Identification of enteric Helicobacter in avian species

Nebbia, Patrizia; Tramuta, Clara; Ortoffi, Marco Francesco; Bert, E. Sunyol I; Sola, S. Cerruti; Robino, P.

doi:10.1024/0036-7281.149.9.403

Cited by 31 publications

(21 citation statements)

References 18 publications

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“…pullorum may infect the intestinal tracts of avian species and humans. It has been detected in the intestinal contents of broiler chickens, laying hens (2,9,20,28,32), and guinea fowls (20) and in a psittacine bird (10). Moreover, H. pullorum has been isolated in fecal samples from humans with gastroenteritis (5,8,18,19,29) and its DNA was isolated from the biliary trees and gallbladders of women suffering from chronic cholecystitis and from livers of patients suffering from cirrhosis and/or hepatocellular carcinoma (12,22,24,26).…”

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“…In Italy, Zanoni et al (32) tested the cecal contents of 60 animals from nine commercial laying hen and six broiler chicken farms and found that 51 broiler chickens were H. pullorum positive by using the modified filter technique of Steel and McDermott (28) and incubating the plates before removing the filters under a hydrogen atmosphere. Nebbia and colleagues (20) found 80% of 130 DNA poultry samples (i.e., chickens, laying hens, and Guinea fowl) were H. pullorum positive by 16S rRNA gene sequence analysis.…”

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Prevalence ofHelicobacterpullorumin Conventional, Organic, and Free-Range Broilers and Typing of Isolates

Manfreda

Parisi²,

Lucchi

et al. 2011

Appl Environ Microbiol

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Helicobacter pullorum represents a potential food-borne pathogen, and avian species appear to be a relevant reservoir of this organism. In this study, the prevalence of H. pullorum was investigated at 30 conventional farms where 169 ceca from 34 flocks were tested, at eight organic farms where 39 ceca from eight flocks were tested, and at seven free-range farms where 40 ceca from eight flocks were tested. All of the ceca were obtained from healthy broiler chickens. Moreover, amplified fragment length polymorphism, pulsed-field gel electrophoresis, and automated ribotyping were employed to estimate the levels of genetic variability of H. pullorum broiler isolates within and between flocks. Overall, Gram-negative, slender, curved rods, identified as H. pullorum by PCR, were isolated at 93.3% of the farms tested. The percentage of positive free-range farms (54.2%) was significantly lower than that of conventional (100%) or organic (100%) farms (P < 0.001). The level of within-flock genetic variability, calculated as the number of flocks colonized by isolates genetically different by all of the typing methods, was 34.9%. Isolates showing identical profiles by each typing method were observed in 11.6% of the flocks, but they were never detected between flocks. However, groups of isolates clustered together with an overall similarity level of >85%. Our results suggest that even though a high level of genetic variability is attributable to H. pullorum broiler isolates, their hierarchical genotyping produces data useful for epidemiological investigations.

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Prevalence ofHelicobacterpullorumin Conventional, Organic, and Free-Range Broilers and Typing of Isolates

Manfreda

Parisi²,

Lucchi

et al. 2011

Appl Environ Microbiol

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“…Previous reports showed that H. Pullorum could infect the digestive system of human and avian species. This bacterium was isolated from digestive contents of broiler chicken, laying hens [1,7] , guinea fowl [8] and in a psittacin bird [5] . Moreover, H. pullorum has been isolated in fecal samples from humans with gastroenteritis in the UK, Canada, Germany and Switzerland [9] and its DNA was detected from gallbladder of a woman suffering from chronic cholecystitis and from livers of patients suffering from cirrhosis and/or hepatocarcinoma [10,11] .…”

Section: Discussionmentioning

confidence: 99%

Evcil Kuşların Sekum İçeriklerinden Helicobacter pullorum 16S rRNA Geninin Amplifikasyonu

