Identification of Differentially Expressed Putative Nitrate Transporter Genes in Lettuce

Razgallah, Nesrine; Chikh-Rouhou, H.; Abid, Ghassen; Mahmoud, Mahmoud A.

doi:10.1080/19315260.2017.1309614

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…The positive lower nitrate accumulation recorded in “Aroma 2” emphasizes the genotype’s key role in accumulating this potentially risky dietary compound for human health ( Colla et al, 2018 ). This may be connected to a different expression of genes involved in nitrate transport, as shown in lettuce ( Razgallah et al, 2017 ) and/or a higher nitrate reductase activity ( Luo et al, 2006 ). Magnesium is a central cation of the chlorophyll molecule and involved in RuBisCO activation, promoting CO 2 assimilation ( Karthika et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Morpho-Physiological Responses and Secondary Metabolites Modulation by Preharvest Factors of Three Hydroponically Grown Genovese Basil Cultivars

et al. 2021

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Sweet basil (Ocimum basilicum L.) is an economically important leafy vegetable especially in Mediterranean countries. In Italian gastronomy, the large elliptical leaves of the Genovese type are mostly used for the well-known pesto sauce, and almost all (>90%) professional production is for the food industry. The growing demand for fresh leaves with standardized technological and sensory characteristics has prompted basil producers to adopt advanced cultivation methods such as the floating raft system (FRS). The aim of this study was to evaluate the productive, qualitative, and physiological performance of three Genovese basil cultivars (“Aroma 2,” “Eleonora,” and “Italiano Classico”) in two successive harvests and at two densities (159 and 317 plants m–2). Caffeic, chicoric, rosmarinic, and ferulic acid were determined through the high-performance liquid chromatography (HPLC) system, whereas the extraction and quantification of the volatile organic compounds (VOCs) were performed by solid-phase microextraction (SPME) and gas chromatography coupled to a mass spectrometer (GC/MS). “Aroma 2” showed the highest fresh yield and photosynthetic rate together with the lowest nitrate content. For all the tested cultivars, the higher density, while reducing the number of leaves per plant, resulted in higher fresh and dry production per unit area, without altering the aroma profile. Successive harvests resulted in a significant increase in both the yield (37.5%) and the total phenolic acids (75.1%) and favored Eucalyptol and 1-octen-3-ol accumulation (+25.9 and +15.1%, respectively). The here presented comprehensive and multifactorial assessment of the productive and qualitative response of basil provides evidence of the positive effects (from biomass to specialized metabolites) that can be obtained from the management of the pre-harvest factors in soilless cultivation. In addition, it also highlights the role and constraints of the genetic factor in the observed response. We also discuss the implications of our work considering the impact for the food processing industry. Future research may explore the phenolic acids accumulation as a possible fortification means to extend the pesto sauce shelf life, reducing the need of added antioxidants and thermal processing.

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Section: Discussionmentioning

confidence: 99%

Morpho-Physiological Responses and Secondary Metabolites Modulation by Preharvest Factors of Three Hydroponically Grown Genovese Basil Cultivars

et al. 2021

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“…Luo et al [46] compared two pakchoi cultivars (Brassica rapa L.) showing that the low nitrate-accumulating cultivar Shanghaiquing had a significantly higher nitrate reductase activity and gene expression of the putative nitrate reductase gene compared to high nitrateaccumulating cultivar Liangbaiye-1. Razgallah et al [47] compared four lettuce cultivars and found that high nitrate accumulators had higher nitrate transporter transcripts than the low accumulators, suggesting a stronger ability to take up and transport nitrates in the higher accumulators. Moreover, the nitrate concentration in aerial plant organs was higher than in the roots of rape, cabbage, and spinach [48].…”

