Identification of Echinococcus granulosus Genotypes G1 and G3 by SNPs Genotyping Assays

Bonelli, Piero; Giudici, Silvia Dei; Peruzzu, Angela; Mura, L.; Santucciu, Cinzia; Maestrale, Caterina; Masala, Giovanna

doi:10.3390/pathogens10020125

Cited by 12 publications

(8 citation statements)

References 32 publications

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“…Hence, G1 has been recognized as the predominant genotype in different intermediate hosts with a cosmopolitan distribution since it can be found in different countries are continents, such as Brazil [ 58 ], China [ 59 ], Turkey [ 60 ], Iran [ 61 ], and Europe [ 62 ]. Moreover, the presence of genotype G1 was detected with high prevalence in Mediterranean countries [ 41 ], and in particular, the island of Sardinia in Italy is considered an hyperendemic area, as described in a recent study performed of 28 hydatid cysts collected from intermediate hosts of different animal species [ 63 ]. Nevertheless, there are few other countries, such as Pakistan [ 64 ] and India [ 65 ], in which the prevalent E. granulosus s.s. genotype is represented by G3.…”

Section: Discussionmentioning

confidence: 99%

Genetic Characterization of Echinococcus granulosus sensu stricto Isolated from Human Cysts from Sardinia, Italy

Santucciu¹,

Bonelli²,

Peruzzu³

et al. 2023

Diseases

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This study involved 20 patients affected by cystic echinococcosis (CE) who were referred to different hospitals of Sardinia (Italy) from 2017 to 2022. By means of a multidisciplinary approach, diagnosis was confirmed for CE in 18 patients and for different aetiologies in two subjects. Moreover, serology was positive for 15 subjects. Since multiple CE cysts were found in five patients, a total of 27 lesions were collected; however, only one for each patient was investigated for genetic characterization of E. granulosus s.s. DNA isolates. Our results included 15 fertile cysts that underwent DNA extraction and amplification by three different PCRs targeting nuclear (calreticulin) and mitochondrial genes (cox1 and nad5). DNA was sequenced, and by neighbour-joining phylogenetic trees we determined 10 G1 and five G3 genotypes previously reported in Sardinia. These sequences were used to construct a network, along with those circulating in Mediterranean areas. The haplotype network calculated on cox1 evidenced seven different haplotypes of the 15 isolates, with SAR2 the most represented, carried by seven cysts, and SAR17 never described in the Mediterranean area. Meanwhile, the nad5 sequences showed the most common haplotype as nd5SAR7, as well as two new haplotypes not previously described, nd5SAR13, isolated from a Sardinian patient, and nd5SAR14, isolated from a Romanian patient.

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Section: Discussionmentioning

confidence: 99%

Genetic Characterization of Echinococcus granulosus sensu stricto Isolated from Human Cysts from Sardinia, Italy

Santucciu¹,

Bonelli²,

Peruzzu³

et al. 2023

Diseases

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“…Furthermore, the identification of the E. granulosus s.s. species and G1 genotype performed via RT-PCR give important epidemiological information. As broadly reported, G1 is the most represented genotype of intermediate hosts in the Sardinian hyperendemic area [ 22 ] and in other Mediterranean and European countries, comprising Romania [ 36 , 37 ], as well as the majority of countries worldwide [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently, an RT-PCR [ 22 ] was performed on three different single nucleotide polymorphisms (SNPs) ( Table 1 ) for the determination of E. granulosus sensu stricto ( s.s. ) and the distinction between G1 and G3 genotypes.…”

Section: Case Descriptionmentioning

confidence: 99%

Environmental Influence on the Occurrence of Multi-Organ Cystic Echinococcosis Infection in a Patient from Sardinia, Italy

Santucciu¹,

Ferrari

Grimaldi

et al. 2023

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An uncommon clinical case of an adult woman who was referred to the hospital with severe symptoms attributable to cystic echinococcosis (CE) is described in this report. According to a questionnaire, the subject was exposed to a high risk of infection since she was employed on a farm about 20 years before diagnosis. She lived close to several animal species and handled vegetables in inadequate hygienic conditions. Medical and laboratory investigations confirmed the presence of massive echinococcal cystic lesions in each lung and in the liver. Given the peculiarity of the case, pharmacological and surgical treatments were the only conceivable option. The association of pharmacological treatment, surgery, and interventional radiology procedure represented a reliable and effective way to handle a complex case of human hydatidosis. A multi-disciplinary approach was mandatory, resulting in a clear and conclusive diagnosis of CE caused by the zoonotic parasite E. granulosus sensu stricto of the G1 genotype.

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“…The E. granulosus sensu lato genotypes have a large variation in their host selectivity and pathogenicity 1 . Therefore, they are separated into 5 species 1,10,12 . These species are E. granulosus sensu stricto (G1/G3), E. intermedius (G6/G7), E. ortleppi (G5), E.canadensis (G8/G10) and E. equinus (G4).…”

Section: Introductionmentioning

confidence: 99%

Complete characterization of NADH dehydrogenase subunit 1 gene in human hydatid cysts

AL-Asadi,

Awad

2024

Baghdad Sci.J

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Echinococcus granulosus sensu stricto possesses very similar strains G1 and G3. Mitochondrial genes from NADH dehydrogenase (NAD) family have prominent roles in the identification of very similar strains G1 and G3. Thus, the present study mainly aimed to characterize the whole NAD1 gene (from the start codon to the stop codon) of hydatids isolated from humans. Hydatids were surgically isolated from the livers of patients, who are from Basrah and Maysan provinces, at Al-Sadir Teaching Hospital in Basrah province, Iraq. Protoscoleces were aseptically isolated from hydatid cysts and were used to extract DNA. Specific primers were employed to amplify the total NAD1 gene. A consensus sequence of nucleotides was separately generated from Basrah and Maysan sample sequences. Each consensus sequence had 1038 bp and it contained a start codon GTG at residues 87- 89 and a stop codon TAA at residues 978 – 980. The present study results displayed that each deduced amino acid sequence, predicated from a consensus sequence of nucleotides, had 297 amino acids. Furthermore, bioinformatics analysis revealed that each deduced amino acid sequence included a residue isoleucine (I188). This residue is only retained in the NAD1 protein of the G1 strain. The current study results also showed a predicated structural model of Basrah and Maysan NAD1 proteins and both of which had very similar homology models and the important residue I188 was located in the α10 helix. In total, for the first time in Iraq, this study characterized the whole NAD1 genes of E. granulosus protoscoleces and confirmed these NAD1 genes belonging to the G1 sheep strain.

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Identification of Echinococcus granulosus Genotypes G1 and G3 by SNPs Genotyping Assays

Cited by 12 publications

References 32 publications

Genetic Characterization of Echinococcus granulosus sensu stricto Isolated from Human Cysts from Sardinia, Italy

Genetic Characterization of Echinococcus granulosus sensu stricto Isolated from Human Cysts from Sardinia, Italy

Environmental Influence on the Occurrence of Multi-Organ Cystic Echinococcosis Infection in a Patient from Sardinia, Italy

Complete characterization of NADH dehydrogenase subunit 1 gene in human hydatid cysts

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