Identification of effective combinatorial markers for quality standardization of herbal medicines

Shi, Zi-Qi; Song, De-Fang; Li, Ruo-Qian; Yang, Hua; Qi, Lian‐Wen; Xin, Gui-Zhong; Wang, Ding-Qian; Song, Hui‐Peng; Chen, Jun; Hao, Haiping; Li, Ping

doi:10.1016/j.chroma.2014.04.015

Cited by 60 publications

(21 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is of importance for development of the suitable quality standard and control strategy, study of pharmacokinetics, and interpretation of therapeutic character of TCM [19, 20]. As a marketed product in China, the quality control system of TSC is still to be improved and its pharmacokinetics and action mechanism are not completely clear.…”

Section: Resultsmentioning

confidence: 99%

Chemical Profiling of an Antimigraine Herbal Preparation, Tianshu Capsule, Based on the Combination of HPLC, LC‐DAD‐MSⁿ, and LC‐DAD‐ESI‐IT‐TOF/MS Analyses

Liang

Gao

Chen

et al. 2014

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Chemical profiling is always the first task in the standardization and modernization of Traditional Chinese Medicine. HPLC and LC-MS were employed to find out the common chromatographic peaks in various batches of Tianshu Capsule (TSC) and the contribution of the characteristic peaks from individual herbs to the whole chromatographic profile of TSC sample. A total of 38 constituents were identified in TSC sample based on the comparison of retention time and UV spectra with authentic compounds as well as by summarized MS fragmentation rules and matching of empirical molecular formula with those of published components. This is the first systematic report on the chemical profiling of the commercial TSC product, which provides the sufficiently chemical evidence for the global quality evaluation of TSC products.

show abstract

Section: Resultsmentioning

confidence: 99%

Chemical Profiling of an Antimigraine Herbal Preparation, Tianshu Capsule, Based on the Combination of HPLC, LC‐DAD‐MSⁿ, and LC‐DAD‐ESI‐IT‐TOF/MS Analyses

Liang

Gao

Chen

et al. 2014

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…However, among the compounds studied, lupinifolin (5) which has the highest antibacterial and anticancer activities is most relevant to the bioactivities of this plant. As the batch-to-batch consistency of herbal products could be affected by many factors, for instance genetic and ecological factors, quality control has become an important requirement to support the clinical applications (Shi et al, 2014). Herbal products contain normally a complex mixture of phytochemicals, therefore selection of appropriate marker compounds that are relevant to the biological activities is currently an important approach to their quality control (Shi et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…As the batch-to-batch consistency of herbal products could be affected by many factors, for instance genetic and ecological factors, quality control has become an important requirement to support the clinical applications (Shi et al, 2014). Herbal products contain normally a complex mixture of phytochemicals, therefore selection of appropriate marker compounds that are relevant to the biological activities is currently an important approach to their quality control (Shi et al, 2014). Similarly, in order to facilitate an efficient standardization procedure for the future development of A. myriophylla extract as an herbal product, it is essential to define chemical markers responsible for its therapeutic activity.…”

Section: Discussionmentioning

confidence: 99%

Chemical constituents and biological activities ofAlbizia myriophyllawood

Joycharat

Boonma

Thammavong

et al. 2015

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Albizia myriophylla Benth (Leguminosae) is a medicinal plant widely used in Thailand and other Asian countries as a folk medicine remedy for many ailments. Objective: The objective of this study is to investigate the chemical compositions, antibacterial activity, and cytotoxicity of A. myriophylla wood. Materials and methods: The structure identification of the isolated compounds was established using spectroscopic methods. In vitro antibacterial activity against Streptococcus mutans, Staphylococcus aureus, and Bacillus cereus and the cytotoxicity against KB cells of extracts and compounds from A. myriophylla were performed using broth microdilution and resazurin microplate assays, respectively. The lupinifolin content in A. myriophylla extracts was quantified by high-performance liquid chromatography. Results: A rare flavan-3,4-diol (1) together with eight known compounds (2-9) were isolated from the wood of A. myriophylla. Compounds 4-9 exhibited anti-S. mutans activity, of which lupinifolin (5) was the most potent with an MIC value of 0.98 mg/mL, followed by its dihydroxy derivative 4 with an MIC value of 62.5 mg/mL. Compounds 4 and 5 also displayed marked antibacterial activity against B. cereus and S. aureus (MIC value 15.63-125 mg/mL) and showed strong cytotoxic activity against KB cells (IC 50 value 4.95-12.55 mg/mL). The lupinifolin contents in ethanol extracts from two different collections of this plant originating from central and southern Thailand were 93.85 and 0.04 mg/g, respectively. Conclusion: This is the first report of compounds 1-4 from A. myriophylla. Compounds 4 and 5 showed potent antibacterial and cytotoxic activities compared with other isolates. The anti-S. mutans activity of A. myriophylla extracts seems to be related to the lupinifolin content.