Section: Nitrogen Protein Anions and Organic Acids Contentmentioning

confidence: 99%

Yield, Fructans Accumulation, and Nutritional Quality of Young Chicory Plants as Related to Genotype and Nitrogen Fertilization

et al. 2023

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Growth and quality attributes were quantified in Cichorium intybus L. and Cichorium endivia L. in response to the nitrate supply. Chicory was grown in Italy, in a cold greenhouse from the 11 of February 2020, in a pot with commercial soil and sand with and without 12 mM of Ca(NO3)2. Seventy-six days after sowing, the growth variables, contents of leaf and root carbohydrates (glucose, fructose sucrose starch and fructans), carbon, nitrate sulphate and phosphate were measured. Fertilization significantly increased the yield and specific leaf dry weight (SLDW) of the C. endivia. The shoot/root ratio was increased in C. intybus with high N; this also increased the carbohydrate content in leaves and roots of C. endivia compared to the value measured in C. intybus. The interaction between fertilization and genotype significantly affected fructans and nitrate accumulation in taproots. Fertilization decreased the sulphate and phosphate contents in the leaves and roots of both chicory species. The yield and quality of young chicory plants can be modulated by species selection and nitrate fertilization. The genotype and nitrogen supply interact in modulating the yield and the inulin and nitrate accumulation in the taproot, thereby affecting its nutritional value and representing a powerful tool to cultivate this new produce for healthy human nutrition.

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“…В ряде работ Reinink K. и др., показали, что наследование содержания нитратов в салате следует аддитивно-доминантной модели [18,19,20,]. При изучении сортов салата: Виталия (сортотип Баттерхед), Великие озера (сортотип Айсберг), салата -ромэн, выяснилось, что гены LsNRT1 и LsNRT2 кодируют транспорт нитратов с низким и высоким содержанием нитратов [21].…”

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Influence of nitrogen stress on nitrate accumulation and yield of lettuce varieties

Volkova

2023

Ovoŝi Rossii

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Relevance. Many authors note the existence of significant genotypic differences in the assimilation of nitrogen and the accumulation of nitrates by lettuce, point to the additive-dominant nature of the inheritance of the trait. The aim of the work was to evaluate the response of lettuce of various cultivars to mineral nutrition stress created against a nitrogen-deficient background (N0) and with an increased dose of nitrogen (N200).Material and Methods. In a microfield experiment on well-cultivated soddy-podzolic soil, a collection of lettuce varieties belonging to the main cultivated variety types was studied. The varieties differed in geographical origin, early maturity and morphological characteristics.Results. Differences in yield between varieties against the background of N200 were 4.5 times, and against the background of N0 – 5.7 times. Against an increased background of nitrogen nutrition, the highest yield – 2.22-2.64 kg/m2 was formed by the following varieties: All the Year, Quedlinburger Dickkopf, Berlin Yellow, Great lakes. Against a nitrogen-deficient background, Azart and Odessa Kucheryavets varieties stood out in terms of yield, which absorbed soil nitrogen better than others. The content of nitrates in lettuce plants of different varieties varied more strongly when grown on the background of N200 and ranged from 10.7 to 13.6 times (on average over 2 years – 11,2 times), on the control of N0 – 33 times. Varieties accumulating maximum NO3 under conditions of excess nitrogen nutrition were revealed. Lettuce Romaine (Lactuca romana) – 353 mg/kg and White paris cus (198 mg/kg) had a low content of NO3. The period of the maximum increase in biomass also corresponded to the maximum decrease in NO3 in plants. Power function equations were calculated to estimate the rate of change in biomass and NO3 content. The results indicate that there may be some scope for NO3 reduction in lettuce through cultivar selection or breeding.

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Identification of Differentially Expressed Putative Nitrate Transporter Genes in Lettuce

Cited by 6 publications

References 25 publications

Morpho-Physiological Responses and Secondary Metabolites Modulation by Preharvest Factors of Three Hydroponically Grown Genovese Basil Cultivars

Morpho-Physiological Responses and Secondary Metabolites Modulation by Preharvest Factors of Three Hydroponically Grown Genovese Basil Cultivars

Yield, Fructans Accumulation, and Nutritional Quality of Young Chicory Plants as Related to Genotype and Nitrogen Fertilization

Influence of nitrogen stress on nitrate accumulation and yield of lettuce varieties

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