Identification of downy mildew resistance gene candidates by positional cloning in maize (<i>Zea mays</i> subsp. <i>mays</i>; Poaceae)

Kim, Jae Yoon; Moon, Jun-Cheol; Kim, Hyo Chul; Shin, Seung-Ho; Song, Kitae; Kim, Kyung‐Hee; Lee, Byung-Moo

doi:10.3732/apps.1600132

Cited by 7 publications

(6 citation statements)

References 26 publications

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“…Rpv11 , founded on chromosome 5 in a population from Regent, was related to low level of downy mildew resistance in some cultivars of Regent, Chardonnay and Solaris (Schwander et al, 2012 ; Van Heerden et al, 2014 ). Introduced from V. amurensis , dominant Rpv12 was detected in a chromosome 14 of Kozma 20-3 cultivar together with Rpv3-1 (Venuti et al, 2013 ; Kim et al, 2017 ; Possamai et al, 2020 ). Recently, linkage analysis identified three QTLs for P. viticola resistance: Rpv22 on LG 02, Rpv23 on LG15, and Rpv24 on LG18 (Fu et al, 2020 ).…”

Section: Disease Managementmentioning

confidence: 99%

“…Single-site fungicides provide both pre- and post-infection protection and include four main chemical classes: phenylamide compounds such as mefenoxam, metalaxyl, and benalaxyl (Magarey et al, 1991 ; Nicholas et al, 1994 ; Falk et al, 1996 ; Underdown et al, 2008 ); strobilurines such as azoxystrobin, trifloxystrobin, famoxadone, and fenamidone (Reuveni, 2001 ; Wong and Wilcox, 2001 ; Genet et al, 2006 ); the carboxylic acid amides like dimethomorph, iprovalicarb, flumorph, benthiavalicarb, mandipropamid (Wicks and Hall, 1990 ; Albert et al, 1991 ; Gisi et al, 2007 ; Reuveni, 2014 ; Nanni et al, 2016 ; Kim et al, 2017 ); and cyano-acetamide-based oxime carbonates like cymoxanil (Gullino et al, 1997 ; Genet and Vincent, 1999 ; Joshi, 2003 ). Both cymoxanil and chlorothalonil are specific, non-systemic, mildew fungicides and are curative if they are applied during the first few days post infection.…”

Section: Disease Managementmentioning

confidence: 99%

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Plasmopara viticola the Causal Agent of Downy Mildew of Grapevine: From Its Taxonomy to Disease Management

et al. 2022

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Plasmopara viticola (P. viticola, Berk. & M. A. Curtis; Berl. & De Toni) causing grapevine downy mildew is one of the most damaging pathogens to viticulture worldwide. Since its recognition in the middle of nineteenth century, this disease has spread from America to Europe and then to all grapevine-growing countries, leading to significant economic losses due to the lack of efficient disease control. In 1885 copper was found to suppress many pathogens, and is still the most effective way to control downy mildews. During the twentieth century, contact and penetrating single-site fungicides have been developed for use against plant pathogens including downy mildews, but wide application has led to the appearance of pathogenic strains resistant to these treatments. Additionally, due to the negative environmental impact of chemical pesticides, the European Union restricted their use, triggering a rush to develop alternative tools such as resistant cultivars breeding, creation of new active ingredients, search for natural products and biocontrol agents that can be applied alone or in combination to kill the pathogen or mitigate its effect. This review summarizes data about the history, distribution, epidemiology, taxonomy, morphology, reproduction and infection mechanisms, symptoms, host-pathogen interactions, host resistance and control of the P. viticola, with a focus on sustainable methods, especially the use of biocontrol agents.

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Section: Disease Managementmentioning

confidence: 99%

Section: Disease Managementmentioning

confidence: 99%

Plasmopara viticola the Causal Agent of Downy Mildew of Grapevine: From Its Taxonomy to Disease Management

et al. 2022

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“…DM disease was scored in the field using a modified spreader-row technique [2]. Susceptible genotypes (B73 and CML270) were used as two 5-m-long spreader rows with 20 plants per row after every 10th row of the test entries on the border rows of the experimental block ( Figure S1) [44,47]. Seeds of the spreader genotypes germinated after 7 days, then a DM-infected maize plant in a pot was placed in each experimental block for 2 weeks to infect the spreader plants.…”

Section: Evaluation Of the Rils For Dmmentioning

confidence: 99%

Identification and Validation of Candidate Genes Conferring Resistance to Downy Mildew in Maize (Zea mays L.)