show abstract

“…Given the increasing global demand for herbal medicines, QC has become increasingly important in business and clinical applications . Herbal patent medicines contain relatively unrefined mixtures of phytochemical compounds, causing their QC to be highly challenging .…”

Section: Introductionmentioning

confidence: 99%

Qualitative and quantitative analysis of multiple components for quality control of Deng‐Zhan‐Sheng‐Mai capsules by ultra high performance liquid chromatography tandem mass spectrometry method coupled with chemometrics

Chen

2016

J of Separation Science

View full text Add to dashboard Cite

Deng-Zhan-Sheng-Mai capsules are a well-known traditional Chinese patent medicine that was developed in China for the treatment of ischemic stroke. Its quality control focuses on Erigerontis Herba but ignores the contributions of Ginseng Radix et Rhizoma, Schisandrae Chinensis Fructus, and Ophiopogonis Radix. To improve the quality standards for this medicine, this work reports the application of a systematic ultra high performance liquid chromatography tandem mass spectrometric method coupled with chemometrics. Three qualitative and quantitative parameters are established for the evaluation of quality: chemical profiling, the relationship between the contents of 18 compounds and the antioxidant activity, and chemometric analysis. A total of 55 compounds, including 20 phenolic acids, 10 flavonoids, 15 saponins, and 10 lignans, were identified. The method for the quantitative determination of the aforementioned 18 compounds was validated. The limit of quantification ranged from 0.13 to 9.60 ng/mL. The overall recoveries ranged from 95.31 to 103.54%. Hierarchical cluster analysis and principal component analysis were applied to the data of 18 components in ten batches of samples. Nine compounds, including scutellarin, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, ginsenoside Rb1, ginsenoside Re, ginsenoside Rg1, ophiopogonin D, schisandrin, and schisandrol B, are suggested as chemical markers for evaluating the quality.

show abstract

Identification of effective combinatorial markers for quality standardization of herbal medicines

Cited by 60 publications

References 24 publications

Chemical Profiling of an Antimigraine Herbal Preparation, Tianshu Capsule, Based on the Combination of HPLC, LC‐DAD‐MSⁿ, and LC‐DAD‐ESI‐IT‐TOF/MS Analyses

Chemical Profiling of an Antimigraine Herbal Preparation, Tianshu Capsule, Based on the Combination of HPLC, LC‐DAD‐MSⁿ, and LC‐DAD‐ESI‐IT‐TOF/MS Analyses

Chemical constituents and biological activities ofAlbizia myriophyllawood

Qualitative and quantitative analysis of multiple components for quality control of Deng‐Zhan‐Sheng‐Mai capsules by ultra high performance liquid chromatography tandem mass spectrometry method coupled with chemometrics

Contact Info

Product

Resources

About

Identification of effective combinatorial markers for quality standardization of herbal medicines

Cited by 60 publications

References 24 publications

Chemical Profiling of an Antimigraine Herbal Preparation, Tianshu Capsule, Based on the Combination of HPLC, LC‐DAD‐MSn, and LC‐DAD‐ESI‐IT‐TOF/MS Analyses

Chemical Profiling of an Antimigraine Herbal Preparation, Tianshu Capsule, Based on the Combination of HPLC, LC‐DAD‐MSn, and LC‐DAD‐ESI‐IT‐TOF/MS Analyses

Chemical constituents and biological activities ofAlbizia myriophyllawood

Qualitative and quantitative analysis of multiple components for quality control of Deng‐Zhan‐Sheng‐Mai capsules by ultra high performance liquid chromatography tandem mass spectrometry method coupled with chemometrics

Contact Info

Product

Resources

About

Chemical Profiling of an Antimigraine Herbal Preparation, Tianshu Capsule, Based on the Combination of HPLC, LC‐DAD‐MSⁿ, and LC‐DAD‐ESI‐IT‐TOF/MS Analyses

Chemical Profiling of an Antimigraine Herbal Preparation, Tianshu Capsule, Based on the Combination of HPLC, LC‐DAD‐MSⁿ, and LC‐DAD‐ESI‐IT‐TOF/MS Analyses