Identification of endophytic fungi from leaves of Pandanaceae based on their morphotypes and DNA sequence data from southern Thailand

Abstract

The authors established the taxonomic status of endophytic fungi associated with leaves of Pandanaceae collected from southern Thailand. Morphotypes were initially identified based on their characteristics in culture and species level identification was done based on both morphological characteristics and phylogenetic analyses of DNA sequence data. Twenty-two isolates from healthy leaves were categorised into eight morphotypes. Appropriate universal primers were used to amplify specific gene regions and phylogenetic analyses were performed to identify these endophytes and established relationships with extant fungi. The authors identified both ascomycete and basidiomycete species, including one new genus, seven new species and nine known species. Morphological descriptions, colour plates and phylogenies are given for each taxon. RESEARCH ARTICLESaowaluck Tibpromma et al. / MycoKeys 33: 25-67 (2018) 26

Re-evaluation of Mycoleptodiscus species and morphologically similar fungi

“…Notes — Mycoleptodiscus endophyticus was introduced based on molecular data of LSU, SSU, and tef1 sequences (Tibpromma et al 2018). Our phylogenetic analysis demonstrates that this taxon does not belong to Mycoleptodiscus s.str.…”

“…Subsequently, Sutton & Alcorn (1990) emended the generic concept to include species with 0–2-septate conidia with polar and lateral appendages or lacking appendages, and frequently producing appressoria (Sutton & Hodges 1976, Sutton & Alcorn 1990, Alcorn 1994, Whitton et al 2012). Currently, Mycoleptodiscus comprises 18 species (Ostazeski 1967, Sutton & Hodges 1976, Sutton & Alcorn 1985, 1990, Matsushima 1987, 1993, Bills & Polishook 1992a, Alcorn 1994, Ando 1996, Whitton et al 2012, Tibpromma et al 2018). The sexual morph has not been described for any species of the genus, except for M. affinis , which was introduced as Omnidemptus affinis by Cannon & Alcorn (1994).…”

“…Although Mycoleptodiscus species have been widely reported as plant pathogens, some have been isolated as endophytes from Chamaecyparis thyoides (Bills & Polishook 1992a, b), the aquatic Myriophyllum spicatum in the USA (Shearer 2001), Desmotes incomparabilis in Panama (Martínez-Luis et al 2011, Ortega et al 2013), Acer truncatum and Cinnamomum camphora in China (Sun et al 2011, He et al 2012), Borreria verticillata and Opuntia ficus-indica in Brazil (Andrioli et al 2012, Bezerra et al 2012), and Freycinetia sp. in Thailand (Tibpromma et al 2018). Mycoleptodiscus terrestris has also been isolated as an endophyte from aquatic submersed macrophytes in New Zealand (Hofstra et al 2009, 2012).…”

Análisis Filogenético de Secuencias ITS Provenientes de Hongos Endófitos Utilizando Inferencia Bayesiana Paralela de Árboles con Exabayes

“…Fungi and particularly endophytes are a very promising source of novel biologically active compounds [3] so studying them may help to implement new techniques for new biotechnological applications. An essential part of ecological studies of endophytic fungi is the taxonomic classification and phylogenetic inference [4], [5]. Fungi taxonomy is certainly complex, even now, what was believed to be one species can be in fact be an assemblage of productively isolated lineages.…”

“…ITS region is accepted as a barcode for fungi because of the higher amplification success rate for many fungal groups [7] [8]. Thanks to the next generation sequences the amount of data obtained in the ecological studies has increased exponentially through the years [9], [5], which in turn implies a challenge for the processing and analysis of the information.…”