Identification of dysregulated <scp>miRNAs</scp>‐genes network in ovarian cancer: An integrative approach to uncover the molecular interactions and oncomechanisms

Kadkhoda, Sepideh; Darbeheshti, Farzaneh; Tavakkoly‐Bazzaz, Javad

doi:10.1002/cnr2.1286

Cited by 7 publications

(5 citation statements)

References 66 publications

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“…Thus, as the disease progresses with increasing aggressiveness and Gleason score, the expression of miRNA 4510 also increases in PCa serum. Its oncogenic activity has been reported in stage IA lung adenocarcinoma and ovarian cancer by Notch3 targeting [15,16]. There have been several studies to report its downregulation in various other cancers like colorectal, recurrent breast, bladder, and hepatocellular carcinoma [17][18][19].…”

Section: Discussionmentioning

confidence: 99%

Discovery and Validation of Novel microRNA Panel for Non-Invasive Prediction of Prostate Cancer

Kumari,

Manoj,

Rungta

et al. 2024

Cureus

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Background: Early diagnosis remains a challenge for prostate cancer (PCa) due to molecular heterogeneity. The purpose of our study was to explore the diagnostic potential of microRNA (miRNA) in both tissue and serum that may aid in the precise and early clinical diagnosis of PCa.Materials and methods: The miRNA expression pattern analysis was carried out in 250 subjects (discovery and validation cohort). The Discovery Cohort included the control (n = 30) and PCa (n = 35) subjects, while the Validation Cohort included the healthy control (n = 60), benign prostate hyperplasia (BPH) (n = 55), PCa (n = 50), and castration-resistant PCa (CRPC) (n = 20) patients. The expression analysis of tissue (Discovery Cohort) and serum (Validation Cohort) was carried out by quantitative polymerase chain reaction (qPCR). The diagnostic biomarker potential was evaluated using receiver operating characteristics (ROC). Bioinformatic tools were used to explore and analyze miRNA target genes.Results: MiRNA 4510 and miRNA 183 were significantly (p<0.001) upregulated and miRNA 329 was significantly (p<0.0001) downregulated in both PCa tissue and serum. ROC curve analysis showed excellent non-invasive biomarker potential of miRNA 4510 in both PCa (area under the curve (AUC) 0.984; p<0.001) and CRPC (AUC 0.944; p<0.001). The panel of serum miRNAs (miRNA 183 and miRNA 4510) designed for PCa had significant and greater AUC with both 100% sensitivity and specificity. Computational analysis shows that the maximum number of target genes are transcription factors that regulate oncogenes and tumor suppressors.Conclusion: Based on ROC curve analysis, miRNAs 4510, 329, and 711 were identified as potential noninvasive diagnostic biomarkers in the early detection of PCa. Our findings imply that a panel of miRNAs 183 and 4510 has high specificity for distinguishing PCa from healthy controls and providing therapeutic targets for better and earlier PCa therapy.

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Section: Discussionmentioning

confidence: 99%

Discovery and Validation of Novel microRNA Panel for Non-Invasive Prediction of Prostate Cancer

Kumari,

Manoj,

Rungta

et al. 2024

Cureus

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“…TFAP2C is thought to participate pathogenesis of cancers, including breast cancer [21], bladder cancer [22], ovarian cancer [23] and lung carcinoma [24]. Down-regulation of TFAP2C reduces the proliferation, migration, and invasion, and triggers apoptosis of breast cancer cells [25].…”

Section: Discussionmentioning

confidence: 99%

Transcription factor AP-2 gamma/Krüppel-like factor 10 axis is involved in miR-3656-related dysfunction of endothelial cells in hypertension

