by Culture-dependent and—Independent Approaches

Zoletti, Gustavo O.; Siqueira, José F.; Santos, Kátia Regina Netto dos

doi:10.1016/j.joen.2006.02.001

Cited by 123 publications

(104 citation statements)

References 32 publications

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“…A seleção da cepa bacteriana desta pesquisa baseou-se na observação de que, na maioria das vezes, o fracasso no tratamento endodôntico está relacionado à presença de E. faecalis 44,45 . Tabela 1.…”

Section: Discussionunclassified

Estudo da ação antibacteriana do extrato hidroalcoólico de própolis vermelha sobre Enterococcus faecalis

Siqueira

Dantas

Gomes

et al. 2014

Rev. odontol. UNESP

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ResumoIntrodução: A própolis é uma substância resinosa e complexa; produzida pelas abelhas, destaca-se por suas propriedades terapêuticas, como atividade antimicrobiana, anti-inflamatória e cicatrizante. Poucos trabalhos existem sobre a variedade de própolis vermelha, encontrada no Estado de Sergipe. Objetivo: Avaliar a ação antimicrobiana do extrato de própolis vermelha, coletada na região nordeste do Estado de Sergipe, contra cepas de Enterococcus faecalis. Material e método: As amostras de própolis vermelha foram coletadas em Brejo Grande-SE, Brasil, e identificadas segundo suas características sensoriais, a granulometria e requisitos físico-químicos. O teor de flavonoides no extrato seco foi determinado. Soluções de própolis vermelha (EEP) foram preparadas nas concentrações de 1%; 2,5%; 5% e 7,5%. A cepa bacteriana de referência utilizada foi Enterococcus faecalis -ATCC 29212. A atividade antibacteriana foi verificada por meio de testes in vitro (teste de difusão em disco e determinação da concentração bactericida mínima -CBM) e ex vivo (utilizando dentes humanos extraídos). No teste ex vivo, os dentes contaminados foram divididos em três grupos com dez dentes cada. O grupo 1 foi tratado com própolis a 7,5% (concentração determinada no teste CBM); o grupo 2 foi tratado como controle positivo, com solução de hipoclorito de sódio a 2,5%, e o grupo 3 foi utilizado como controle negativo, sendo tratado apenas com solução salina NaCl 0,9%. Resultado: O extrato de própolis promoveu halo de inibição comparado ao da solução de hipoclorito de sódio a 2,5%, variando entre 12 e 16 mm. Não houve crescimento bacteriano após irrigação do conduto radicular com a solução de EEP a 7,5%. Conclusão: A própolis coletada apresentou médio teor de flavonoides (1,8%) e características físico-químicas coerentes com as exigidas pelo Ministério da Agricultura. Na concentração de 7,5% de própolis vermelha, foi observado um maior potencial antibacteriano quando comparado aos demais grupos. Descritores: Própolis; bactérias; Enterococcus faecalis. AbstractIntroduction: Propolis is a complex resinous substance produced by bees that has therapeutic properties, such as antimicrobial activity, anti-inflammatory, healing. Few studies exist on the red variety of propolis, found in the state of Sergipe. Objective: Evaluation of the antimicrobial action of the extract of propolis red collected in the northeastern state of Sergipe, against strains of Enterococcus faecalis. Material and method: The red propolis samples were collected in Brejo Grande/SE -Brazil and identified according to their sensory characteristics, granulometry and physical chemical requirements. The content of flavonoids in dried extract was determined. Solutions of red propolis (EEP) were prepared at concentrations of 1%; 2.5%; 5% and 7.5%. The bacterial strain used was Enterococcus faecalis -ATCC 29212. The antibacterial activity was verified by in vitro tests (disk diffusion test and determination of minimum bactericidal concentration -CBM) and ex vivo (using human extracted...

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Section: Discussionunclassified

Estudo da ação antibacteriana do extrato hidroalcoólico de própolis vermelha sobre Enterococcus faecalis

Siqueira

Dantas

Gomes

et al. 2014

Rev. odontol. UNESP

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“…The content from the root canal was absorbed with 4 sterile paper points. Each paper point was maintained in position inside the duct at the established working length for one minute and then transferred into a tube with 1 mL TSB 15 . From this sample, an aliquot was taken to perform bacterial counts and the remainder was incubated for 24-48 h. If the plate count was negative, the remainder of the sample was allowed to seed on a KF Streptococcus agar plate (Merck, D-61 Darmstadt, Germany) to definitively rule out the presence of Enterococcus spp.…”

Section: Sampling Proceduresmentioning

confidence: 99%

“…DNA bands were visualized with a UV White Light transilluminator (Vilber Lourmat, Torcy, Z.I. Sud, France) 15,18 . Later, gels were photographed with a Kodak ID LPU 120 camera (Rochester, NY, USA) and analysed by the ID 3.02 Kodak Digital Science Software (Rochester, NY, USA).…”

Section: Molecular Identification By Pcrmentioning

confidence: 99%

Enterococcus spp. isolated from root canals with persistent chronic apical periodontitis in a Chilean population

Sánchez‐Sanhueza¹,

González-Rocha²,

Dominguez³

et al. 2015

Braz. J. Oral Sci.

