“…NGS technologies have become essential for genome analysis and SNP research, as well as gene discovery, comparative genomics, and studies on the regulation of gene expression (Abdelrahman, El‐Sayed, Sato, et al, ; Celesti, Celesti, Fazio, Bramanti, & Villari, ; Considine et al, ). The NGS‐based, that is, RNA sequencing‐based, transcriptome analysis is considered to be a superior approach for understanding gene function and the molecular basis of many cellular responses in plants exposed to abiotic and biotic stressors, compared with microarrays‐based one (Abdelrahman, Suzumura, et al, ; Abdelrahman, El‐Sayed, Sato, et al, ; Ha et al, ; Li, Cao, et al, ; Li, Wan, et al, ; O'Rourke et al, ). For example, some plant species have similar genomes but have very different gene expression profiles, reflecting a wide range of biochemical, physical, and developmental differences.…”