Kim

Song

et al. 2020

Genes

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Downy mildew (DM) is a major disease of maize that causes significant yield loss in subtropical and tropical regions around the world. A variety of DM strains have been reported, and the resistance to them is polygenically controlled. In this study, we analyzed the quantitative trait loci (QTLs) involved in resistance to Peronosclerospora sorghi (sorghum DM), P. maydis (Java DM), and Sclerophthora macrospora (crazy top DM) using a recombinant inbred line (RIL) from a cross between B73 (susceptible) and Ki11 (resistant), and the candidate genes for P. sorghi, P. maydis, and S. macrospora resistance were discovered. The linkage map was constructed with 234 simple sequence repeat (SSR) and restriction fragment length polymorphism (RFLP) markers, which was identified seven QTLs (chromosomes 2, 3, 6, and 9) for three DM strains. The major QTL, located on chromosome 2, consists of 12.95% of phenotypic variation explained (PVE) and a logarithm of odds (LOD) score of 14.12. Sixty-two candidate genes for P. sorghi, P. maydis, and S. macrospora resistance were obtained between the flanked markers in the QTL regions. The relative expression level of candidate genes was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) using resistant (CML228, Ki3, and Ki11) and susceptible (B73 and CML270) genotypes. For the 62 candidate genes, 15 genes were upregulated in resistant genotypes. Among these, three (GRMZM2G028643, GRMZM2G128315, and GRMZM2G330907) and AC210003.2_FG004 were annotated as leucine-rich repeat (LRR) and peroxidase (POX) genes, respectively. These candidate genes in the QTL regions provide valuable information for further studies related to P. sorghi, P. maydis, and S. macrospora resistance.Genes 2020, 11, 191 2 of 20 is spread by oospores that survive in the soil [7] and can be spread through infected seeds or from plant-to-plant by airborne conidia. Because of the systemic nature of DM, susceptible lines usually die when infected in the seedling emergence stage, and when plants are infected during later growth stages, they cannot develop the maize ear despite having survived. At least six pathogens that cause DM infection of maize in Asia have been reported, including sorghum DM (P. sorghi (Weston & Uppal)), Philippine DM (P. philippinensis (Weston) Shaw), Java DM (P. maydis (Raciborski)), sugarcane DM (P. sacchari (Miyabe) Shirai and Hara), brown stripe DM (Sclerophthora rayssiae var. zeae), crazy top DM (S. macrospora), and Rajasthan DM (P. heteropogoni) [2,6,[8][9][10][11][12][13][14][15]. DM is widespread in tropical regions, although its origin is conjectural, and because of the diversity of the DM pathogens and their systemic nature, the development of resistant varieties is needed. Moreover, a renewed emphasis on cost-effectiveness and environmental safety that has brought about the application of DM management by the development of resistant varieties. According to studies on the interaction of maize and the pathogens, resistance to DM is polygenically controlled [7,13,[16][17][18]...

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“…The PVE diversity in the linkage area was caused by infection pathogenic factor to the response of the resistance maize lines. Based on previous study, Kim et al (2017) and Ruswandi et al (2015) have identified QTL for downy resistance on chromosome 1 using F2 and BC1F2 populations, respectively. Furthermore, it was also reported by Jampatong et al (2013) that QTL related to Pp in the RILs, Lohithaswa et al (2015) found QTL related to Ps (Peronosclerospora sorghi) in F2 population, and QTL linked Ph (Peronosclerospora heteropogoni) character was obtained BC1F2 population (Kim et al 2020).…”

Section: Discussionmentioning

confidence: 99%

QTL Mapping linked to downy mildew resistance genes in maize (Zea mays)

et al. 2020

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Abstract. Ashan MD, Miftahudin, Reflinur, Pabendon MB, Santoso SB, Salim A. 2020. QTL Mapping linked to downy mildew resistance genes in maize (Zea mays). Biodiversitas 21: 3735-3743. Downy Mildew (DM) caused by Peronosclerospora spp. is one of the most destructive diseases and problems in yield losses of maize production worldwide. Identification of molecular marker-linked to the DM resistance genes is considered as an important step in the improvement of the DM resistance nature in maize breeding program. The objective of the study was to identify molecular markers linked to the DM resistance genes in maize. A total of 198 F3 maize lines generated from a cross between R10-4430 and Kandora was used in microsatellite genetic map construction and the corresponding F3: 4 families were used in phenotypic evaluation to identify quantitative trait loci (QTLs) responsible for DM resistance-related trait. The entire genetic linkage map constructed with 28 SSR markers resulted in 460.3 cM with an average distance between markers of 26.85 cM. Seven main-effect QTLs controlling the DM resistance genes were identified in the entire genome map of F3 population with the phenotypic variation explained (PVE) values ranged from 43.35 to 53.71%. Among detected QTLs, three QTLs, qDM-Pp1a, qDM-Pp1b1, and qDM-Pp1b2 were detected on chromosome 1, one QTL, qDM-Pp5 was on chromosome 5, two QTLs, qDM-Pp6b1 and qDM-Pp6b2 were on chromosome 6 and one QTL, qDM-Pp10 was on chromosome 10. The effect of detected QTLs responsible for DM resistance trait ranged from 43.35 to 53.71% and those will be potential to be further used as molecular aided selection in maize breeding for DM resistance.

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Identification of downy mildew resistance gene candidates by positional cloning in maize (Zea mays subsp. mays; Poaceae)

Cited by 7 publications

References 26 publications

Plasmopara viticola the Causal Agent of Downy Mildew of Grapevine: From Its Taxonomy to Disease Management

Plasmopara viticola the Causal Agent of Downy Mildew of Grapevine: From Its Taxonomy to Disease Management

Identification and Validation of Candidate Genes Conferring Resistance to Downy Mildew in Maize (Zea mays L.)

QTL Mapping linked to downy mildew resistance genes in maize (Zea mays)

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