Identification of duck IL-4 and its inhibitory effect on IL-17A expression in R. anatipestifer-stimulated splenic lymphocytes

Fernandez, Cherry P.; Afrin, Fahmida; Flores, Rochelle A.; Kim, Woo Hyun; Jeong, Junhui; Kim, Suk; Lillehoj, Hyun S.; Min, Wongi

doi:10.1016/j.molimm.2018.01.009

Cited by 13 publications

(9 citation statements)

References 57 publications

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“…In conclusion, DIM treatment appears to suppress the development of riemerellosis by reducing the bacterial burden and the expression of inflammatory cytokines in tissues of R. anatipestifer-infected ducks, resulting in higher survival rates. Moreover, given the marked upregulation of inflammatory cytokine expression in both R. anatipestifer-stimulated splenic lymphocytes and R. anatipestifer-infected ducks in our previous studies [15,16,42,48], the results of the present study further suggest that inhibition of inflammatory cytokine expression could significantly reduce economic losses associated with R. anatipestifer infection in farmed ducks.…”

Section: Plos Onesupporting

confidence: 71%

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Section: Plos Onesupporting

confidence: 71%

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“…Few studies demonstrated that suppression of proinflammatory cytokine expression alleviates R. anatipestifer infection [42,44,48]. Chickens infected with R. anatipestifer exhibited , and IL-1β (D) mRNA were normalized to that of β-actin and calibrated using the expression levels of unstimulated cultured splenic lymphocytes (NC).…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory activity of diindolylmethane alleviates Riemerella anatipestifer infection in ducks

Fernandez-Colorado¹,

Cammayo²,

Flores³

et al. 2020

PLoS ONE

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3,3’-Diindolylmethane (DIM) is found in cruciferous vegetables and is used to treat various inflammatory diseases because of its potential anti-inflammatory effects. To investigate effects of DIM in Riemerella anatipestifer-infected ducks which induce upregulation of inflammatory cytokines, ducks were treated orally with DIM at dose of 200 mg/kg/day and infected the following day with R. anatipestifer. Infected and DIM-treated ducks exhibited 14% increased survival rate and significantly decreased bacterial burden compared to infected untreated ducks. Next, the effect on the expression level of inflammatory cytokines (interleukin [IL]-17A, IL-17F, IL-6, IL-1β) of both in vitro and in vivo DIM-treated groups was monitored by quantitative reverse-transcription PCR (qRT-PCR). Generally, the expression levels of the cytokines were significantly reduced in DIM-treated splenic lymphocytes stimulated with killed R. anatipestifer compared to stimulated untreated splenic lymphocytes. Similarly, the expression levels of the cytokines were significantly reduced in the spleens and livers of DIM-treated R. anatipestifer–infected ducks compared to infected untreated ducks. This study demonstrated the ameliorative effects of DIM in ducks infected with R. anatipestifer. Thus, DIM can potentially be used to prevent and/or treat R. anatipestifer infection via inhibition of inflammatory cytokine expression.

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“…Duck splenic lymphocytes were obtained from a two-week-old healthy duck as previously described 52 . Briefly, aseptically collected spleens of ducks were gently separated using a cell strainer (SPL Life Science, Korea) to obtain a single cell suspension, and the splenic lymphocytes were collected using Ficoll-Paque PLUS (GE Health Life Sciences, UK) following the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

“…Splenic lymphocytes were resuspended to a concentration of 5 × 10 6 cells/ml and stimulated with 10 µg/ml concanavalin A (ConA; Amersham Bioscience, Sweden), 25 µg/ml polyinosinic:polycytidylic acid (poly I:C; Sigma-Aldrich, Germany), 10 µg/ml lipopolysaccharide (LPS from Escherichia coli , 0111:b4; Sigma-Aldrich), or heat-killed R . anatipestifer (1 × 10 6 CFU/ml) for 4, 8, and 24 h. Duck primary macrophages were obtained from spleens of 2-week-old ducks as previously described 52,53 . Briefly, spleens were homogenized and sieved using a syringe plunger and passed through a cell strainer to obtain a single cell suspension.…”

Section: Methodsmentioning

confidence: 99%

Riemerella anatipestifer infection in ducks induces IL-17A production, but not IL-23p19

Flores

Fernandez-Colorado

Afrin

et al. 2019

Sci Rep

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R. anatipestifer (RA) is one of the most harmful bacterial pathogens affecting the duck industry, and infection is associated with the production of proinflammatory cytokines, including IL-17A. Another proinflammatory cytokine, IL-23, is critical for the development of Th17 cells, which produce IL-17. However, IL-23 roles have not been studied in this infection. Here, we describe the identification and mRNA expression analysis of duck IL-23p19 (duIL-23p19) in splenic lymphocytes and macrophages stimulated with killed RA and in spleens of RA-infected ducks. Expression of duIL-23p19 transcript identified in this study was relatively high in livers of healthy ducks and was upregulated in mitogen-activated splenic lymphocytes as well as in splenic lymphocytes and macrophages stimulated with killed RA. In spleens of RA-infected ducks, expression levels of duIL-23p19 transcript were unchanged at all time points except on days 4 and 7 post-infection; however, duIL-17A and IL-17F expression levels were upregulated in both spleens of RA-infected ducks and splenic lymphocytes and macrophages stimulated with killed RA. In sera collected at 24 h after this infection, duIL-23p19 expression levels were unchanged, whereas IL-17A significantly upregulated. These results suggest that IL-23p19 does not play a critical role in the IL-17A response in early stages of RA-infected ducks.

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Identification of duck IL-4 and its inhibitory effect on IL-17A expression in R. anatipestifer-stimulated splenic lymphocytes

Cited by 13 publications

References 57 publications

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Anti-inflammatory activity of diindolylmethane alleviates Riemerella anatipestifer infection in ducks

Riemerella anatipestifer infection in ducks induces IL-17A production, but not IL-23p19

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