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Aim: To isolate and identify in a Chilean population, Enterococcus spp. from root canals with persistent chronic apical periodontitis (CAP) and to investigate the potential correlation between the bacteria and the observed clinical features. Methods: Twenty patients with indication for endodontic retreatment due to persistent CAP were selected. Data from patient general health and dental clinical history were recorded. During retreatment, a microbial sample was obtained from the root canal and inoculated in a selective Enterococcus medium. Using bacterial cultivation methods, bacterial isolates belonging to the genus Enterococcus were identified. The relationship between the number of colony-forming units of Enterococcus spp. and patient clinical data was assessed statistically by the Pearson Chi square and Fisher exact tests. Finally, a Polymerase Chain Reaction (PCR) assay to determine the most prevalent species of Enterococcus spp. was conducted in the clinical samples, and the results were analyzed by a proportion comparison test. Results: Enterococcus spp. strains were isolated in 70% of the patients. Most of them (98.8%) accounted for Enterococcus faecalis and only 1.2% for Enterococcus faecium. A high frequency of E. faecalis was found in teeth with inadequate endodontic treatment or dental crown restorations. Conclusions: This study concluded that E. faecalis is prevalent in root canals with persistent CAP in a Chilean population. E. faecium as found in a single case with the poorest root canal filling. Further studies are still required to investigate the presence of other species, which may be linked to persistent chronic apical periodontitis.

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“…Microbiological culturing techniques and molecular biological methods have been employed to investigate the microbiota from post-treatment root canal infections and have demonstrated that Enterococcus faecalis (E. faecalis) was highly prevalent in teeth that had undergone unsuccessful root canal therapies (3,4). E. faecalis possesses numerous virulence factors, including cytolysin, lytic enzymes, pheromones, aggregation substances and lipoteichoic acid, and transmits these virulence traits into other species, further contributing to apical periodontitis (5,6).…”

Section: Introductionmentioning

confidence: 99%

Relevance of the clustered regularly interspaced short palindromic repeats of Enterococcus faecalis strains isolated from retreatment root canals on periapical lesions, resistance to irrigants and biofilms

Tong

Ling

et al. 2017

Exp Ther Med

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Abstract.A high prevalence of Enterococcus faecalis (E. faecalis) is observed in teeth with root canal treatment failures. Clustered regularly interspaced short palindromic repeats (CRISPR) are widely distributed in prokaryotes that have adaptive immune systems against mobile elements, including pathogenic genes. The present study investigated the relevance of the CRISPR in E. faecalis strains isolated from retreated root canals on biofilms, periapical lesions and drug resistance. A total of 20 E. faecalis strains were extracted from the root canals of teeth referred for root canal retreatment. CRISPR-Cas loci were identified by two pairs of relevant primers and polymerase chain reaction. The susceptibility of the 20 isolated strains to intracanal irrigants was evaluated by 1-and 5-minute challenges with a mixture of a tetracycline isomer, an acid and a detergent (MTAD), 2% chlorhexidine (CHX) and 5.25% sodium hypochlorite (NaOCl). The microtiter plate assay and crystal violet staining were used to compare the biofilm formation of the E. faecalis isolate strains. Out of the 20 E. faecalis isolate strains, 5 strains that lacked CRISPR-cas determinants exhibited significant periapical lesions. Among the 15 strains containing CRISPR-cas determinants, 8 were isolated from root canals with inadequate fillings and 7 were isolated from root canals without any fillings. The five strains lacking CRISPR-cas loci were observed to be more resistant to MTAD and 2% CHX than the 15 strains that had CRISPR-cas loci. All of the strains exhibited the same susceptibility to 5.25% NaOCl. Furthermore, the 5 strains lacking CRISPR-cas determinants generated more biofilm than the other 15 strains. Thus, the results of the present study suggested that E. faecalis root canal isolates lacking CRISPR-cas exhibit higher resistance to intracanal irrigants, stronger biofilm formation and generate significant periapical lesions. IntroductionPost-treatment apical periodontitis is typically caused by diverse pathogens, including Gram-positive and Gram-negative bacteria, and is characterized by the appearance and persistence of apical periodontic lesions (1,2). Microbiological culturing techniques and molecular biological methods have been employed to investigate the microbiota from post-treatment root canal infections and have demonstrated that Enterococcus faecalis (E. faecalis) was highly prevalent in teeth that had undergone unsuccessful root canal therapies (3,4). E. faecalis possesses numerous virulence factors, including cytolysin, lytic enzymes, pheromones, aggregation substances and lipoteichoic acid, and transmits these virulence traits into other species, further contributing to apical periodontitis (5,6). Furthermore, E. faecalis may overcome the challenges of starvation and intracanal medication and persist as a pathogenic bacterium in the root canal system (7). When an adequate nutrition supply becomes available, the starved E. faecalis recover, resulting in increased pathogenicity (8).Therefore, E. faecalis may serve an important ...

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Identification of Enterococcus faecalis in Root-filled Teeth With or Without Periradicular Lesions by Culture-dependent and—Independent Approaches

Cited by 123 publications

References 32 publications

Estudo da ação antibacteriana do extrato hidroalcoólico de própolis vermelha sobre Enterococcus faecalis

Estudo da ação antibacteriana do extrato hidroalcoólico de própolis vermelha sobre Enterococcus faecalis

Enterococcus spp. isolated from root canals with persistent chronic apical periodontitis in a Chilean population

Relevance of the clustered regularly interspaced short palindromic repeats of Enterococcus faecalis strains isolated from retreatment root canals on periapical lesions, resistance to irrigants and biofilms

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