Gholami‐Ahangaran¹,

Azargoon²,

Anari³

2015

Kafkas Univ Vet Fak Derg

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For evaluation of pet birds as a resource of Helicobacter pullorum, 100 cecal content samples were collected from 50 finches and 50 Australian parrots, with no clinical signs suggestive of systemic and/or enteric disease, in Iran. DNA was extracted from cecal samples and PCR was carry out for identification of H. pullorum 16S rRNA gene. Results showed, H. pullorum DNA was detected in 3 of the 50 (6%) cecal samples of finch and 6 out of 50 (12%) cecal samples of Australian parrot. This study represents that finch and Australian parrot can be a source of H. pullorum infection and serve as reservoirs for the H. pullorum. Keywords: Australian Parrot, Finch, Helicobacter pullorum Evcil Kuşların Sekum İçeriklerinden Helicobacter pullorum 16S rRNA Geninin Amplifikasyonu ÖzetHelicobacter pullorum kaynağı olarak evcil kuşların değerlendirilmesi amacıyla İran'da sistemik veya enterit klinik belirtileri göstermeyen 50 ispinoz ve 50 Avustralya papağanından toplam 100 sekum içeriği örneği toplandı. Sekal örneklerden DNA ekstraksiyonu yapıldı ve H. pullorum 16S rRNA geninin identifikasyonu amacıyla PCR gerçekleştirildi. H. pullorum geni 50 ispinozun 3'ünde (%6) ve 50 Avustralya papağanının 6'sında (%12) belirlendi. Çalışmanın sonuçları ispinoz ve Avustralya papağanının H. pullorum enfeksiyonu kaynağı ve bu etken için reservuar olabileceğini göstermiştir.

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“…The organism is suspected to cause vibrionic hepatitis in chickens. Infection with this organism is most often associated with farmraised birds, including chickens, turkeys and guinea fowl (Nebbia et al, 2007;Stanley et al, 1994). In one report, H. pullorum was isolated from human faeces 3 months following the patients' initial presentation with diarrhoea (Steinbrueckner et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Natural and experimental Helicobacter pullorum infection in Brown Norway rats

Cacioppo

Turk

Shen

et al. 2012

Journal of Medical Microbiology

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Helicobacter pullorum is an enterohepatic Helicobacter species (EHS) that was recently reported as a naturally acquired infection in mice. Faecal samples from 18 out of 20 Brown Norway (BN) rats, housed in the same barrier as the H. pullorum-infected mice, were positive for H. pullorum using species-specific PCR. In addition, we determined whether H. pullorum was able to persistently colonize the gastrointestinal tract and/or biliary tree and elicit tissue inflammation as well as a serum IgG response in BN rats. Six (four male, two female) 6-week-old, H. pullorumnegative BN rats were orally dosed with 4¾10 8 c.f.u. of H. pullorum every other day for a total of three doses. At 2 weeks post-infection, all rats were H. pullorum-positive by faecal PCR. Five out of the six BN rats remained H. pullorum-positive for the entire 30 week study. PCR analysis of tissue collected at necropsy confirmed that the colon and caecum were the primary sites of H. pullorum colonization. Rats that were persistently colonized by H. pullorum had a sustained H. pullorum-specific IgG response measured by ELISA. Intestinal or hepatic pathology associated with H. pullorum infection was not noted. To our knowledge, this is the first report documenting that rats can be persistently colonized with an EHS that also infects humans.

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Identification of enteric Helicobacter in avian species

Cited by 31 publications

References 18 publications

Prevalence ofHelicobacterpullorumin Conventional, Organic, and Free-Range Broilers and Typing of Isolates

Prevalence ofHelicobacterpullorumin Conventional, Organic, and Free-Range Broilers and Typing of Isolates

Evcil Kuşların Sekum İçeriklerinden Helicobacter pullorum 16S rRNA Geninin Amplifikasyonu

Natural and experimental Helicobacter pullorum infection in Brown Norway rats

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