Identification of endogenous outward currents in the human embryonic kidney (HEK 293) cell line

The maxi-anion channel: a classical channel playing novel roles through an unidentified molecular entity

“…These channels were similar to those observed in cultured cortical astrocytes from rats [4,49,50] and mice [5][6][7]51] as well as in a rat astrocytic RGCN cell line [52]. In epithelia, the urinary bladder [53], gastric [54], pancreatic [55][56][57], colonic [58][59][60], airway [61][62][63], choroid plexus [64], bile duct [65,66], ciliary [67][68][69], renal [9,19,[70][71][72][73][74][75][76][77][78], vestibular [79], placental [80][81][82][83][84][85][86], ruminal [87] and ovarian [84] epithelial cells were also found to express the maxianion channel with properties similar to those of excitable cells. Resembling maxi-anion channels were also found in fibroblasts [14,…”

Kir2.3 isoform confers pH sensitivity to heteromeric Kir2.1/Kir2.3 channels in HEK293 cells

“…Nevertheless, our major objective, i.e., to demonstrate that the presence of one or more subunits of the Kir2.3 isoform in the heterotetrameric channels will modify the channel sensitivity to protons, was achieved. Finally, endogenous outward currents have been reported in HEK293 cells [30][31][32]. We found rare occurrences of unitary events with conductances smaller than 4 pS during single channel recordings.…”

Voltage-dependent Changes of TRPV6-mediated Ca2+ Currents

“…1A, top). The I-V relationship showed a negative reversal potential, most likely caused by a basal Cl Ϫ conductance present in these cells (15). The inward current at potentials around 30 mV may arise through the activation of voltage-dependent channels, which are expressed in this cell line (16) and are not deactivated at such negative potentials (Fig.…”