Ren

Shi

Liu

et al. 2023

Journal of Hypertension

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Background: Dysfunction of endothelial cells links to microvascular rarefaction, reflecting the pathogenesis of hypertension. Our previous studies found that miR-3656 reduces nitric oxide generation and von Willebrand factor (vWF) cleavage, thereby retarding blood flow and potentially increasing blood pressure. In this paper, we investigated mechanism of transcription regulation contributing to miR-3656-damaged endothelial cells in hypertension. Methods:The effects of miR-3656 on function of endothelial cells were analyzed on the basis of proliferation, migration, tube formation, and apoptosis. The mRNA level and protein level of genes were examined using quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. Dual-luciferase reporter assay was performed to confirm the binding between miR-3656 and 3' untranslated region (UTR) of transcription factor AP-2 gamma (TFAP2C). The binding between TFAP2C and the promoter region of Krppel-like factor 10 (KLF10) was confirmed by chromatin immunoprecipitation-qPCR assay.Results: miR-3656 impaired the cell proliferation, migration, tube formation, and apoptosis of endothelial cells. miR-3656 inhibited the expression of TFAP2C by directly targeting 3'UTR of TFAP2C; moreover, miR-3656induced injury of endothelial cells was rescued by TFAP2C overexpression. Furthermore, downregulated TFAP2C decreased KLF10 expression by binding to KLF10 promoter region, and upregulated KLF10 reversed the effects of silencing TFAP2C on endothelial cells. These inhibitory processes led to interference of miR-3656 to KLF10promoted function of endothelial cells. Conclusion:TFAP2C/KLF10 axis is involved in miR-3656related dysfunction of endothelial cells in hypertension. The 3'UTR of TFAP2C and KLF10 promoter region are the hubs of the TFAP2C/KLF10 axis.

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“…Bioinformatics studies and high-throughput sequencing are currently detecting a growing number of functional circRNAs [ 19 ]. Because of their unique characteristics and distinct advantages, such as their stability, long lifespan, time- and tissue-specific expression, they have turned into a novel hotspot in ncRNAs' research field to serve as potential biomarkers of cancer diagnosis and prognosis.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA hsa_circ_0044234 as distinct molecular signature of triple negative breast cancer: a potential regulator of GATA3

et al. 2021

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Background Circular RNAs (circRNAs) have been implicated in the initiation and development of breast cancer as functional non-coding RNAs (ncRNA). The roles of circRNAs as the competing endogenous RNAs (ceRNAs) to sponge microRNAs (miRNAs) have also been indicated. However, the functions of circRNAs in breast cancer have not been totally elucidated. This study aimed to explore the clinical implications and possible roles of circ_0044234 in carcinogenesis of the most problematic BC subtype, triple negative breast cancer (TNBC), which are in desperate need of biomarkers and targeted therapies. Methods The importance of circ_0044234 as one of the most dysregulated circRNAs in TNBC was discovered through microarray expression profile analysis. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to confirm the downregulation of circ_0044234 in triple negative tumors and cell lines versus non-triple negative ones. The bioinformatics prediction revealed that circ_0044234 could act as an upstream sponge in the miR-135b/GATA3 axis, two of the most dysregulated transcripts in TNBC. Results Our experimental investigation of circ_0044234 expressions in various BC subtypes as well as cell lines reveals that TNBC expresses circ_0044234 at a substantially lower level than non-TNBC. The ROC curve analysis indicates that it could be applied as a discriminative biomarker to identify TNBC from other BC subtypes. Moreover, circ_0044234 expression could be an independent prognostic biomarker in BC. Interestingly, a substantial inverse expression correlation was detected between circ_0044234 and miR-135b-5p as well as between miR-135b-5p and GATA3 in breast tumors. Conclusions The possible clinical usefulness of circ_0044234 as a promising distinct biomarker and upcoming therapeutic target for TNBC have been indicated in this research. Our comprehensive approach revealed the potential circ_0044234/miR135b-5p/GATA3 ceRNA axis in TNBC.

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Identification of dysregulated miRNAs‐genes network in ovarian cancer: An integrative approach to uncover the molecular interactions and oncomechanisms

Cited by 7 publications

References 66 publications

Discovery and Validation of Novel microRNA Panel for Non-Invasive Prediction of Prostate Cancer

Discovery and Validation of Novel microRNA Panel for Non-Invasive Prediction of Prostate Cancer

Transcription factor AP-2 gamma/Krüppel-like factor 10 axis is involved in miR-3656-related dysfunction of endothelial cells in hypertension

Circular RNA hsa_circ_0044234 as distinct molecular signature of triple negative breast cancer: a potential regulator of GATA3

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