Identification of distinct N-glycosylation patterns on extracellular vesicles from small-cell and non–small-cell lung cancer cells

Kondo, Kosuke; Harada, Yoichiro; Nakano, Miyako; Suzuki, Takehiro; Fukushige, Tomoko; Hanzawa, Ken; Yagi, Hirokazu; Takagi, Koichi; Mizuno, Keiko; Miyamoto, Yasuhide; Taniguchi, Naoyuki; Kato, Koichi; Kanekura, Takuro; Dohmae, Naoshi; Machida, Kentaro; Maruyama, Ikuro; Inoue, Hiromasa

doi:10.1016/j.jbc.2022.101950

Cited by 22 publications

(16 citation statements)

References 67 publications

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“…Identification of N- linked glycosylation of proteins also provide new approach as biomarker. The small extracellular vesicles with N-glycosylation in secretome of lung cancer reflects lung cancer histology [35] .…”

Section: Post Translational Modificationmentioning

confidence: 99%

Unraveling the Post-Translational Modifications and therapeutical approach in NSCLC pathogenesis

Gulhane¹,

Singh²

2023

Translational Oncology

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Section: Post Translational Modificationmentioning

confidence: 99%

Unraveling the Post-Translational Modifications and therapeutical approach in NSCLC pathogenesis

Gulhane¹,

Singh²

2023

Translational Oncology

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“…Several experiments have shown that abnormal protein glycosylation is present in almost all CRDs, including COPD ( 20 ), asthma ( 21 , 22 ), CF ( 23 , 24 ), PAH ( 25 ), IPF ( 26 ), and lung cancer ( 27 – 29 ). For example, in COPD, an N-glycomics study found an abnormal N-glycosylation pattern in plasma proteins, with a decrease in low branching forms and increase in more complex forms ( 20 ).…”

Section: Aberrant Glycosylation In Crdsmentioning

confidence: 99%

Targeting protein glycosylation to regulate inflammation in the respiratory tract: novel diagnostic and therapeutic candidates for chronic respiratory diseases

2023

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Protein glycosylation is a widespread posttranslational modification that can impact the function of proteins. Dysregulated protein glycosylation has been linked to several diseases, including chronic respiratory diseases (CRDs). CRDs pose a significant public health threat globally, affecting the airways and other lung structures. Emerging researches suggest that glycosylation plays a significant role in regulating inflammation associated with CRDs. This review offers an overview of the abnormal glycoenzyme activity and corresponding glycosylation changes involved in various CRDs, including chronic obstructive pulmonary disease, asthma, cystic fibrosis, idiopathic pulmonary fibrosis, pulmonary arterial hypertension, non-cystic fibrosis bronchiectasis, and lung cancer. Additionally, this review summarizes recent advances in glycomics and glycoproteomics-based protein glycosylation analysis of CRDs. The potential of glycoenzymes and glycoproteins for clinical use in the diagnosis and treatment of CRDs is also discussed.

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“…Small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) represent the two most common subtypes of lung cancer. , Notably, there are distinct differences in the N -glycomes of EVs released from SCLC cells and NSCLC cells. The structural motifs of N -glycans found in SCLC-released EVs resembled the brain N -glycans, while NSCLC-released EVs predominantly featured lung-specific N -glycans characterized by core fucosylation, a feature associated with NSCLC . Furthermore, lectin array analysis revealed the selective expression of integrins between these two cell types.…”

Section: Ev Glycosylation In Cancersmentioning

confidence: 99%

Comprehensive Overview the Role of Glycosylation of Extracellular Vesicles in Cancers

Wu,

Gao

2023

ACS Omega

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Extracellular vesicles (EVs) are membranous structures secreted by various cells carrying diverse biomolecules. Recent advancements in EV glycosylation research have underscored their crucial role in cancer. This review provides a global overview of EV glycosylation research, covering aspects such as specialized techniques for isolating and characterizing EV glycosylation, advances on how glycosylation affects the biogenesis and uptake of EVs, and the involvement of EV glycosylation in intracellular protein expression, cellular metastasis, intercellular interactions, and potential applications in immunotherapy. Furthermore, through an extensive literature review, we explore recent advances in EV glycosylation research in the context of cancer, with a focus on lung, colorectal, liver, pancreatic, breast, ovarian, prostate, and melanoma cancers. The primary objective of this review is to provide a comprehensive update for researchers, whether they are seasoned experts in the field of EVs or newcomers, aiding them in exploring new avenues and gaining a deeper understanding of EV glycosylation mechanisms. This heightened comprehension not only enhances researchers’ knowledge of the pathogenic mechanisms of EV glycosylation but also paves the way for innovative cancer diagnostic and therapeutic strategies.

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Identification of distinct N-glycosylation patterns on extracellular vesicles from small-cell and non–small-cell lung cancer cells

Cited by 22 publications

References 67 publications

Unraveling the Post-Translational Modifications and therapeutical approach in NSCLC pathogenesis

Unraveling the Post-Translational Modifications and therapeutical approach in NSCLC pathogenesis

Targeting protein glycosylation to regulate inflammation in the respiratory tract: novel diagnostic and therapeutic candidates for chronic respiratory diseases

Comprehensive Overview the Role of Glycosylation of Extracellular Vesicles in Cancers

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